Maroon clownfish, Premnas biaculeatus 자어로부터 분리한 Vibrio ponticus KJS1의 특성
Characterization on Vibrio ponticus KJS1 Isolated from Larvae of Maroon Clownfish, Premnas biaculeatus
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비브리오증은 해수어의 중요한 질병중 하나로 알려져 있다. 본 연구에서 maroon clownfish (Premnas biaculeatus)를 대상으로 해수 관상어 종묘 생산 기술 개발 과정 중 부화 3～7일령 사이에 섭이를 하지 않고 힘없이 유영하면서 폐사하는 개체들로부터 Vibrio 균주를 분리하였다.분리균주의 16S rRNA gene 염기서열 분석결과 Vibrio ponticus CECT5869 와 99.8%의 상동성을 보여 Vibrio ponticus KJS1으로 동정하였다.GN2 microplate (BioLog, USA)를 이용한 생화학 시험결과에서 dextrin 및 sucrose를 포함한 35가지 기질 이용능이 확인되었고, α-cyclodextrin을 비롯한 52가지 기질은 분해하지 못하였으며, glycogen을 비롯한 7가지 기질은 약한 이용능을 나타내었다.분리 균주에 대한 항생제 감수성 실험 결과에서는 oxolinic acid, flumequine, ciprofloxacin, ofloxacin, norfloxacin, nalidixic acid, pefloxacin, oxycycline hydrochloride에 높은 감수성을 나타내었다.
Abstract
Vibriosis is one of the most important diseases affecting marin fish. In this study, a bacterium belonging to the Vibrio were isolated from maroon clownfish (Premnas biaculeatus) larvae (3～7 days after hatching). The bacterium was identified as Vibrio ponticus KJS1 by 16S rRNA gene sequence analysis. The result of biochemical test using the GN2 microplate (BioLog Inc, USA) showed that it has the ability to use 35 substrates including dextrin and sucrose was detected, could not degrade 52 substrates including α-cyclodextrin, and showed the weak ability to use 7 substrates including glycogen. It was sensitive to oxolinic acid, flumequine, ciprofloxacine, ofloxacin, norfloxacin, nalidixic acid, pefloxacin, and doxycycline hydrochloride.
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