山白菊 열수추출물이 RAW 264.7 대식세포에 미치는 항염증 효과
Anti-inflammatory Effects of Aster glehni Water Extracts in LPS-stimulated RAW 264.7 Macrophages
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Objectives : This study was conducted to confirm the anti-inflammatory effects of Aster glehni Water extracts. Methods : In this study, MTT assay was performed to detect cell viability. To evaluate the anti-inflammatory effects of Aster glehni Water extracts, we examined NO production in LPS-induced macrophages. Expressions of iNOS, COX-2, ERK, p38, JNK were also investigated by using western blot assay. Results : Aster glehni Water extracts have no cytotoxicity at 15.625-1,000㎍/㎖ in RAW 264.7 cells. Aster glehni Extracts inhibited the NO production in a dose-dependent manner in RAW 264.7 cells treated with LPS. Pretreated 250, 500, 1,000㎍/㎖ of Aster glehni water extracts had significantly suppressed expression levels of iNOS, COX-2, p-ERK, p-p38, p-JNK. Conclusions : These results suggest that Aster glehni Water extracts have anti-inflammatory effects and can be used for various inflammatory skin diseases.
Abstract
Objectives : This study was conducted to confirm the anti-inflammatory effects of Aster glehni Water extracts. Methods : In this study, MTT assay was performed to detect cell viability. To evaluate the anti-inflammatory effects of Aster glehni Water extracts, we examined NO production in LPS-induced macrophages. Expressions of iNOS, COX-2, ERK, p38, JNK were also investigated by using western blot assay. Results : Aster glehni Water extracts have no cytotoxicity at 15.625-1,000㎍/㎖ in RAW 264.7 cells. Aster glehni Extracts inhibited the NO production in a dose-dependent manner in RAW 264.7 cells treated with LPS. Pretreated 250, 500, 1,000㎍/㎖ of Aster glehni water extracts had significantly suppressed expression levels of iNOS, COX-2, p-ERK, p-p38, p-JNK. Conclusions : These results suggest that Aster glehni Water extracts have anti-inflammatory effects and can be used for various inflammatory skin diseases.
참고문헌 (27)
- Sung YY, "Inhibitory effects of Drynar ia fortunei extracts on house dust miteantig en-induced atopic dermatitis in NC", Nga mice, 2012, pp.144(1), 94~100
- Cho W, "Zedoarondiol isolated from the rhizo ma of Curcuma heyneana is involved in the in hibition of iNOS, COX-2 and pro-inflammatory cytokines via the downregulation of NF-κB p athway in LPS-stimulated murine macrophages", Immunopharmacol, 9(9), pp.1049~1057 (2009)
- 김동희, "대식세포 내에서의 홍화자 추출물의 항염증 활성", 생명과학회지, 23(1), pp.55~62 (2013), 한국생명과학회[DOI]
- Guzik T, "Nitric oxide and superoxide in inflammation", J Physiol Pharmacol, 54(4), pp.469~487 (2003)
- Nathan C, "Points of control in inflammation", N ature, 420(6917), pp.846~852 (2002)
- 정다현, "미역(Undaria pinnatifida) 물 추출물의 염증 억제 활성", Journal of Applied Biological Chemistry, 55(4), pp.221~225 (2012), 한국응용생명화학회
- 강보경, "Lipopolysaccharide로 유도된 RAW 264.7 세포에서 다시마 뿌리에탄올 추출물의 항염증 효과", 한국식품과학회지, 46(6), pp.729~733 (2014), 한국식품과학회[DOI]
- Jung BS, "Dohae Hyangyak Dictionary", pp.1020~1021 (2003), Yeonglimsa
- Seo SW, "Antioxidant Activities of Aster glehni Extracted with Different Solvents", Iran J Public Health, 48(1), pp.176~178 (2019)
- 김한혁, "섬쑥부쟁이(Aster glehni Fr. Schm.) 분획물의 항산화 및 항염증 활성", 한국미생물·생명공학회지, 38(4), pp.434~441 (2010), 한국미생물·생명공학회
- Lee HM, "Antiadipogenic Effects of Aster glehni Extract : In Vivo and In Vitro Effects", Evid Based Complement Alternat Med, 2013, pp.1~10 (2013)
- Agung Nugroho, "Phytochemical Studies of the Phenolic Substances in Aster glehni Extract and its Sedative and Anticonvulsant Activity", Archives of Pharmacal Research, 35(3), pp.423~430 (2012), 대한약학회
- Kundu JK, "Inflammation : gearing the journey to cancer", Mutat Res, 659(1-2), pp.15~30 (2008)
- Hwang JH, "Caffeine prevents LPS-induced inflammatory responses in RAW 264.7 cells and zebrafish", Chemico biological interaction, 248, pp.1~7 (2016)
- Arthur JS, "Mitogen-activated protein kinases in innate immunity", Nat Rev Immunol, 13(9), pp.679~692 (2013)
- Lee YS, "IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans", J Microbiol Biotechnol, 10(1), pp.8~15 (2000)
- Higuchi M, "Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages", J Immunol, 144(4), pp.1425~1431 (1990)
- 정혜림, "RAW264.7 대식세포에서 Heme Oxygenase-1 발현을 통한 배암차즈기(Salvia plebeia R. Br.) 잎 추출물의 항염증효과", 한국식품영양과학회지, 41(7), pp.888~894 (2012), 한국식품영양과학회[DOI]
- 김연숙, "RAW264.7 대식세포에서 왕쥐똥나무잎 추출물의 항염증 효과", 한국식품영양과학회지, 41(9), pp.1205~1210 (2012), 한국식품영양과학회
- Miyasaka N, "Nitric oxide and inflammatory arthritides", Life Sci, 61(21), pp.2073~2081 (1997)
- Simmons ML, "Induction of nitric oxide from glial cells", J Neurochem, 59(3), pp.897~905 (1992)
- Martel-Pelletier J, "Cyclooxygen-ase-2 and prostaglandins in articular tissues", Semin Arthritis Rheum, 33(3), pp.155~167 (2003)
- Needleman P, "The discovery and function of COX-2", J Rheumatol, 49, pp.6~8 (1997)
- Seybold VS, "Cyclo-oxygenase-2 contributes to central sensitization in rats with peripheral inflammation", Pain, 105(1-2), pp.47~55 (2003)
- Yoon JH, "p-coumaric acid and ursolic acid from Corni fructus attenuated β-amyloid(25-35)-induced toxicity through regulation of the NF-κB signaling pathway in PC12 cells", J Agric Food Chem, 62(21), pp.4911~4916 (2014)
- Kao SJ, "Lipoteichoic acid induces nuclear factor-kappaB activation and nitric oxide synthase expression via phosphatidy linositol 3-kinase, Akt, and p38 MAPK in RA W 264.7 macrophages", Immunology, 115(3), pp.366~374 (2005)
- Meyer CF, "Interaction between c-Rel and the mitogen-activated protein kinase kinase kinase 1 signaling cascade in mediating kappaBenhancer activation", J Biol Chem, 271(15), pp.8971~8976 (1996)
