Genistein이 섬유주세포의 생존과 일산화질소의 생성에 미치는 영향
Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells
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목적: Genistein이 섬유주세포의 생존과 일산화질소의 생성에 미치는 영향에 대해서 알아보고자 하였다. 대상과 방법: 섬유주세포를 일차배양한 후 혈청이 결핍된 배지를 이용하여 0, 1, 10 μm의 genistein으로 처리한 후 10분, 1일에 Griess assay와RT-PCR을 이용하여 일산화질소의 생성과 eNOS의 활성을 각각 측정하였으며, 1일째에 MTT assay를 이용하여 세포의 생존을 조사하였다. 결과: Genistein은 노출 후 10분 후에 10 μm에서, 1일째에 1 μm에서 각각 일산화질소의 생성증가시켰다. 또한 Genistein은 eNOS의활성을 증가시켰으며, 1일째에 세포의 생존을 유의하게 증가시켰다. 결론: Genistein은 혈청 결핍상태에서 단기간과 장기간에 일산화질소의 생성을 촉진시키면서 섬유주세포의 생존을 증가시키는 효과를나타내었다.
Abstract
Purpose: To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0, 1, and 10 μm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day. Results: Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 μm and for 1 day at 1 μm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC. Conclusions: Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures.
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