애엽과 음양곽 혼합 발효물이 대식세포 활성에 미치는 영향
Fermented Artemisiae Argyi Folium and Epimedii Herba Mixture Effect on Macrophage’ Activity
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Purpose: This research aimed to study the effect of FAE(Ferment Artemisiae Argyi Folium and Epimedii Herba) on the mouse macrophage cell activity. Methods: Effect of FAE, which was fermented by Sacchromyces cerevisiae STV89, on cell viability, amount of H2O2 within cells, amount of NO was measured and compaperd by using mouse macrophage cells. Results: 1. Result of MTT assay conducted to observe the effect of FAE on the survival rate of mouse macrophage cells illustrated that, when FAE was proccessed for each concentration, there was no significant decrease of the survival rate. 2. FAE increased the amount of H2O2 within macrophage cells and increased inhibition of amount of H2O2 in macrophage induced by LPS. 3. FAE inhibited amount of NO in macrophage cells, and significantly inhibited increase of amount of NO in mcacrophage induced by LPS. Conclusion: FAE produced by Artemisiae Argyi Folium and Epimedii Herba did not induce the decrease of macrophage cell survival rate, increased amount of H2O2 within cells, and reduced amount of NO. FAE significantly increase by LPS, reduced the increase of amount of NO in macrophage induced by LPS. These results signify FAE has significant effect on immuno modulating activity of macrophage.
Abstract
Purpose: This research aimed to study the effect of FAE(Ferment Artemisiae Argyi Folium and Epimedii Herba) on the mouse macrophage cell activity. Methods: Effect of FAE, which was fermented by Sacchromyces cerevisiae STV89, on cell viability, amount of H2O2 within cells, amount of NO was measured and compaperd by using mouse macrophage cells. Results: 1. Result of MTT assay conducted to observe the effect of FAE on the survival rate of mouse macrophage cells illustrated that, when FAE was proccessed for each concentration, there was no significant decrease of the survival rate. 2. FAE increased the amount of H2O2 within macrophage cells and increased inhibition of amount of H2O2 in macrophage induced by LPS. 3. FAE inhibited amount of NO in macrophage cells, and significantly inhibited increase of amount of NO in mcacrophage induced by LPS. Conclusion: FAE produced by Artemisiae Argyi Folium and Epimedii Herba did not induce the decrease of macrophage cell survival rate, increased amount of H2O2 within cells, and reduced amount of NO. FAE significantly increase by LPS, reduced the increase of amount of NO in macrophage induced by LPS. These results signify FAE has significant effect on immuno modulating activity of macrophage.
참고문헌 (72)
- 정용준, "발효한약추출물 HP-1이 알코올을 투여한 쥐의 알코올 대사에 미치는 영향", 동의생리병리학회지, 21(2), pp.387~391 (2007), 대한동의병리학회
- 韓醫科大學 本草學 編纂委員會, "本草學", pp.405~406 (1991), 영림사
- 한효상, "艾葉 발효 추출물의 세포독성과 면역활성에 관한 연구", 박사, pp.2008
- 최용덕, "淫羊藿 水抽出物이 생쥐의 면역글로불린 및 Cytokine 생성에 미치는 영향", 동의생리병리학회지, 15(2), pp.300~310 (2001), 대한동의병리학회
- Ferrari M, "MTT colorimetric assay for testing macrophage cytotoxic activity in vitro", J Immunol Methods, 131(2), pp.165~172 (1990)
- Oez S, "A quantitative colorimetric method to evaluate the functional state of human polymorphonuclear leukocytes", Blut, 60(2), pp.97~102 (1990)
- Gerlier D, "Use of MTT colorimetric assay to measure cell activation", J Immunol Methods, 94(1-2), pp.57~63 (1986)
- Roesler J, "Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry", Eur J Pediatr, 150(3), pp.161~165 (1991)
- Crow JP, "Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species", Nitric Oxide, 1(2), pp.145~157 (1997)
- Van Pelt LJ, "Limitations on the use of dihydrorhodamine 123 for flow cytometric analysis of the neutrophil respiratory burst", J Immunol Methods, 191(2), pp.187~196 (1996)
