본문 바로가기
KERD
논문KCI 등재

石斛, 石榴의 항산화, 항염증, 주름, 미백에 미치는 영향

Effects of Dendrobii herba and Punica granatum Extract on the Anti-oxidant, Anti-inflammatory, Anti-wrinkle and Whitening

서형식|노석선|황보민
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2010.12|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제23권 제3호|pp. 11-32

1

조회

0

다운로드

13

인용

0

참고문헌

키워드

skin diseasetyrosinaseMAP kinaseanti-inflammatoryelastaseendrobii herba extractPunica granatum extractskin beautycollargenase
주제분류한의학

초록

Objective : The aim of this study is to determine the effects of Dendrobii herba extract and Punica granatum extract on skin disease and skin beauty. Methods : To investigate in vitro anti-oxidant activity assay, ethanol extracts of medicinal plants tested by DPPH radical, xanthine oxidase activity. In the next experiment, to investigate anti-inflammatory activity assay, examined by relations in NO synthesis, IL-1β, IL-6, TNF-α, NF-κB, COX-2, MAP kinase. To study Skin wrinkle formation effect, we were examined by tyrosinase activities, melanin synthesis in MNT-1 cell. Results : 1. In an anti-oxidant test, Dendrobii and Punica granatum extract showed high radical scavenging activity. 2. In an anti-inflammatory test, Dendrobii herba and Punica granatum extract weakly inhibited the lipopolysaccharide(LPS)-induced nitric oxide(NO) release from RAW 246.7 macrophage cells. Dendrobii herba and Punica granatum extract also inhibited LPS-induced IL-1β and COX-2 expressions. The inhibitory effect of Dendrobii herba and Punica granatum extract on macrophage activation were via the inhibition of NF-κB, evidenced by transient transfection assay. however, Dendrobii herba and Punica granatum extract did not have any effects about activation of Jun-N-terminal kinase(JNK) and inhibition of p38 MAP kinase in RAW 264.7 cells. 3. In the skin wrinkle formation assay, Dendrobii herba and Punica granatum extract weakly inhibited collagenase and elastase, however it was not statistically significant. 4. In the skin whitening assay, Dendrobii herba and Punica granatum extract weakly inhibited tyrosinase activity, however, it was not statistically significant. They did not have any effect on melanin synthesis, indicating that they could not be applicable for skin whitening. Conclusion : Dendrobii herba extract and Punica granatum extract may play a significant role in skin disease and skin beauty.

Abstract

Objective : The aim of this study is to determine the effects of Dendrobii herba extract and Punica granatum extract on skin disease and skin beauty. Methods : To investigate in vitro anti-oxidant activity assay, ethanol extracts of medicinal plants tested by DPPH radical, xanthine oxidase activity. In the next experiment, to investigate anti-inflammatory activity assay, examined by relations in NO synthesis, IL-1β, IL-6, TNF-α, NF-κB, COX-2, MAP kinase. To study Skin wrinkle formation effect, we were examined by tyrosinase activities, melanin synthesis in MNT-1 cell. Results : 1. In an anti-oxidant test, Dendrobii and Punica granatum extract showed high radical scavenging activity. 2. In an anti-inflammatory test, Dendrobii herba and Punica granatum extract weakly inhibited the lipopolysaccharide(LPS)-induced nitric oxide(NO) release from RAW 246.7 macrophage cells. Dendrobii herba and Punica granatum extract also inhibited LPS-induced IL-1β and COX-2 expressions. The inhibitory effect of Dendrobii herba and Punica granatum extract on macrophage activation were via the inhibition of NF-κB, evidenced by transient transfection assay. however, Dendrobii herba and Punica granatum extract did not have any effects about activation of Jun-N-terminal kinase(JNK) and inhibition of p38 MAP kinase in RAW 264.7 cells. 3. In the skin wrinkle formation assay, Dendrobii herba and Punica granatum extract weakly inhibited collagenase and elastase, however it was not statistically significant. 4. In the skin whitening assay, Dendrobii herba and Punica granatum extract weakly inhibited tyrosinase activity, however, it was not statistically significant. They did not have any effect on melanin synthesis, indicating that they could not be applicable for skin whitening. Conclusion : Dendrobii herba extract and Punica granatum extract may play a significant role in skin disease and skin beauty.

