Streptococcus mutans biofilm에 대한 광역동 치료의 최적조건에 관한 연구
Optimum Treatment Parameters for Photodynamic Antimicrobial Chemotherapy on Streptococcus mutans Biofilms
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초록
할로겐 광중합기를 광원으로, 치면세균막 착색제인 erythrosine을 광감각제로 사용하여 S. mutans biofilm에 대한 광역동 치료를 시행할 때의 최적조건을 알아보기 위해서 erythrosine의 농도와 광조사 시간 및 광감각제의 접촉시간에 따른 광역동 치료의 효과를 비교하였다. erythrosine의 농도를 0, 10, 20, 40, 80 μM로 변화시킨 결과 농도가 증가할수록 항균효과가 증가하는 경향을 보였으며 40 μM과 80 μM의 두 군에서 통계적 유의성이 나타났다. 광 조사 시간을 0, 5, 15, 30, 60, 75초로 변화시킨 결과 조사 시간이 길어질수록 항균효과가 증가하는 경향을 보였으며 30초, 60초, 75초의 세 군에서 통계적유의성이 나타났다. erythrosine 처리시간을 0분, 1분, 2분30초, 5분으로 변화시킨 결과 erythrosine과의 접촉시간이 늘어날수록 항균효과가 증가하는 경향을 보였으며 2분30초 군과 5분 군에서 통계적 유의성을 보였다. 이상의 결과로 광감각제로사용한 erythrosine의 농도를 20-40 μM , 광원으로 사용한 할로겐 광중합기의 광 조사시간을 30초 이상, 광감각제인erythrosine을 2분30초 이상 처리하여 사용하는 것이 치아우식 예방을 위한 광역동 치료에 효과적임을 알 수 있다.
Abstract
The purpose of this study was to evaluate the effects of Photochemotherapy using a combination of erythrosine and standard halogen dental curing lights on the viability of Streptococcus mutans in the biofilm phase. To investigate the optimum treatment parameters, the researchers controlled the concentration of erythrosine, light irradiation time and the treatment time of erythrosine. The higher concentration of erythrosine (0, 10, 20, 40, 80 M) in the presence of light irradiation created greater effects in reducing the viability of S. mutans. The results showed a statistically significant difference among the antimicrobial effects in 20, 40, 80 M erythrosine. The higher irradiation time of light (0, 5, 15, 30, 60, 75s) in the presence of erythrosine showed greater effects in reducing the viability of S. mutans. There was statistically significant difference in 30, 60, 75 seconds. The higher treatment time of erythrosine (0, 1, 2.5, 5min) in the presence of erythrosine created greater effects on reduction of S. mutans viability. Statistically significant differences were found between 2.5 and 5 minutes of erythrosine treatment time. The results of this study showed that the photochemotherapy on S. mutans using erythrosine and the halogen dental curing lights conventionally used in dental clinics is effective in the condition of 20-40 M erythrosine concentration, irradiation time over 30 seconds, and erythrosine treatment time over 2.5 minutes.
참고문헌 (26)
- 권보민, "양산시 거주 14-16세 청소년의 치아우식증 상태에 대한 조사연구", 대한소아치과학회지, 41(1), pp.8~17 (2014), 대한소아치과학회[DOI]
- Loe H, "Oral hygiene in the prevention of caries and periodental disease", Int Dent J, 50, pp.129~139 (2000)
- Smith DJ, "Dental caries vaccines: prospects and concerns", Crit Rev Oral Biol Med, 13, pp.335~349 (2002)
- Marsh PD, "Microbiologic aspects of dental plague and dental caries", Dent Clin North Am, 43, pp.599~614 (1999)
- Francisco Ramos-Gomez, "소아치과의 새로운 비전 우식 없는 건강한 치아를 보존하기 위해: 소아치과에서의 CAMBRA 사용", 대한소아치과학회지, 40(1), pp.72~81 (2013), 대한소아치과학회
- Hoyle BD, "Bacterial resistance to antibiotics : the role of biofilms", Prog Drug Res, 37, pp.91~105 (1991)
- 이가영, "폴리비닐알코올 기반 고분자 불소 함유 테이프의 구강 내 불소 유리 성능 평가", 대한소아치과학회지, 40(2), pp.89~97 (2013), 대한소아치과학회[DOI]
- Hellwig E, "Systemic versus topical fluoride", Caries Res, 38, pp.258~262 (2004)
- 박종철, "Streptococcus mutans에 대한클로르헥시딘과 Erythrosine 광역동 치료의 상승효과", 대한소아치과학회지, 40(4), pp.241~246 (2013), 대한소아치과학회[DOI]
- Wesley MS, "Photodynamic therapeutics: basic principles and clinical applications", DDT, 4, pp.507~517 (1999)
- Konopka K, "Photodynamic therapy in dentistry", J Dent Res, 86, pp.694~707 (2007)
- Nikolaos S., "Photodynamic therapy in the control of oral biofilms", Periodontology, 55, pp.143~166 (2011)
- Juliana YN, "Antibacterial photodynamic therapy for dental caries : Evaluation of the photosensitizers used and light source properties", Photodiagnosis and Photodynamic Therapy, 9, pp.112~131 (2012)
- Zanin IC, "Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study", J Antimicrob Chemother, 56(56), pp.324~330 (2005)
- Chan Y, "Bactericidl effects of different laser wavelengths on periodontopathic germs in photodynamic therapy", Lasers Med Sci, 18, pp.51~55 (2003)
- Dobson J, "Sensitization of oral bacteria in biofilms to killing by light from a low-power laser", Arch Oral Biol, 37, pp.883~887 (1992)
- Giusti JS, "Antimicrobial photodynamic action on dentin using a light-emitting diode light source", Photomed Laser Surg, 26, pp.281~287 (2008)
- Costa AC, "Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode", Braz Oral Res, 24, pp.413~418 (2010)
- Lee YH, "The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit", Int J Oral Sci, 4, pp.196~201 (2012)
- Park MS, "Susceptibility of Streptococcus mutans to photodynamic therapy with erythrosine and dental halogen curing unit" (2011), Gangneung-Wonju National University
- 정지숙, "THE EFFECT OF PHOTODYNAMIC THERAPY ON THE VIABILITY OF STREPTOCOCCUS MUTANS ISOLATED FROM ORAL CAVITY", 대한소아치과학회지, 39(3), pp.233~241 (2012), 대한소아치과학회[DOI]
- Dougherty TJ, "Photodynamic therapy", J Natl Cancer Inst, 90, pp.889~905 (1998)
- Okamoto H, "Dye-mediated bactercidal effect of He-Ne laser irradiation on oral microorganisms", Lasers Surg Med, 12, pp.450~458 (1992)
- Marsh PD, "Antibacterial activity of some plaque-disclosing agents and dyes", Caries Res, 23, pp.348~350 (1989)
- Metcalf D, "Enhancement of erythrosine-mediated photodynamic therapy of Streptococcous mutans biofilms by light fractionation", J Antimicrob Chemother, 58, pp.190~192 (2006)
- Wood S, "Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms", J Antimicrob Chemother, 57, pp.680~684 (2006)