고온기 배양에 적합한 Isochrysis 와 Pavlova 종의 선정
Selection of Isochrysis and Pavlova Species for Mass Culture in High Temperature Season
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Even though the microalgal species of Isochrysis and Pavlova are widely used as live food in bivalve hatcheries, they are difficult to culture in mass during the summer season. Therefore, the present study was conducted to determine the optimum species or strains of Isochrysis and Pavlova to produce good growth and high contents of fatty acids at temperatures over 30°C. Four species of Isochrysis (I. galbana KMMCC12, I. galbana KMMCC214, I. aff. galbana, and Isochrysis sp.) and four of Pavlova (P. lutheri, P. gyrans, P. viridis, and Pavlova sp.) were cultured at 25°C, 29°C, and 33°C, and then analyzed for specific growth rate and fatty acid composition. Microalgae were cultured in f/2 medium at 23 psu and continuous light of 80 μmol photons m-2 s-1. For the Isochrysis species, growth rates were highest at 29°C and decreased at 33°C to the level observed at 25°C. Isochrysis galbana (KMMCC12) and Isochrysis sp. cultured at 29°C and 33°C, respectively, exhibited the highest growth rates of all Isochrysis species. In terms of fatty acids, I. galbana (KMMCC12) contained higher contents of PUFA and n-3 HUFA at 33°C than did Isochrysis sp. For species of Pavlova,growth rates of P. gyrans and P. viridis at 29°C and 33°C, respectively, were higher than those of the other Pavlova species. In particular, P. viridis grew as well at 33°C as it did at 29°C. However, P. lutheri and Pavlova sp. did not grow at 33°C. In terms of fatty acids, P. viridis cultured at 33°C also exhibited higher contents of PUFA and n-3 HUFA, as compared to P. gyrans. Based on these results, we suggest that I. galbana (KMMCC12) and P. viridis are suitable species for mass culture during the high temperature season.
Abstract
Even though the microalgal species of Isochrysis and Pavlova are widely used as live food in bivalve hatcheries, they are difficult to culture in mass during the summer season. Therefore, the present study was conducted to determine the optimum species or strains of Isochrysis and Pavlova to produce good growth and high contents of fatty acids at temperatures over 30°C. Four species of Isochrysis (I. galbana KMMCC12, I. galbana KMMCC214, I. aff. galbana, and Isochrysis sp.) and four of Pavlova (P. lutheri, P. gyrans, P. viridis, and Pavlova sp.) were cultured at 25°C, 29°C, and 33°C, and then analyzed for specific growth rate and fatty acid composition. Microalgae were cultured in f/2 medium at 23 psu and continuous light of 80 μmol photons m-2 s-1. For the Isochrysis species, growth rates were highest at 29°C and decreased at 33°C to the level observed at 25°C. Isochrysis galbana (KMMCC12) and Isochrysis sp. cultured at 29°C and 33°C, respectively, exhibited the highest growth rates of all Isochrysis species. In terms of fatty acids, I. galbana (KMMCC12) contained higher contents of PUFA and n-3 HUFA at 33°C than did Isochrysis sp. For species of Pavlova,growth rates of P. gyrans and P. viridis at 29°C and 33°C, respectively, were higher than those of the other Pavlova species. In particular, P. viridis grew as well at 33°C as it did at 29°C. However, P. lutheri and Pavlova sp. did not grow at 33°C. In terms of fatty acids, P. viridis cultured at 33°C also exhibited higher contents of PUFA and n-3 HUFA, as compared to P. gyrans. Based on these results, we suggest that I. galbana (KMMCC12) and P. viridis are suitable species for mass culture during the high temperature season.
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