참고문헌 (64)

  1. 대한피부과학회 교과서편찬위원회, "皮膚科學 개정4판", pp.45~ (2000), 麗文閣
  2. 박광균, "구강생화학", pp.318~325 (1999), 군자출판사
  3. Ehab E. Tuppo, "The role of inflammation in Alzheimer's disease", The internatinal Journal of Biochemistry & Cell Biology, 37, pp.289~305 (2004)
  4. 盧石善, "原色皮膚科學", pp.111~739 (2006), 아이비씨기획
  5. Chung JH, "Photoaging in Asians", Photodermatol Photoimmunol Photomed., 19, pp.109~121 (2003)
  6. 정진호, "미용관련 기능성 실험", pp.821~858 (2003), 건강기능식품 시험가이드 한국보건공정서연구회
  7. Deyama T, "Constituents and pharmacological effects of Eucommia and Siberian ginseng", Acta Pharmacol Sin, 22, pp.1057~1070 (2001)
  8. Randall, V. A, "Androgen and human hair growth", Clin. Endoclinol., 40, pp.439~457 (1994)
  9. Russel D.W, "Steroid 5α-reductase. two genes", two enzymes, 1993, pp.63, 25~61
  10. Rhodes L, "The effects of finasteride on hair growth, hair cycle, and serum testosterone and dihydrotestosterone in adult male and female stumptailed macaques (Mocaca arctoides)", J.Clin.Endocrinol.Metab, 79, pp.991~996 (1994)
  11. 조재훈, "鹽 醋 香油의 미백효과에 관한 연구", 대한안이비인후피부과학회지, 16(2), pp.79~95 (2003)
  12. Jung JH, "Photoaging", Photodermatol Photoimmunol Photomed, 19, pp.109~121 (2003)
  13. 原案徵中醫學院, "中醫臨床手冊", pp.1~4 (1983), 성보사
  14. 全國韓醫科大學 本草學敎室, "本草學", pp.650~682 (1995), 영림사
  15. 한홍준, "석곡이 난소적출로 골다공증이 유발된 흰쥐에 미치는 영향", 대한한방부인과학회지, 13(2), pp.120~135 (2000)
  16. 김영균, "石斛의 항산화 효과", 대한본초학회지, 20(4), pp.53~60 (2005), 대한본초학회
  17. Mizue O, "Active-oxygen scavenging activity of traditional nourishing-tonic heral medicines and active constituents of Rhodiola sacra", Journal of Ethnopharmacology, 67(1), pp.1111~1119 (1999)
  18. 최원균, "이란산 흑석류 농축액과 그 제품의 성분 및 함유된 Phyto 에스트로겐퓨에 관한 연구", 한국식품영양학회지, 15(2), pp.119~125 (2002), 한국식품영양학회
  19. 손종연, "석류씨 추출물의 항산화 및 항균활성", 한국식품조리과학회지, 21(2), pp.171~179 (2005), 한국식품조리과학회
  20. 심선미, "석류 추출서운이 암세포 증식 억제와 Quinone Reductase 유도활성에 미치는 효과", 한국식품영양과학회지, 30(1), pp.80~85 (2001)
  21. 한복진, "장내 균총조절에 유효한 소재의 탐색 및 목통, 석류, 치커리의 평가" (1995), 한양대학교 대학원 식품영양학과
  22. 노보경, "석류 추출물의 항산화 및 항노화 작용" (2005), 중앙대학교 대학원 향장품학과
  23. Blos, M.S, "Antioxidant determination by the use of stable free radical", Nature, 191, pp.1199~ (1958)
  24. Sweeney AP, "Xanthine oxidase inhibitory activity of selected Australian native plants", J Ethnopharmacol, 75, pp.273~277 (2001)
  25. Wadsworth TL, "Effects of Ginkgo biloba extract and quercetin on lipopolysaccharide-induced release of nitric oxide", Chem. Biol. Interact, 137, pp.43~58 (2001)
  26. Hinz B, "Flurbiprofen enantiomers inhibit inducible nitric oxide synthase expression in RAW264.7 macrophages", Pharm.Res., 18, pp.151~156 (2001)
  27. Chomczynski P, "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction", Anal Biochem, 162, pp.156~159 (1987)
  28. 노민수, "리포폴리사카라이드에 의해 유도되는 대식 세포의 프로스타글란딘 생합성을 저해하는 천연물의 탐색", 생약학회지, 42, pp.558~566 (1998)
  29. Kim SN, "Midazolam inhibits proinflammatory mediators in the lipopolysaccharideactivated macrophage", Anesthesiology, 105, pp.105~110 (2006)
  30. 강준, "봉약침액과 Melittin에 RAW 264.7細胞의 NO, iNOS 및 MAPK에 미치는 영향", 대한침구학회지, 21(3), pp.107~119 (1994)
