쥐의 외안근 부분 절개술 후 데코린과 종양성장인자-베타의 발현
Decorin and TGF-β Expression after Partial Myotomy of the Extraocular Muscle in Rat
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
목적: 쥐의 외안근에 부분 절개를 시행한 후, decorin과 TGF-β의 발현을 확인해보고자 하였다. 대상과 방법: 수컷 Sprague-Dawley (SD)계 흰쥐 열 마리의 우안 상직근을 노출시킨 후 부분 절개술을 시행하였고, 좌안은 상직근을노출시킨 후 결막을 다시 봉합하였다. 수술 2주일 후 양안에서 상직근을 채취하여 Hematoxylin-eosin, Masson’s trichrome 염색 및면역조직화학염색을 시행하였다. 결과: 대조군에 비해 실험군에서 Hematoxylin-Eosin 및 Masson’s trichrome으로 염색한 조직 주변으로 불규칙하고 밀집되어 있는섬유 다발이 많이 관찰되었으며, 면역조직화학법에서 손상 받은 근육 세포주변에서 decorin과 TGF-β가 강하게 발현한 것을 확인할수 있었으며, 이중염색에서 TGF-β가 발현한 자리에서 decorin이 함께 발현한 모습을 확인할 수 있었다. 결론: 쥐의 외안근을 부분 절개한 실험군에서 decorin이 발현하는 것을 확인할 수 있었으며, 이중 면역조직화학염색을 통해 TGF-β가발현하는 위치에서 decorin이 함께 발현하는 것을 확인하였다.
Abstract
Purpose: To report the expression of decorin and TGF-β in partial myotomy of the extraocular muscle in rats. Methods: Partial myotomy of the superior rectus muscle was performed on the right eye of 10 Sprague-Dawley rats followed by exposure of the left superior rectus muscle and a simple suture of the conjunctiva. The bilateral superior rectus muscle was obtained from all rats at 2 weeks postoperatively. The tissues were observed under light microscopy with hematoxylin-eosin, Masson’s trichrome staining and immunohistochemistry. Results: Histological examinations of the surgical area at 2 weeks after postoperatively showed irregularly concentrated fibrosis on light microscopy with hematoxylin-eosin and Masson’s trichrome staining of the experimental eyes. Immnohistochemistry showed that expression of decorin was in the same location as TGF-β in the experimental group. Conclusions: The expression of decorin was found in the healing process after partial myotomy of the extraocular muscle in rats. Immunohistochemistry showed that expression of decorin was in the same location as with TGF-β.
참고문헌 (31)
- Metz HS., "Restrictive factors in strabismus", Surv Ophthalmol, 28, pp.71~83 (1983)
- Dunlap, EA., "Surgery of muscle adhesions and effects of multiple operations", Br J Ophthalmol, 58, pp.307~312 (1974)
- Gomel V, "Pathophysiology of adhesion formation and strategies for prevention", J Reprod Med, 41, pp.35~41 (1996)
- Hwang JM, "Use of Viscoat for delayed postoperative adjustable suture strabismus surgery in rabbits", Binocul Vis Strabismus Q, 11, pp.137~142 (1996)
- Hwang JM, "Delayed reattachment of extraocular muscles in rabbits using thin polytetrafluoroethylene", Ophthalmic Surg Lasers, 28, pp.59~64 (1997)
- Oh SO, "Effect of mitomycin C on delayed adjustment in experimental strabismus surgery", Korean J Ophthalmol, 9, pp.51~58 (1995)
- Kim JH, "Use of polyurethane with sustained release dexamethasone in delayed adjustable strabismus surgery", Br J Ophthalmol, 88, pp.1450~1454 (2004)
- Hwang JM, "Combined effect of Interceed and 5-fluorouracil on delayed adjustable strabismus surgery", Br J Ophthalmol, 83, pp.788~791 (1999)
- Siriwardena D, "Human antitransforming growth factor beta(2) monoclonal antibody--a new modulator of wound healing in trabeculectomy:a randomized placebo controlled clinical study", Ophthalmolgy, 109, pp.427~431 (2002)
