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저빈도 전기자극이 뒷다리 무부하 상태의 흰쥐 골격근 IGF-1 발현에 미치는 영향

Influence of Low-Frqeuency Electrical Stimulation on IGF-1 Expression of Rat Skeletal Muscle During Hindlimb Unloading

강종호
특수교육재활과학연구소 (The Research Institute for Special Education & Rehabilitation Science)|2009.12|특수교육재활과학연구|제48권 제4호|pp. 1-18

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참고문헌

키워드

IGF-1electrical stimulation근위축muscle atrophy전기자극
주제분류기타사회과학

초록

본 연구의 목적은 실험동물의 뒷다리를 2주간 현수시키는 동안에 적용한 저빈도 전기자극이 근육의 성장을 자극시키는 동화성 조절 인자로 알려진 Insuline-like growth factor-1(IGF-1) 발현에 미치는 영향을 알아보는데 있다. 이를 위해서 300-350g의 Sprague-Dawley계 수컷 흰쥐 25마리를 14일간 뒷다리 현수 처치한 HLS군, 14일간 자유롭게 사육한 WB군, 14일 현수 기간 중 14일간 전기자극을 실시한 ES14군, 14일 현수 기간 중 11일 전기자극한 ES11군, 14일 현수 기간 중 7일간 전기자극한 ES7군에 5마리씩 무작위로 배치하였다. 저빈도 전기자극은 비복근에 적용하였으며, 16Hz의 비대칭성 이상성 맥동파를 1일 15분씩 적용하였다. 실험 결과, WB군과 HLS군에서 유의하게 낮은 IGF-1발현이 관찰되었으며, ES14군, ES11군, ES7군에서는 유의하게 높은 IGF-1 발현이 관찰되었다. 이 결과는 14일, 11일, 7일의 16Hz 저빈도 전기자극의 단백질 파괴 지연 효과를 보여주는 것이다.

Abstract

The purpose of this study was to examin the effect of low frequency electrical stimulation(LFES) during hindlimb suspension on insuline-like growth factor-1 expression as an anabolic regulator of skeletal muscle growth of rat gastrocnemius. Sprague-Dawley rats(body weight 300- 350g) were randomly assigned to five groups: a HLS(n=5) that were hindlimb suspended for 14 days, a WB(n=5) that kept as control, a ES14 that were hind limb suspended for 14 days with pre-application of LFES for 14 days, a ES11 that were hindlimb suspended for 14 days with pre-application of LFES for 11 days, a ES7 that were hindlimb suspended for 14 days with pre-application of LFES for 7 days. Electrical stimulation was applied to the gastrocnemius belly for 15 minutes per days. Electrical stimulation parameters were set to have asymmetric biphasic pulse wave at 16Hz frequency. IGF-1 were significantly decreased expression in WB, HLS groups, whereas IGF-1 were increased in ES14, ES11, ES7 groups. This indicated that LFES for 14, 11, 7 days could prevent muscle degradation.

참고문헌 (46)

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