  31. Beekman B, "Convenient fluorometric assay for matrix metalloproteinase activity and its application in biological media", FEBS Lett, 390, pp.221~225 (1996)
  32. 부희정, "감태(Ecklonia cava)에서 분리한 Phlorotannin 성분의Elastase 저해활성 및 Hyaluronidase 저해활성", 생약학회지, 37(2), pp.92~96 (2006), 한국생약학회
  33. Roh JS, "Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) seeds for melanogenesis", Biol Pharm Bull, 27, pp.1976~1978 (2004)
  34. Shrayer DP, "The effect of Taurolidine on adherent and floating subpopulations of melanoma cells", Anticancer Drugs, 14, pp.295~303 (2003)
  35. Denizot F, "Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability", Immunol.Methods, 89, pp.271~277 (1986)
  36. 대한병리학회, "병리학", pp.71~104 (1995), 고문사
  37. 陳實功, "外科正宗", pp.47~ (1993), 天津科學技術出版社
  38. 楊維傑, "黃帝內經解釋", pp.147~456 (1997), 成輔社
  39. 華 陀, "中藏經:劉河間三六書", pp.82~ (1976), 成輔社
  40. 王 燾, "外臺秘要", pp.107~631 (1982), 人民衛生出版社
  41. 薛 己, "薛己醫案", pp.349~355 (1976), 成輔社
  42. 嚴用和, "濟生方", pp.61~68 (1965), 東洋醫藥大學
  43. 危亦林, "得效方", pp.613~ (1965), 東洋醫藥大學
  44. 김륭규, "넘취 정유의 Murine Macrophage Raw 264.7세포에서 In vitro 항염효과", 약 학 회 지, 46(5), pp.343~347 (2002), 대한약학회
  45. 서영준, "cyclooxigenase-2의 역할 및 그 저해를 통한 화학 암예방", 분자세포생물학뉴스, 13, pp.8~17 (2002)
  46. Lee B.G, "Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophage by two β-carboline alkaloidsextracted from Melia azedarach", European J. Pharmacol, 406, pp.301~309 (2000)
  47. Kim E.J, "Suppression by a sesquiterpene lactone from Carpesium divaricantum of inducible nitric oxide synthase by inhibiting nuclear factor-κB activation", Biochem. Pharmarol, 61, pp.903~910 (2001)
  48. Aeberi D, "Inhibition of the TNF-pathway:use of infliximab an etanercept as remissioninducing agents in case of therapy-resistant chronic inflammatory disorders", Swiss Med. Wkly, 132, pp.414~422 (2002)
  49. 오세관, "키워드로 해석하는 세포신호전달 일러스트", pp.21~31 (2006), 월드사이언스
  50. 문태철, "천연물로부터 COX-2 저해제 검색", 약학회지, 42, pp.214~219 (1998)
  51. 일본 생화학회, "세포운명과 세포기능을 조절하는 구조 정보 전달 제6장 MAP 카아나제", pp.93~104 (2006), 월드사이언스
  52. 김경동, "한방 천연식물의 항산화 및 자외선 흡수에 관한 연구", 한국미용학회지, 10(2), pp.145~153 (2004)
  53. 강원형, "피부질환 아틀라스", pp.8~384 (2003), 한미의학
  54. Jung JH, "Photoaging", Photodermatol Photoimmunol Photomed, 19, pp.109~121 (2003)
  55. 程士德, "素問注釋匯粹", pp.395~396 (1982), 人民衛生出版社
  56. 李 梴, "編註醫學入門", pp.29~224 (1990), 대성문화사
  57. 趙 佶, "聖濟總錄", pp.1763~ (1987), 人民衛生出版社
  58. 許 浚, "東醫寶鑑", pp.97~309 (1990), 대성문화사
  59. 祁 坤, "外科大成", pp.211~212 (1980), 文光圖書有限公司
  60. 박호순, "가미귀비탕의 미백효과에 관한 연구", 대한안이비인후피부과학회지, 17(2), pp.48~58 (2004)
  61. Pawelek JM, "The biosynthesis of mammalian melanin", Am Sci, 70, pp.136~145 (1982)
  62. Kobayashi T, "DHICA oxidase activity of TRP 1 and interactions with other melanogenic enzymes", Pigment Cell Res, 7, pp.227~234 (1994)
  63. Korner AM, "Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin", Science, 217, pp.1163~1165 (1982)
  64. Seiji M, "Subcellular localization of melanin biosynthesis", Ann NY Acad Sci, 100, pp.497~533 (1963)

