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Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants

박완수
대한약침학회 (Korean Pharmacopuncture Institute)|2009.12|Journal of Pharmacopuncture|제12권 제4호|pp. 89-96

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참고문헌

키워드

fermentationmacrophagenitric oxideARTEMISIAE ARGI FOLIUMLactobacillus pentosus
주제분류한의학

초록

Objectives : The purpose of this study is to investigate the effect of Water Extract from Lactobacillus pentosus-fermented ARTEMISIAE ARGI FOLIUM (AFL) on nitric oxide production of mouse macrophage Raw 264.7 cells impaired by various toxicants such as gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. Methods : ARTEMISIAE ARGI FOLIUM was fermented with Lactobacillus pentosus and extracted by water. Nitric oxide production of mouse macrophage Raw 264.7 cells was measured by Griess reagent assay. Examined concentrations of AFL were 10, 50, 100, 200, 400 ug/mL. Results : The results of the experiment are as below. 1. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by gallic acid (100 uM) in Raw 264.7 cells. 2. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by EtOH (100 uM) in Raw 264.7 cells. 3. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by nicotine (1mM) in Raw 264.7 cells. 4. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaminophen(2 mM) in Raw 264.7 cells. 5. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaldehyde (200 uM) in Raw 264.7 cells. Conclusions : AFL could be supposed to have the immune-enhancing activity related with nitric oxide production of macrophage impaired by various toxicants.

Abstract

Objectives : The purpose of this study is to investigate the effect of Water Extract from Lactobacillus pentosus-fermented ARTEMISIAE ARGI FOLIUM (AFL) on nitric oxide production of mouse macrophage Raw 264.7 cells impaired by various toxicants such as gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. Methods : ARTEMISIAE ARGI FOLIUM was fermented with Lactobacillus pentosus and extracted by water. Nitric oxide production of mouse macrophage Raw 264.7 cells was measured by Griess reagent assay. Examined concentrations of AFL were 10, 50, 100, 200, 400 ug/mL. Results : The results of the experiment are as below. 1. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by gallic acid (100 uM) in Raw 264.7 cells. 2. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by EtOH (100 uM) in Raw 264.7 cells. 3. AFL at the concentration of 400 ug/mL significantly recovered nitric oxide production which was reduced by nicotine (1mM) in Raw 264.7 cells. 4. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaminophen(2 mM) in Raw 264.7 cells. 5. AFL at the concentration of 200, 400 ug/mL significantly recovered nitric oxide production which was reduced by acetaldehyde (200 uM) in Raw 264.7 cells. Conclusions : AFL could be supposed to have the immune-enhancing activity related with nitric oxide production of macrophage impaired by various toxicants.

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관련 논문AI추천

Gallic acid 등으로 유발된 대식세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향박완수한의병리학회 · 동의생리병리학회지2009 · 제23권 제2호EtOH 등으로 유발된 대식세포 내 hydrogen peroxide 생성억제에 대한 효모균발효애엽 추출물의 영향박완수한의병리학회 · 동의생리병리학회지2009 · 제23권 제3호고삼과 애엽의 발효 혼합물이 에탄올과 니코틴으로 유발된 마우스 대식세포 내 hydrogen peroxide 생성감소에 미치는 영향김도훈, 박완수한의병리학회 · 동의생리병리학회지2008 · 제22권 제5호EtOH 등의 독성물질에 대한 유산균발효애엽 추출물의 간세포보호효과박완수한의병리학회 · 동의생리병리학회지2010 · 제24권 제3호마우스 대식세포(Raw 264.7)에 대한 艾葉 물추출물의 생리활성 연구박완수한의병리학회 · 동의생리병리학회지2008 · 제22권 제4호애엽과 음양곽 혼합 발효물이 대식세포 활성에 미치는 영향김윤상, 류한우, 임은미대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제2호

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