안동과 합천 지역 양돈장의 돼지생식기호흡기증후군(PRRS) 조사
Survey of porcine reproductive and respiratory syndrome (PRRS) on pig farms in Andong and Hapcheon region
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초록
Porcine reproductive and respiratory syndrome (PRRS) causes a significant economic loss in the swine industry not only in Korea but also all over the world. Andong and Hapcheon region were selected for Area Regional Control (ARC) programme to reduce the shedding of PRRS virus (PRRSV) and de-crease PRRS outbreaks. Before conducting the PRRS ARC, sera of pigs were tested for both antibody using ELISA and antigen using RT-PCR, then phylogenetic classifications was analysed. Pigs of 138/275 (50.2%) in Andong and 352/425 (82.8%) in Hapcheon were seropositive. Also, the RT-PCR results revealed that 27 heads (8.2%) in Andong, 112 heads (22.0%) in Hapcheon were positive for PRRSV antigen. PRRSVs were mainly detected between the ages of 40 to 60 days. PRRSV ORF5 re-gions were used to determine genetic clusters based on previous report. All PRRSV type I detected in both Andong and Hapcheon were classified as Cluster I. The PRRSV type II isolates in Andong were assorted to Cluster II, whereas the PRRSV type II isolates in Hapcheon were the viruses were un-assembled into any cluster except one identified to Cluster III. Phylogenetic analysis indicated that new clusters of PRRSVs type II were prevalent in Hapcheon.
Abstract
Porcine reproductive and respiratory syndrome (PRRS) causes a significant economic loss in the swine industry not only in Korea but also all over the world. Andong and Hapcheon region were selected for Area Regional Control (ARC) programme to reduce the shedding of PRRS virus (PRRSV) and de-crease PRRS outbreaks. Before conducting the PRRS ARC, sera of pigs were tested for both antibody using ELISA and antigen using RT-PCR, then phylogenetic classifications was analysed. Pigs of 138/275 (50.2%) in Andong and 352/425 (82.8%) in Hapcheon were seropositive. Also, the RT-PCR results revealed that 27 heads (8.2%) in Andong, 112 heads (22.0%) in Hapcheon were positive for PRRSV antigen. PRRSVs were mainly detected between the ages of 40 to 60 days. PRRSV ORF5 re-gions were used to determine genetic clusters based on previous report. All PRRSV type I detected in both Andong and Hapcheon were classified as Cluster I. The PRRSV type II isolates in Andong were assorted to Cluster II, whereas the PRRSV type II isolates in Hapcheon were the viruses were un-assembled into any cluster except one identified to Cluster III. Phylogenetic analysis indicated that new clusters of PRRSVs type II were prevalent in Hapcheon.
참고문헌 (18)
- Albina E, "Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview", Vet Microbiol, 55, pp.309~316 (1997)
- Ansari IH, "Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies", J Virol, 80, pp.3994~4004 (2006)
- Baron T, "Report on the first outbreaks of the porcine reproductive and respiratory syndrome (PRRS) in France. Diagnosis and viral isolation", Ann Rech Vet, 23, pp.161~166 (1992)
- Cavanagh, D, "Nidovirales: a new order comprising Coronaviridae and Arteriviridae", Arch Virol, 142, pp.629~633 (1997)
- Cho JG, "Porcine reproductive and respiratory syndrome virus", Theriogenology, 66, pp.655~662 (2006)
- 최은진, "Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea", Journal of Veterinary Science, 14(2), pp.115~124 (2013), 대한수의학회[DOI]
- 최은진, "Survey on porcine reproductive and respiratory disease virus infection in pig farms associated with wasting and respiratory syndrome during 2005~2009 in Korea", 예방수의학회지, 35(1), pp.29~33 (2011), 한국예방수의학회
- Christianson WT, "Porcine reproductive and respiratory syndrome: a review", Swine Health Prod, 2, pp.10~28 (1994)
- Collins JE, "Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs", J Vet Diagn Invest, 4, pp.117~126 (1992)
- Key KF, "Genetic variation and phylogenetic analyses of the ORF5 gene of acute porcine reproductive and respiratory syndrome virus isolates", Vet Microbiol, 83, pp.249~263 (2001)
- Young S. Lyoo, "Serological and genetic characterization of the Europeanstrain of the porcine reproductive and respiratorysyndrome virus isolated in Korea", 대한수의학회지, 46(4), pp.363~370 (2006), 대한수의학회
- Kweon CH, "Isolation of porcine reproductive and respiratory syndrome virus (PRRSV) in Korea.", Korean J Vet Res, 34, pp.77~83 (1994)
- Neumann EJ, "Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States", J Am Vet Med Assoc, 227, pp.385~392 (2005)
- Pesente P, "Phylogenetic analysis of ORF5 and ORF7 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) from PRRS-positive Italian farms: showcase for PRRSV epidemiology and its consequences on farm management", Vet Microbiol, 114, pp.214~224 (2006)
- Rossow KD, "Pathogenesis of porcine reproductive and respiratory syndrome virus infection in gnotobiotic pigs", Vet Pathol, 32, pp.361~373 (1995)
- Wayne SR, "Potential role of noncommercial swine populations in the epidemiology and control of porcine reproductive and respiratory syndrome virus", J Am Vet Med Assoc, 240, pp.876~882 (2012)
- Wills RW, "Porcine reproductive and respiratory syndrome virus: routes of excretion", Vet Microbiol, 57, pp.69~81 (1997)
- Zimmerman JJ, "General overview of PRRSV: a perspective from the United States", Vet Microbiol, 55, pp.187~196 (1997)