- Border WA, "Natural inhibitor of transforming growth factor-beta protects against scarring in experimental kidney disease", Nature, 360(6402), pp.361~364 (1992)
- He F, "Upregulation of decorin by FXR in vascular smooth muscle cells", Biochem Biophys Res Commun, 372, pp.746~751 (2008)
- Zanotti S, "Decorin and biglycan expression is defferentially altered in several muscular dystrophies", Brain, 128(Pt 11), pp.2546~2555 (2005)
- Hocking AM, "Leucine-rich repeat glycoproteins of the extracellular matrix", Matrix Biol, 17, pp.1~19 (1998)
- Thieszen SL, "Expression of collagens and decorin during aortic arch artery development: implications for matrix pattern formation", Matrix Biol, 14, pp.573~582 (1995)
- Merle B, "Decorin inhibits cell attachment to thrombospondin-1 by binding to a KKTR-dependent cell adhesive site present within the N-terminal domain of thrombospondin- 1", J Cell Biochem, 67, pp.75~83 (1997)
- Merle B, "Decorin inhibits cell migration through a process requiring its glycosaminoglycan side chain", J Cell Biochem, 75, pp.538~546 (1999)
- De Luca A, "Decorin-induced growth suppression is associated with up-regulation of p21, an inhibitor of cyclin- dependent kinases", J Biol Chem, 271, pp.18961~18965 (1996)
- Fischer JW, "Retroviral overexpression of decorin differentially affects the response of arterial smooth muscle cells to growth factors", Arterioscler Thromb Vasc Biol, 21, pp.777~784 (2001)
- Hildebrand A, "Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor beta", Biochem J, 302(Pt 2), pp.527~534 (1994)
- Kaname S, "Betaglycan has multiple binding sites for transforming growth factor-beta 1", Biochem J, 315(Pt 3), pp.815~820 (1996)
- Honardoust D, "Small leucine-rich proteoglycans, decorin and fibromodulin, are reduced in postburn hypertrophic scar", Wound Repair Regen, 19, pp.368~378 (2011)
- Reich-Schupke S, "Decorin expression with stump recurrence and neovascularization after varicose vein surgery--a pilot study", Dermatol Surg, 37, pp.480~485 (2011)
- Grefte S, "Model for muscle regeneration around fibrotic lesions in recurrent strain injuries", Med Sci Sports Exerc, 42, pp.813~819 (2010)
- Aärimaa V, "Restroration of myofiber continuity after transection injury in the rat soleus", Neuromuscul Disord, 14, pp.421~428 (2004)
- 우경진, "토끼에서 근절제술 후 베바시주맙 결막하주사 효과", 대한안과학회지, 51(3), pp.423~429 (2010), 대한안과학회
- Border WA, "Natural inhibitor of transforming growth factor-beta protects against scarring in experimental kidney disease", Nature, 360, pp.361~364 (1992)
- Mohan RR, "Decorin biology, expression, function and therapy in the cornea", Curr Mol Med, 11, pp.110~128 (2011)
- Isaka Y, "Gene therapy by skeletal muscle expression of decorin prevents fibrotic disease in rat kidney", Nat Med, 2, pp.418~423 (1996)
- Giri SN, "Antifibrotic effect of decorin in a bleomycin hamster model of lung fibrosis", Biochem Pharmacol, 54, pp.1205~1216 (1997)
- Fukushima K, "The use of an antifibrosis agent to improve muscle recovery after laceration", Am J Sports Med, 29, pp.394~402 (2001)
- Grisanti S, "Decorin modulates wound healing in experimental glaucoma filtration surgery: a pilot study", Invest Ophthalmol Vis Sci, 46, pp.191~196 (2005)