- Jirapongsananuruk O, "Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay", J Allergy Clin Immunol, 111(2), pp.374~379 (2003)
- Richardson MP, "A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test", J Immunol Methods, 219(1-2), pp.187~193 (1998)
- Weissman BA, "Measurement of NO and NO synthase", Curr Protoc Neurosci, 7(7), pp.13~ (2001)
- Giustarini D, "Nitrite and nitrate measurement by Griess reagent in human plasma: evaluation of interferences and standardization", Methods Enzymol, 440, pp.361~680 (2008)
- Reher VG, "Nitric oxide levels in saliva increase with severity of chronic periodontitis", J Oral Sci, 49(4), pp.271~276 (2007)
- Casanova JA, "Use of Griess reagent containing vanadium(III) for post-column derivatization and simultaneous determination of nitrite and nitrate in baby food", J AOAC Int, 89(2), pp.447~451 (2006)
- 윤택준, "발효된 한국산 겨우살이 추출물의 Macrophage 자극에 의한 면역적 활성화와 종양전이 억제효과", 한국식품과학 학회지, 31(3), pp.838~847 (1999)
- 손미예, "버섯균사체로 발효시킨 복령과 후박의 항산화 및 항암효과", 식품산업과영양, 12(2), pp.51~57 (2007)
- 차재영, "차가버섯과 어성초 함유 발효 조성물이 인체 위암 AGS 및 대장암 HCT-15 세포 생육에 미치는 영향", 한국응용생명화학지, 47(2), pp.202~207 (2004)
- 동의보감 국역위원회, "對譯 東醫寶鑑", pp.1941~ (1999), 법인문화사
- 임은미, "여성본초학", pp.45~46 (2005), 전국의학사
- 김동일, "애엽 분말환을 이용한 질강내 훈증ㆍ훈연 및 온열 치료기(허브컴)의 한방부인과 임상응용에 관한 연구 1", 대한한방부인과학회지, 15(1), pp.77~88 (2002), 대한한방부인과학회
- 한방약리학교재편찬위원회, "한방약리학", pp.354~357 (2006), 도서출판 신일북스
- Umano K, "Volatile chemicals identified in extracts from leaves of Japanese mugwort(Artemisia princeps pamp,)", J Agric Food Chern, 48, pp.3463~3469 (2000)
- 조연희, "사자발쑥의 정유성분 및 항균 효과", 한국 국제농업개발학회지, 13(4), pp.313~320 (2001)
- 최호영, "강화산 艾葉의 보관기간에 따른 정유성분의 변화", 대한본초학회지, 22(4), pp.271~277 (2007), 대한본초학회
- Lee SH, "Arteminolides B, C, and D, new inhibitors of farnesyl protein transferase from Artemisia argyi", J Org Chern., 67, pp.7670~7675 (2002)
- Hayashi T, "Sulfated polysaccharide from the leaves of Artemisia Princeps activates heparin cofactor Ⅱ independently of the Lys173 and Arg189 residues of heparin cofactor Ⅱ", Thromb Res, 87, pp.105~112 (1997)
- Yoshikawa M, "Bioactive constituents of Chinese natural medicines. I. New sesquiterpene ketones with vasorelaxant effect from Chinese Moxa, the processed leaves of Artemisia argyi Levl. et Vant.: moxartenone and moxartenolide", Chern Pharm Bull(tokyo), 44, pp.1656~1662 (1996)
- 조경심, "필름 포장된 콩나물의 유통 중 축적되는 이취의 종류 및 발생 특성", 한국작물학회지, 51(3), pp.220~226 (2006), 한국작물학회
- 김호절, "한약약리학", pp.309~311 (2001), 집문당
- Jeong MA, "Jaceosidin, a pharmacologically active flavone drived from Artemisia argyi, inhibits phorbol -ester-induced upregulation of COX-2 and MMP-9 by blocking phosphorylation of ERK-1 and -2 in cultured human mammary epithelial cell", Ann NY Acad Sci, 1095, pp.458~466 (2007)
- Lee HG, "Inhibitory effect of jaceosidin isolated from Artemisia argyi on the function of E6 and E7 oncoproteins of HPV 16", J Ethnopharmacol, 98(3), pp.339~343 (2005)
- Lee SH, "Arteminolides B, C, and D, new inhibitors of farnesyl protein transferase from Artemisia argyi", J Org Chem, 67(22), pp.7670~7675 (2002)
- Adams M, "Activity -guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM", ADR5000 cells, from Artemisia argyi, 2006, pp.72(9), 862~864
- Wenqiang G, "Comparison of composition and antifungal activity of Artemisia argyi Lévl. et Vant inflorescence essential oil extracted by hydrodistillation and supercritical carbon dioxide", Nat Prod Res, 20(11), pp.992~998 (2006)
- 이영숙, "애엽(Artemisia argyi Levl, et Vant., Compositae, 황해쑥)의 항균력에 대한 연구", 생약학회지, 20(3), pp.249~ (1990)
- Kim MJ, "Jaceosidin induces apoptosis in ras-transformed human breast epithelial cells through generation of reactive oxygen species", Ann NY Acad Sci, 1095, pp.483~485 (2007)
