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일산화질소가 섬유주세포의 유착과 이동에 미치는 영향

Effect of Nitric Oxide on Adhesion and Migration of Trabecular Meshwork Cells

김재우|배진성
대한안과학회 (The Korean Ophthalmological Society)|2013.04|대한안과학회지 (Journal of The Korean Ophthalmological Society)|제54권 제4호|pp. 639-644

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참고문헌

키워드

MigrationAdhesionNitric oxideTrabecular meshwork cells
주제분류안과학

초록

목적: 일산화질소(nitric oxide, NO)가 섬유주세포의 유착과 이동성에 미치는 영향을 알아보고자 하였다. 대상과 방법: 사람의 섬유주세포를 일차배양한 다음, 세포의 유착성을 알아보기 위하여 한 시간 동안 배양용기에 부착한 다음 세척후에 남아 있는 세포를 MTT assay로 조사하였다. 세포의 이동성의 정도는 microchemoattraction chamber를 이용하여 정상적인 조건과 기계적 스트레스를 가한 조건에서 각각 조사하였다. 이때 NO 제공자인 S-nitroso-N-acetylpenicillamine (SNAP)에 노출시켜 NO 가 섬유주세포의 유착성과 이동성에 미치는 영향을 알아보았다. 결과: NO는 섬유주세포의 유착성과 이동성의 정도에는 영향을 미치지 않았다(p＞0.05). 1% 혈청을 첨가한 경우 유착이 증가하였으며이동성이 감소하였다(p＜0.05). 기계적 스트레스를 가한 경우 이동성의 정도가 감소하였다(p＜0.05). 결론: NO는 배양된 사람의 섬유주세포에서 세포의 유착성과 이동성에는 영향을 미치지 않을 것으로 생각한다.

Abstract

Purpose: To investigate the effect of nitric oxide (NO) on the adhesion and migration of cultured human trabecular meshwork cells (HTMC). Methods: For adhesion assay, primarily cultured HTMC were attached to culture dishes for 1 hr, cells were rinsed, and the remaining adherent cells were assessed with MTT assay. Degree of cellular migration was assessed under normal and stressed conditions using microchemoattraction chambers. Effect of NO on the adhesion and migration was assessed with or without co-exposure of S-nitroso-N-acetylpenicillamine (SNAP). Results: NO did not affect the degree of adhesion or migration of HTMC (p ＞ 0.05). The degree of adhesion increased although the degree of migration decreased with 1% serum (p < 0.05). Degrees of migrations decreased after mechanical stress (p < 0.05). Conclusions: NO may not affect the adhesion or migration of HTMC.

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