Effect of Sodium Citrate on Growth of Bacteria in Blood Culture
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Background: This study compared the growth of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Haemophilus influenzae in blood culture bottles containing anticoagulants, sodium polyanethol sulfonate (SPS)and sodium citrate. Methods: One hundred and fifty colony forming units of five different bacterial species were inoculated into standard aerobic (SA) and standard anaerobic (SN)bottles and were combined with 5 mL of human blood in solution with SPS or sodium citrate. Time to detection (TTD) was then monitored using the BacT/Alert 3D system (bioMerieux Inc.). Results: Compared to the bacteria-only controls, cultures containing S. aureus, E. coli, P. aeruginosa,and S. pneumoniae plus SPS blood or citrated blood trended toward reduced TTD in both SA and SN bottles;however, there was no significant difference in TTD between SPS and sodium citrate anticoagulant. Although H. influenzae showed a remarkable difference in TTD between SPS (SA 14.8 h, SN 15.0 h)and sodium citrate (SA 23.5 h, SN 18.3 h), this difference was not statistically significant (P=0.10). Conclusion: Addition of blood enhanced growth of bacteria. All experimental bacteria except H. influenzae showed similar TTD in SPS blood and citrated blood. These results support the usefulness of sodium citrate anticoagulant for artificial inoculation in blood culture bottles.
Abstract
Background: This study compared the growth of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Haemophilus influenzae in blood culture bottles containing anticoagulants, sodium polyanethol sulfonate (SPS)and sodium citrate. Methods: One hundred and fifty colony forming units of five different bacterial species were inoculated into standard aerobic (SA) and standard anaerobic (SN)bottles and were combined with 5 mL of human blood in solution with SPS or sodium citrate. Time to detection (TTD) was then monitored using the BacT/Alert 3D system (bioMerieux Inc.). Results: Compared to the bacteria-only controls, cultures containing S. aureus, E. coli, P. aeruginosa,and S. pneumoniae plus SPS blood or citrated blood trended toward reduced TTD in both SA and SN bottles;however, there was no significant difference in TTD between SPS and sodium citrate anticoagulant. Although H. influenzae showed a remarkable difference in TTD between SPS (SA 14.8 h, SN 15.0 h)and sodium citrate (SA 23.5 h, SN 18.3 h), this difference was not statistically significant (P=0.10). Conclusion: Addition of blood enhanced growth of bacteria. All experimental bacteria except H. influenzae showed similar TTD in SPS blood and citrated blood. These results support the usefulness of sodium citrate anticoagulant for artificial inoculation in blood culture bottles.
참고문헌 (9)
- Wilson ML, "Principles and procedures for blood cultures; approved guideline" (2007), Clinical and Laboratory Standards Institute
- Thomson R, "Specimen Collection, Transport and Pro- Cessing: Bacteriology, In Manual of Clinical Microbiology. Vol. 1", pp.286~330 (2003), ASM Press
- Reimer LG, "Update on detection of bacteremia and fungemia", Clin Microbiol Rev, 10, pp.444~465 (1997)
- 김희정, "Characteristics of Microorganisms Isolated from Blood Cultures at Nine University Hospitals in Korea during 2009", Annals of Clinical Microbiology, 14(2), pp.48~54 (2011), 대한임상미생물학회
- Sautter RL, "Effects of delayed-entry conditions on the recovery and detection of microorganisms from BacT", ALERT and BACTEC blood culture bottles, 2006, pp.44, 1245~1249
- Chapin K, "Comparison of Bactec 9240 and Difco ESP blood culture systems for detection of organisms from vials whose entry was delayed", J Clin Microbiol, 34, pp.543~549 (1996)
- Viganò EF, "Use of simulated blood cultures for time to detection comparison between BacT", ALERT and BACTEC 9240 blood culture systems, 2002, pp.44, 235~240
- Akan OA, "Comparison of the effect of delayed entry into 2 different blood culture systems (BACTEC 9240 and BacT", ALERT 3D) on culture positivity, 2006, pp.54, 193~196
- Klaerner HG, "Failure of an automated blood culture system to detect nonfermentative gram-negative bacteria", J Clin Microbiol, 38, pp.1036~1041 (2000)