- Zhong Y, "Effective chemical constituent of Artemisia argyi Levl. et Vant for inhibition of platelet aggregation", Zhongguo Zhong Yao Za Zhi, 17(6), pp.353~345 (1992)
- Yoshikawa M, "Bioactive constituents of Chinese natural medicines. I. New sesquiterpene ketones with vasorelaxant effect from Chinese moxa, the processed leaves of Artemisia argyi Levl. et Vant.: moxartenone and moxartenolide", Chem Pharm Bull, 44(9), pp.1656~1662 (1996)
- Nakasugi T, "Antimutagens in gaiyou(Artemisia argyi Levl. et Vant.)", J Agric Food Chem, 48(8), pp.3256~3266 (2000)
- Hwang YC, "Effect of acupuncture on young pigs with induced enteropathogenic Escherichia cili diarrhea", Am J Vet Res, 49(9), pp.1641~1643 (1988)
- Srivastava R, "Bioreactive polysaccharides from plants", Phytochem, 28, pp.2877~2883 (1989)
- Ignarro LJ, "Proc Natl Acad Sci USA", 84(24), pp.9265~9269 (1987)
- 國家中醫藥管理局中華本草編委會, "中華本草", pp.308~314 (1999), 上海科學技術出版社
- 김인훈, "음양곽 추출분획물이 마우스의 면역 반응에 미치는 영향", 석사, pp.1990
- 김창민, "中藥大辭典", pp.4400~4406 (1998), 도서출판 정담
- 김성렬, "음양곽추출물 중의 항산화성분 분리 밀 성질", Korean J Food Sci Technol, 24(6), pp.535~540 (1992)
- 이종원, "음양곽의 항산화활성", J Korean Soc Food Sci Nutr, 29(4), pp.732~736 (2000)
- 박승택, "배양 희소돌기아교세포에 있어서 산소자유기 神經毒性에 대한 淫羊藿의 效果", 대한동의병리학회지, 11(2), pp.58~62 (1997)
- 최형일, "淫羊藿의 항고혈압작용에 관한 연구", 大韓本草學會誌, 12(1), pp.35~44 (1997)
- 이영구, "음양곽의 추출물이 노화에 따른 흰쥐 간의 이물질대사 효소계에 미치는 영향", 한국약용작물학회지, 10(1), pp.6~36 (2002), 한국약용작물학회
- Kim JH, "Effects of aqueous extract of Epimedii Herba on the induction of oral tolerance in mice", Biol Pharm Bull, 25(8), pp.1000~1005 (2002)
- 김삼식, "음양곽의 Flavonoid 성분에 관한 연구", 생약학회지, 19(2), pp.93~96 (1988)
- Amellal M, "Inhibition of mast cell histamine release by flavonoids and biflavonoids planta Medica", 1, pp.16~ (1985)
- Elliot M, "Flavonoid inhibition of human basophil histamine release stimulated by various agents", Biochem Pharmacol, 33, pp.3333~ (1984)
- Tang W, "Chinese drugs of plant origin", pp.491~ (1992), Springer-Verlag
- 성낙계, "신판 발효공학", pp.14~15 (1995), 형설출판사
- 하덕모, "신편 발효공학", pp.288~ (1994), 문운당
- Lindemann RA, "Production of interleukin-1 and tumor necrosis factor by human peripheral blood monocytes activated by periodontal bacteria and extrocted lipopolysaccharide", J. Dent Res., 67(8), pp.1131~1135 (1988)
- Lehnardt S, "Activation of innate immunity in the CNS triggers neurodegeneration through a Toll-like receptor 4-dependent pathway", Proc Natl Acad Sci USA, 100(14), pp.8514~8519 (2003)
- Gelderman KA, "Macrophages suppress T cell responses and arthritis development in mice by producing reactive oxygen species", J Clin Invest, 117(10), pp.3020~3028 (2007)
- Hultqvist M, "Lack of reactive oxygen species breaks T cell tolerance to collagen type II and allows development of arthritis in mice", J Immunol, 179(3), pp.1431~1437 (2007)
- Hultqvist M, "Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene", Proc Natl Acad Sci USA, 101(34), pp.12646~12651 (2004)
- Garthwaite J, "Endothelium-derived relaxing factor release on activation of NMDA receptors suggests a role as intracellular messenger in the brain", Nature, 336, pp.385~388 (1988)
- Furchgott RF, "The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine", Nature, 288, pp.373~376 (1980)
- Albina JE, "Nitric oxide-mediated apoptosis in murine peritoneal macrophage", J Immunol, 150, pp.5080~5085 (1993)
- Fehsel K, "Nitric oxide induces apoptosis in mouse thymocytes", J Immunol, 155, pp.2858~2865 (1995)
- Michael R, "Essential function of nitric oxide synthase in Drosophila", Current Biology, 14, pp.881~882 (2004)
- McKnight GM, "Dietary nitrate in man: friend or foe", Br. J. Nutr, 81(5), pp.349~358 (1999)
- Busse R, "Induction of nitric oxide synthase by cytokines in vascular smooth muscle cells", FEBS Lett, 275, pp.87~90 (1990)
- 이혜성, "애엽으로 부터 분리한 수용성 추출물의 염증 억제 효과에 관한 연구", 석사, pp.2007
