미국 USDA에서 도입한 수박 유전자원의 특성
Characterization of Watermelon (Citrullus Lanatus) Accessions Introduced from USDA
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초록
In this study, watermelon accessions introduced from USDA were characterized for 12 morphological characteristics of leaf, flower, fruit, and seed, according to the National Agrobiodiversity Center descriptor list. In addition, to confirm the genetic composition of each watermelon accession, genetic profiling was performed using 20 SSR markers. A total of 154 watermelon accessions from USDA were collected from 31 different countries, among them, 12.3% (19 accessions) was from Turkey. According to the results of morphological characterization performed, the accessions displayed a large diversity. In the results of genetic profiling using 20 SSR markers, six accessions (K192117, K192028, K193400, K192376, K192499, and K192800) were expected to be as the inbred lines among 154 watermelon accessions. The 12 scored characters were analyzed by discriminant analysis of principal components (DAPC). The result of DAPC revealed 68.1% of the total variability and pointed out variations among accessions, mainly on the basis of fruit stripes and fruit shape in longitudinal section. The accessions were divided into eight different groups according to DAPC, while the geographical origin was not a great source for grouping. Our results will be helpful for the efficient management of germplasms and in understanding and selecting the watermelon breeding process.
Abstract
In this study, watermelon accessions introduced from USDA were characterized for 12 morphological characteristics of leaf, flower, fruit, and seed, according to the National Agrobiodiversity Center descriptor list. In addition, to confirm the genetic composition of each watermelon accession, genetic profiling was performed using 20 SSR markers. A total of 154 watermelon accessions from USDA were collected from 31 different countries, among them, 12.3% (19 accessions) was from Turkey. According to the results of morphological characterization performed, the accessions displayed a large diversity. In the results of genetic profiling using 20 SSR markers, six accessions (K192117, K192028, K193400, K192376, K192499, and K192800) were expected to be as the inbred lines among 154 watermelon accessions. The 12 scored characters were analyzed by discriminant analysis of principal components (DAPC). The result of DAPC revealed 68.1% of the total variability and pointed out variations among accessions, mainly on the basis of fruit stripes and fruit shape in longitudinal section. The accessions were divided into eight different groups according to DAPC, while the geographical origin was not a great source for grouping. Our results will be helpful for the efficient management of germplasms and in understanding and selecting the watermelon breeding process.
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