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섬유주세포에서 Erythropoietin이 일산화질소의 생성에 미치는 영향

Effect of Erythropoietin on the Production of Nitric Oxide in Trabecular Meshwork Cells

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목적: Erythropoietin (EPO)이 배양된 섬유주세포에서 일산화질소의 생성에 미치는 영향을 알아보고자 하였다. 대상과 방법: 섬유주세포를 일차배양한 후 혈청이 결핍된 배지를 이용하여 0, 0.5, 1.0 U/ml의 EPO에 이틀간 노출시킨 후 Griess assay를 이용하여 일산화질소의 생성량을 측정하였고, RT-PCR을 이용하여 EPO 수용체 mRNA와 eNOS의 발현을 조사하였다. 결과: EPO은 혈청결핍상태에서 섬유주세포의 생존에 영향을 미치지 않았다(p>0.05). 섬유주세포에서 EPO 수용체 mRNA가 발현되었다. EPO는 일산화질소의 생성을 증가시켰으며(p<0.05) eNOS의 발현도 증가되었다. 결론: EPO는 섬유주세포의 생존에 영향을 주지 않았다. EPO은 eNOS의 발현을 증가시켰으며 일산화질소의 생성을 증가시켰다.

Abstract

Purpose: To investigate the effects of erythropoietin (EPO) on the production of nitric oxide in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0, 0.5, and 1.0 U/ml EPO using serum-deprived media for 2 days. Production of nitric oxide and cellular survival were assessed with Griess assay and MTT assay, respectively. Expression of EPO mRNA receptor and activity of eNOS were assessed with RT-PCR. Results: EPO did not affect the survival of HTMC (p > 0.05). EPO increased the production of nitric oxide (p < 0.05) accompanied with increased eNOS activity. Conclusions: EPO had no effect on the cellular survival under serum-deprived conditions. EPO increases the production of nitric oxide by increasing eNOS activity.

참고문헌 (29)

  1. Alvarado J, "Trabecular meshwork cellularity in primary open-angle glaucoma and nonglaucomatous normals", Ophthalmology, 91, pp.564~579 (1984)
  2. Rohen JW, "Ultrastructure of the trabecular meshwork in untreated cases of primary open-angle glaucoma", Exp Eye Res, 56, pp.683~692 (1993)
  3. Wiederholt M, "Effect of diuretics, channel modulators, and signal interceptors on contractility of the trabecular meshwork", Ophthalmologica, 211, pp.153~160 (1997)
  4. Wiederholt M, "The trabecular meshwork and aqueous humor reabsorption", pp.163~202 (1998), Academic
  5. Wiederholt M, "Lepple-Wienhues A. Relaxation of trabecular meshwork and ciliary muscle by release of nitric oxide", Invest Ophthalmol Vis Sci, 35, pp.2515~2520 (1994)
  6. Behar-Cohen FF, "Decreased intraocular pressure induced by nitric oxide donors is correlated to nitrite production in the rabbit eye", Invest Ophthalmol Vis Sci, 37, pp.1711~1715 (1996)
  7. Fandrey J, "Oxygen-dependent and tissue-specific regulation of erythropoietin gene expression", Am J Physiol Regul Integr Comp Physiol, 286, pp.977~988 (2004)
  8. Siren AL, "Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress", Proc Natl Acad Sci USA, 98, pp.4044~4049 (2001)
  9. Carlini RG, "Effect of recombinant human erythropoietin on endothelial cell apoptosis", Kidney Int, 55, pp.546~553 (1999)
  10. Akimoto T, "Erythropoietin regulates vascular smooth muscle cell apoptosis by a phosphatidylinositol 3 kinase-dependent pathway", Kidney Int, 58, pp.269~282 (2000)
  11. Rui T, "Erythropoietin prevents the acute myocardial inflammatory response induced by ischemia", reperfusion via induction of AP-1, 2005, pp.65, 719~727
  12. Burger D, "Geoghegan-Morphet N, et al. Erythropoietin protects cardiomyocytes from apoptosis via up-regulation of endothelial nitric oxide synthase", Cardiovasc Res, 72, pp.51~59 (2006)
  13. Garcia-Ramirez M, "Expression of Erythropoietin and Its Receptor in the Human Retina: comparative study of diabetic and nondiabetic subjects", Diabetes Care, 31, pp.1189~1194 (2008)
  14. sai JC, "Erythropoietin: A candidate neuroprotective agent in the treatment of glaucoma", J Glaucoma, 16, pp.567~571 (2007)
  15. Mosmann T, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
  16. assay is a fast and reliable method for colorimetric determination of fungal cell densities, "wAppl Environ Microbiol", 1999, 3727~3729
  17. Nitrate in biological fluids, "Anal Biochem", 1982, 131~138
  18. Smith KJ, "The cardiovascular effects of erythropoietin", Cardiovasc Res, 59, pp.538~548 (2003)
  19. Santhanam AV, "Role of endothelial NO synthase phosphorylation in cerebrovascular protective effect of recombinant erythropoietin during subarachnoid hemorrhage- induced cerebral vasospasm", Stroke, 36, pp.2731~2737 (2005)
  20. Urao N, "Erythropoietin-mobilized endothelial progenitors enhance reendothelialization via Akt-endothelial nitric oxide synthase activation and prevent neointimal hyperplasia", Circ Res, 98, pp.1405~1413 (2006)
  21. d’Uscio LV, "Essential role of endothelial nitric oxide synthase in vascular effects of erythropoietin", Hypertension, 49, pp.1142~1148 (2007)
  22. d’Uscio LV, "Erythropoietin increases endothelial biosynthesis of tetrahydrobiopterin by activation of protein kinase B alpha", Akt1, 2008, pp.52, 93~99
  23. d’Uscio LV, "Erythropoietin increases expression and function of vascular copper- and zinc-containing superoxide dismutase", Hypertension, 55, pp.998~1004 (2010)
  24. Wang XQ, "Erythropoietin depresses nitric oxide synthase expression by human endothelial cells", Hypertension, 33, pp.894~899 (1999)
  25. Mokbel TH, "Erythropoietin and soluble CD44 levels in patients with primary open-angle glaucoma", Clin Experiment Ophthalmol, 38, pp.560~565 (2010)
  26. Kawakami M, "Erythropoietin receptor- mediated inhibition of exocytotic glutamate release confers neuroprotection during chemical ischemia", J Biol Chem, 276, pp.39469~39475 (2001)
  27. Arjamaa O, "Oxygen-dependent diseases in the retina: role of hypoxia-inducible factors", Exp Eye Res, 83, pp.473~483 (2006)
  28. Milano M, "Erythropoietin and neuroprotection: a therapeutic perspective", J Oncol Pharm Pract, 11, pp.145~149 (2005)
  29. Zhong Y, "Promotion of neurite outgrowth and protective effect of erythropoietin on the retinal neurons of rats", Graefes Arch Clin Exp Ophthalmol, 245, pp.1859~1867 (2007)

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