유산균발효애엽과 효모균발효애엽 물추출물의 종양괴사인자-알파 생성촉진효과
Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus and Saccharomyces Cerevisiae on TNF-α Production in RAW 264.7 and HepG2 Cells
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Tumor necrosis factor-alpha (TNF-α) is a major mediator of immuno-inflammatory activity. The purpose of this study is to investigate whether TNF-α productions of mouse macrophage RAW 264.7 and human hepatocyte HepG2 are modulated by Artemisiae argi Folium water extract (AW), Lactobacillus pentosus-fermented Artemisiae argi Folium water extract (AFL), and Saccharomyces cerevisiae-fermented Artemisiae argi Folium water extract (AFS) for 3 h of incubation. Effect of AW on cell viability of HepG2 was also investigated. TNF-α productions were measured by Enzyme-Linked Immnunosorbent Assay method and cell viability was measured by MTT assay. Both AFL and AFS significantly increased TNF-α productions of RAW 264.7 at the concentration of 50, 100, and 200 μg/mL (p<0.05). Also, AFL and AFS significantly increased TNF-α productions of HepG2 at the concentration of 50, 100, and 200 μg/mL (p<0.05). AW significantly increased TNF-α production of HepG2 at the concentration of 100 and 200 μg/mL (p<0.05). AW did not show any cytotoxicity on HepG2 cells for 3 h. These results suggest that AFL, AFS, and AW have the immune-enhancing property related with its increasing effect on TNF-α production of macrophage and hepatocyte.
Abstract
Tumor necrosis factor-alpha (TNF-α) is a major mediator of immuno-inflammatory activity. The purpose of this study is to investigate whether TNF-α productions of mouse macrophage RAW 264.7 and human hepatocyte HepG2 are modulated by Artemisiae argi Folium water extract (AW), Lactobacillus pentosus-fermented Artemisiae argi Folium water extract (AFL), and Saccharomyces cerevisiae-fermented Artemisiae argi Folium water extract (AFS) for 3 h of incubation. Effect of AW on cell viability of HepG2 was also investigated. TNF-α productions were measured by Enzyme-Linked Immnunosorbent Assay method and cell viability was measured by MTT assay. Both AFL and AFS significantly increased TNF-α productions of RAW 264.7 at the concentration of 50, 100, and 200 μg/mL (p<0.05). Also, AFL and AFS significantly increased TNF-α productions of HepG2 at the concentration of 50, 100, and 200 μg/mL (p<0.05). AW significantly increased TNF-α production of HepG2 at the concentration of 100 and 200 μg/mL (p<0.05). AW did not show any cytotoxicity on HepG2 cells for 3 h. These results suggest that AFL, AFS, and AW have the immune-enhancing property related with its increasing effect on TNF-α production of macrophage and hepatocyte.
참고문헌 (18)
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