관련 논문AI추천

석류잎 추출액의 항산화 작용과 미백작용이동자, 허정록대한미용학회 · 대한미용학회지2012 · 제8권 제4호蘆薈(알로에), 紫花地丁의 항산화, 항염증, 주름, 미백에 미치는 영향김창훈, 노석선, 정현아, 홍석훈대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2010 · 제23권 제1호한국 자생 석곡 메탄올 추출물의 미백 및 주름개선 효과강병만, 고재형, 김성철, 빈철구, 여준환, 장지훈, 정원석, 정호경, 조정희, 조현우한국약용작물학회 · 한국약용작물학회지2014 · 제22권 제5호平肝開鬱止血湯의 항염증, 항산화 및 항노화에 미치는 영향김희택, 송영채, 이경원대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2024 · 제37권 제1호대분청음(大分淸飮)추출물의 항염증 및 항산화 효과김용민, 김이화, 박세근, 원혜련대한미용학회 · 대한미용학회지2019 · 제15권 제4호丹皮湯 추출물의 항산화 및 항염증 효과 연구김용민, 김이화, 박혜수, 오솔라대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2019 · 제32권 제3호

이 간행물의 다른 논문 (16건)

普濟消毒飮加減方의 消炎作用에 관한 實驗的 硏究박종형, 김성학, 김경준pp. 1-10數種 한약재의 콜라겐 생합성 효과에 대한 실험적 연구박민철, 김남권, 황충연, 윤지은, 조은희pp. 33-41화피 추출물이 염증 및 알레르기 반응에 미치는 영향정현아, 정지영pp. 42-65강원지역 소재 한방병원 피부과 외래환자의 임상적 분석권강, 김지은, 김지현, 서형식, 유영진pp. 66-77灸法과 intense pulsed light(IPL)의 특성 비교 및 光線灸의 韓方臨床 活用선승호, 남동현, 금수연, 장인수, 서형식pp. 78-83
원문 보기