치면착색제를 이용한 광역동 치료의 항균 효과
Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent
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초록
이 연구의 목적은 치면착색제인 10 - 20 mM erythrosine을 광감각제로 사용한 광역동 치료의 항균 효과를 평가하는 것이다. Streptococcus mutans, Lactobacillus casei, Candida albicans 를 포함하는 다종 우식원성 세균막을 hydroxyapatite disc에 형성하였다. 광감각제로 20 μM, 10 mM, 20 mM erythrosine을 3분간 적용 후 24초 동안 광조사를 시행하였다. 각 실험군의 Colony-forming unit (CFU)을 측정하고 세균막을 Confocal laser scanning microscopy (CLSM)을 이용하여 관찰하였다. 10 - 20 mM erythrosine을 광감각제로 사용한 광역동 치료 실험군에서 CFU의 현저한 감소가 관찰되었고, 그 결과는 CLSM 관찰에서도 확인되었다. 이번 연구를 통해 치과 임상에서 사용되는 치면착색제를 광감각제로 이용한 광역동 치료의 높은 항균효과를 확인할 수 있었다.
Abstract
The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer. Multispecies cariogenic biofilms containing Streptococcus mutans , Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM. This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.
참고문헌 (13)
- Sharman WM, "Photodynamic therapeutics : basic principles and clinical applications", Drug Discov Today, 4, pp.507~517 (1999)
- Konopka K, "Photodynamic therapy in dentistry", J Dent Res, 86, pp.694~707 (2007)
- Wood S, "Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms", J Antimicrob Chemother, 57, pp.680~684 (2006)
- 정지숙, "THE EFFECT OF PHOTODYNAMIC THERAPY ON THE VIABILITY OF STREPTOCOCCUS MUTANS ISOLATED FROM ORAL CAVITY", 대한소아치과학회지, 39(3), pp.233~241 (2012), 대한소아치과학회[DOI]
- 최서정, "Streptococcus mutans biofilm에 대한 광역동 치료의 최적조건에 관한 연구", 대한소아치과학회지, 42(2), pp.151~157 (2015), 대한소아치과학회[DOI]
- de Carvalho FG, "Presence of mutans streptococci and Candida spp. In dental plaque", dentine of carious teeth and early childhood caries, 2006, pp.51, 1024~1028
- Gong JE, "Effect of photodynamic therapy on multispecies biofilms, including streptococcus mutans, Lactobicillus casei, and candida alibicans", Photobiomodul Photomed Laser Surg, 37, pp.282~287 (2019)
- 김재용, "Antimicrobial Effect on Streptococcus mutans in Photodynamic Therapy using Different Light Source", 대한소아치과학회지, 45(1), pp.82~89 (2018), 대한소아치과학회[DOI]
- 공정은, "Susceptibility of Mutans streptococci in the Planktonic and Biofilm State to Erythrosine", 대한소아치과학회지, 46(2), pp.135~138 (2019), 대한소아치과학회[DOI]
- Henderson BW, "How does photodynamic therapy work?", Photochem Photobiol, 55, pp.145~157 (1992)
- Bolte S, "A guided tour into subcellular colocalzation analysis in light microscopy", J Microsc, 224, pp.213~232 (2006)
- Gursoy H, "Photodynamic therapy in dentistry : a literature review", Clin Oral Investig, 17, pp.1113~1125 (2013)
- Prazmo EJ, "Photodynamic therapy as a promising method used in the treatment of oral diseases", Adv Clin Exp Med, 25, pp.799~807 (2016)