Antimicrobial Effect on Streptococcus mutans in Photodynamic Therapy using Different Light Source
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In a photodynamic therapy, the difference of antibacterial capacity was compared according to the type of source of light when the same quantity of energy is irradiated. After S. mutans is formed in planktonic state and biofilm state, erythrosine diluted to 40 μM was treated for 3 minutes, and as the type of light source, Halogen, LED, and Plasma arc were used, which were irradiated for 30 seconds, 15 seconds and 9.5 seconds, respectively. After the completion of the experiment, CFU of each experiment arm was measured to compare the photodynamic therapeutic effects according to each condition. The CFU of each experiment arm had no statistically significant difference. Under the same quantity of energy, the photodynamic therapeutic effect can be said to be the same regardless of types of light source, which is a useful result in the clinical field with various light irradiators.
Abstract
In a photodynamic therapy, the difference of antibacterial capacity was compared according to the type of source of light when the same quantity of energy is irradiated. After S. mutans is formed in planktonic state and biofilm state, erythrosine diluted to 40 μM was treated for 3 minutes, and as the type of light source, Halogen, LED, and Plasma arc were used, which were irradiated for 30 seconds, 15 seconds and 9.5 seconds, respectively. After the completion of the experiment, CFU of each experiment arm was measured to compare the photodynamic therapeutic effects according to each condition. The CFU of each experiment arm had no statistically significant difference. Under the same quantity of energy, the photodynamic therapeutic effect can be said to be the same regardless of types of light source, which is a useful result in the clinical field with various light irradiators.
참고문헌 (30)
- Smith DJ, "Dental caries vaccines: prospects and concerns", Crit Rev Oral Biol Med, 13, pp.335~349 (2002)[DOI]
- Marsh PD, "Microbiologic aspects of dental plaque and dental caries", Dent Clin North Am, 43, pp.599~614 (1999)
- Loe H, "Oral hygiene in the prevention of caries and periodontal disease", Int Dent J, 50, pp.129~139 (2000)[DOI]
- Hoyle BD, "Bacterial resistance to antibiotics: the role of biofilms", Prog Drug Res, 37, pp.91~105 (1991)
- Paschoal MA, "Photodynamic antimicrobial chemotherapy on Streptococcus mutans using curcumin and toluidine blue activated by a novel LED device", Lasers Med Sci, 30, pp.885~890 (2015)[DOI]
- Dougherty TJ, "An update on photodynamic therapy applications", J Clin Laser Med Surg, 20, pp.3~7 (2002)[DOI]
- Konopka K, "Photodynamic therapy in dentistry", J Dent Res, 86, pp.694~707 (2007)[DOI]
- Yildirim C, "Antimicrobial efficiency of photodynamic therapy with different irradiation durations", Eur J Dent, 7, pp.469~473 (2013)[DOI]
- Diniz IM, "Antimicrobial photodynamic therapy: a promise candidate for caries lesions treatment", Photodiagnosis Photodyn Ther, 12, pp.511~518 (2015)[DOI]
- Quishida CC, "Photodynamic inactivation of a multispecies biofilm using Photodithazine((R)) and LED light after one and three successive applications", Lasers Med Sci, 30, pp.2303~2312 (2015)[DOI]
- Ricatto LG, "Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study", Eur J Dent, 8, pp.509~514 (2014)[DOI]
- Wood S, "Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms", J Antimicrob Chemother, 57, pp.680~684 (2006)[DOI]
- Lee YH, "The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit", Int J Oral Sci, 4, pp.196~201 (2012)[DOI]
- Chui C, "Antimicrobial effect of photodynamic therapy using high-power blue light-emitting diode and red-dye agent on Porphyromonas gingivalis", J Periodontal Res, 48, pp.696~705 (2013)
- Wilson BC, "The physics, biophysics and technology of photodynamic therapy", Phys Med Biol, 53, pp.R61~R109 (2008)[DOI]
- Sharman WM, "Photodynamic therapeutics: basic principles and clinical applications", Drug Discov Today, 4, pp.507~517 (1999)[DOI]
- Soukos NS, "Photodynamic therapy in the control of oral biofilms", Periodontol 2000, 55, pp.143~166 (2011)[DOI]
- Nagata JY, "Antibacterial photodynamic therapy for dental caries: evaluation of the photosensitizers used and light source properties", Photodiagnosis Photodyn Ther, 9, pp.122~131 (2012)[DOI]
- Dougherty TJ, "Photodynamic therapy", J Natl Cancer Inst Monogr, 90, pp.889~905 (1998)[DOI]
- 최서정, "Streptococcus mutans biofilm에 대한 광역동 치료의 최적조건에 관한 연구", 대한소아치과학회지, 42(2), pp.151~157 (2015), 대한소아치과학회[DOI]
- Zanin IC, "Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study", J Antimicrob Chemother, 56, pp.324~330 (2005)[DOI]
- Hope CK, "Induction of lethal photosensitization in biofilms using a confocal scanning laser as the excitation source", J Antimicrob Chemother, 57, pp.1227~1230 (2006)[DOI]
- Araujo PV, "In vitro lethal photosensitization of Streptococcus mutans using methylene blue and toluidine blue O as photosensitizers", Acta Odontol Latinoam, 22, pp.93~97 (2009)
- Fracalossi C, "Singlet oxygen production by combining erythrosine and halogen light for photodynamic inactivation of Streptococcus mutans", Photodiagnosis Photodyn Ther, 15, pp.127~132 (2016)[DOI]
- Nomoto R, "Comparison of halogen, plasma and LED curing units", Oper Dent, 29, pp.287~294 (2004)
- 정지숙, "THE EFFECT OF PHOTODYNAMIC THERAPY ON THE VIABILITY OF STREPTOCOCCUS MUTANS ISOLATED FROM ORAL CAVITY", 대한소아치과학회지, 39(3), pp.233~241 (2012), 대한소아치과학회[DOI]
- Salva KA, "Photodynamic therapy: unapproved uses, dosages, or indications", Clin Dermatol, 20, pp.571~581 (2002)[DOI]
- Goulart Rde C, "Photodynamic therapy in planktonic and biofilm cultures of Aggregatibacter actinomycetemcomitans", Photomed Laser Surg, 28(Suppl 1), pp.S53~S60 (2010)
- Street CN, "Energy dose parameters affect antimicrobial photodynamic therapy-mediated eradication of periopathogenic biofilm and planktonic cultures", Photomed Laser Surg, 28(Suppl 1), pp.S61~S66 (2010)
- Costerton JW, "Bacterial biofilms: a common cause of persistent infections", Science, 284, pp.1318~1322 (1999)[DOI]