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논문KCI 등재

蒲公英이 유방암 세포의 사멸에 미치는 영향

Effects of Taraxaci Herba on Cell Death in Breast Cancer Cells

조성희|양승정|박경미|서국장
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2015.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제28권 제2호|pp. 1-14|DOI http://dx.doi.org/10.15204/jkobgy.2015.28.2.001

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참고문헌

키워드

Taraxaci Herbabreast cancer cellautophagy
주제분류한의학

초록

Objectives: This study was designed to investigate the effects of Taraxaci Herba (TH) on cell death in breast cancer cells. Methods: In this experiment, the effects of TH on proliferation rates, cell morphology and growth pattern, intracellular reactive oxygen species (ROS) production. In addition, the effects on nuclear condensation, fragmentation and formation of acidic vesicular organelles (AVO) in MCF-7 cells were also investigated. Finally, autophagy related with protein was observed by using western blot method. Results: TH inhibited proliferation of MCF-7 cells, TH elevated intracellular ROS levels significantly. Treatment with TH did not affect nuclear morphologies such as condensation or fragmentation. On the other hand, TH treatment effectively induced AVO. Finally, one of autophagy related with protein, Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, LC3) level was elevated by treatment with TH. Conclusions: These data indicate that TH is able to be used for patient with breast cancer and mechanisms are involved in autophagy through ROS generation.

Abstract

Objectives: This study was designed to investigate the effects of Taraxaci Herba (TH) on cell death in breast cancer cells. Methods: In this experiment, the effects of TH on proliferation rates, cell morphology and growth pattern, intracellular reactive oxygen species (ROS) production. In addition, the effects on nuclear condensation, fragmentation and formation of acidic vesicular organelles (AVO) in MCF-7 cells were also investigated. Finally, autophagy related with protein was observed by using western blot method. Results: TH inhibited proliferation of MCF-7 cells, TH elevated intracellular ROS levels significantly. Treatment with TH did not affect nuclear morphologies such as condensation or fragmentation. On the other hand, TH treatment effectively induced AVO. Finally, one of autophagy related with protein, Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, LC3) level was elevated by treatment with TH. Conclusions: These data indicate that TH is able to be used for patient with breast cancer and mechanisms are involved in autophagy through ROS generation.

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