한해성 품종 종묘생산을 위한 로티퍼의 적정 영양강화 수온 및 미세조류 선택
Optimal Enrichment Temperature for Rotifer and Microalgae Selection for Cold-Water Species Culture
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The purpose of this study is to establish methods for enhancing the survival and growth of cold-water fish and crustacean larvae based on the nutritional components of zoo and phyto live foods. Rotifers, Brachionus rotundiformis, were cultured with a supplement of freshwater condensed Chlorella vulgaris at 28℃ and enriched with Algamac 2000® at 16, 20 and 26℃, respectively. Isochrysis galbana, Chaetoceros calcitrans and Chlorella ellipsoidea were centrifuged for component analysis after being cultured for approximately one week with conway medium at 20℃. The crude protein and lipid contents of the rotifers were 58.4% and 10.9%, respectively, before enrichment. After enrichment at each temperature, total protein and essential amino acid contents were increased by reducing the enrichment temperature. However, unsaturated fatty acids and multiple fatty acid index (UI) showed their highest values at 20℃. Mono-unsaturated fatty acid content was highest (72.6%) at 16℃. The total protein contents of C. calcitrans and C. ellipsoidea were higher, 33.0% and 35.2%, respectively, than that of I. galbana, 27.8%. Methionin, leusine and histidine, essential amino acids of C. ellipsoidea , had considerably higher values, 50.2, 287.2 and 68.1 mg/g dry matter, respectively, compared to other microalgae. Total lipids, UI, DHA and n-3 PUFA of I. galbana had higher values, 23.6, 272.0, 12.9% and 45.2%, respectively, than other microalgae. Therefore, for cold-water fish and crustacean larvae that require high n-3 PUFA and DHA contents, enrichment of rotifers is desirable at 20℃. Fish larvae would also need more I. galbana than other microalgae.
Abstract
The purpose of this study is to establish methods for enhancing the survival and growth of cold-water fish and crustacean larvae based on the nutritional components of zoo and phyto live foods. Rotifers, Brachionus rotundiformis, were cultured with a supplement of freshwater condensed Chlorella vulgaris at 28℃ and enriched with Algamac 2000® at 16, 20 and 26℃, respectively. Isochrysis galbana, Chaetoceros calcitrans and Chlorella ellipsoidea were centrifuged for component analysis after being cultured for approximately one week with conway medium at 20℃. The crude protein and lipid contents of the rotifers were 58.4% and 10.9%, respectively, before enrichment. After enrichment at each temperature, total protein and essential amino acid contents were increased by reducing the enrichment temperature. However, unsaturated fatty acids and multiple fatty acid index (UI) showed their highest values at 20℃. Mono-unsaturated fatty acid content was highest (72.6%) at 16℃. The total protein contents of C. calcitrans and C. ellipsoidea were higher, 33.0% and 35.2%, respectively, than that of I. galbana, 27.8%. Methionin, leusine and histidine, essential amino acids of C. ellipsoidea , had considerably higher values, 50.2, 287.2 and 68.1 mg/g dry matter, respectively, compared to other microalgae. Total lipids, UI, DHA and n-3 PUFA of I. galbana had higher values, 23.6, 272.0, 12.9% and 45.2%, respectively, than other microalgae. Therefore, for cold-water fish and crustacean larvae that require high n-3 PUFA and DHA contents, enrichment of rotifers is desirable at 20℃. Fish larvae would also need more I. galbana than other microalgae.
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