본문 바로가기
KERD
논문KCI 등재

돼지 생식기호흡기증후군바이러스의 ORF6 유전자 발현

Expression of ORF6 gene of porcine reproductive and respiratory syndrome (PRRS) virus

김진원|강신영|윤영심|배수정
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2009.03|한국동물위생학회지|제32권 제1호|pp. 19-25

1

조회

0

다운로드

0

인용

0

참고문헌

주제분류수의학

초록

Abstract

Porcine reproductive and respiratory syndrome (PRRS) virus is the etiological agent of diseases characterized by reproductive losses in sows and respiratory disorders in piglets. The PRRS virus is a small enveloped virus containing a positive-sense, single-stranded RNA genome. In the present study, ORF6 gene of Korean PRRS virus isolate, CNV, was cloned and expressed in baculovirus expression system. The ORF6 gene and expressed protein in the recombinant virus were confirmed by PCR/indirect fluorescence antibody (IFA) test and Western blotting, respectively. The recombinant protein with a molecular weight of approximately 24KDa was confirmed by Western blotting using His6 and PRRS virus-specific antiserum. Expressed ORF6 protein was applied for IFA to detect antibody against PRRS virus using field porcine sera. However, the sensitivity and specificity of developed IFA using expressed ORF6 protein were considerably low compared to those of commercial ELISA kit. This results suggest that IFA using expressed ORF6 protein could not be used as a diagnostic test for PRRS virus infection without further improvements.

참고문헌 (20)

  1. 박최규, "돼지 생식기호흡기증후군 바이러스항체 검색에 있어 간접형광항체법(IFA)과 효소면역법(ELISA)의 진단효율 비교", 대한수의학회지, 38, pp.314~318 (1998)
  2. Albina E, "An enzyme linked immunosorbent assay (ELISA) for the detection of antibody to porcine reproductive and respiratory syndrome virus", Ann Rec Vet, 23, pp.167~176 (1992)
  3. Bautista EM, "Structural polypeptides of the American (VR-2332) strain of porcine reproductive and respiratory syndrome virus", Arch Virol, 141, pp.357~1365 (1998)
  4. Cavanagh D, "Nidovirales: A new order comprising Coronaviridae and Arteriviridae", Arch Virol, 142, pp.629~633 (1997)
  5. Dea S, "Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus", Arch Virol, 145, pp.659~688 (2000)
  6. Houben S, "Comparative study of a blocking enzyme linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibody to porcine reproductive and respiratory syndrome virus in pigs", J Virol Methods, 51, pp.125~128 (1995)
  7. Kamata K, "Expression and antigenicity of virus-like particles of norovirus and their application for detection of noroviruses in stool samples", J Med Virol, 6, pp.129~136 (2005)
  8. Keffaber KK, "Reproductive failure of unknown etiology", Am Assoc Swine Pract Newsletter, 1, pp.1~10 (1989)
  9. Kim HS, "Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line", Arch Virol, 133, pp.477~483 (1993)
  10. Kreutz LC, "Baculovirus expression and immunological detection of the major structural proteins of porcine reproductive and respiratory syndrome virus", Vet Microbiol, 59, pp.1~13 (1997)
  11. Perrin A, "Recombinant capripoxviruses expressing protein of bluetongue virus:evaluation of immune responses and protection in small ruminants", Vaccine, 25, pp.6774~6783 (2007)
  12. Qian P, "High-level experssion of the ORF6 gene of porcine reproductive and respiratory syndrome virus (PRRSV) in Pichia pastoris", Virus Genes, 27, pp.189~196 (2003)
  13. Snijder EJ, "The molecular biology of arteriviruses", J Gen Virol, 79, pp.961~979 (1998)
  14. Sorensen KJ, "Blocking ELISA’s for the distinction between antibodies against european and american strains of porcine reproductive and respiratory syndrome virus", Vet Microbiol, 60, pp.169~177 (1998)
  15. Stewart ME, "Baculovirus expression of beak and feather disease virus (BFDV) capsid protein capable of self-assembly and haemagglutination", J Virol Methods, 141, pp.181~187 (2007)
  16. Towbin H, "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications", Proc Natl Acad Sci USA, 76, pp.4350~4354 (1985)
  17. Wensvoort G, "Mystery swine disease in the Netherlands: The isolation of Lelystad virus", Vet Quart, 13, pp.121~130 (1991)
  18. Yoon IJ, "An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera", J Vet Diagn Invest, 4, pp.144~147 (1992)
  19. Yoon IJ, "A modified serum neutralization test for the detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera", J Vet Diahn Invest, 6, pp.289~292 (1994)
  20. Zimmerman JJ, "Porcine reproductive and respiratory syndrome virus (Porcine arterivirus). In: Disease of swine" (2006), Blackwell publishing

관련 논문AI추천

돼지생식기호흡기증후군바이러스 ORF7 유전자 발현 및 단크론항체 생산강신영, 류민상, 박가혜, 이경원, 이승철한국동물위생학회 · 한국동물위생학회지2014 · 제37권 제3호돼지 호흡기 코로나바이러스 감염증의 감별진단과 항체분포 조사김도경, 김은경, 손병국, 이종민한국동물위생학회 · 한국동물위생학회지2009 · 제32권 제4호ORF5a Protein of Porcine Reproductive and Respiratory Syndrome Virus is Indispensable for Virus Replication오종석, 이창희한국미생물·생명공학회 · 한국미생물·생명공학회지2015 · 제43권 제1호안동과 합천 지역 양돈장의 돼지생식기호흡기증후군(PRRS) 조사강혜원, 송재영, 오윤이, 최은진한국동물위생학회 · 한국동물위생학회지2014 · 제37권 제1호돼지 써코바이러스 2형 국내분리주의 유전학적 특성 규명김문, 한정희한국동물위생학회 · 한국동물위생학회지2009 · 제32권 제1호돼지생식기호흡기증후군 바이러스가 흉막폐렴에 미치는 영향(혈청학적 및 백혈구아군 분포율에 관한 연구)박용호, 정현규, 한정희한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제1호

이 간행물의 다른 논문 (11건)

돼지 써코바이러스 2형 국내분리주의 유전학적 특성 규명한정희, 김문pp. 1-10한국 이유자돈에서 검출된 돼지 유행성 설사 바이러스의 유전자 서열 분석김영훈, 신현근, 한정희, 서태원pp. 11-18Seroprevalence of selected porcine respiratory pathogens in the pig herds in Chungcheong and Gyeongsang provinces in Korea박창식, Xu-Min Hu, 전무형, Jia-Qi Chu, 김명철pp. 27-32양돈용 생균제 균주개발을 위한 유산균주 선발한선경, 신명수, 이완규, 유지숙pp. 33-41닭에서 분리한 Salmonella Gallinarum의 생화학적 특성, 약제감수성과 polymerase chain reaction을 이용한 신속진단송희종, 이정원, 추금숙pp. 43-48
원문 보기