상품화된 성장인자 제품이 다공성 안와충전물의 섬유혈관화에 미치는 영향
The Effects of Commodified Growth Factor Products on the Fibrovascularization of Porous Polyethylene Orbital Implants
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목적: 임상적으로 사용되고 있는 상품화된 성장인자 제품이 다공성 안와충전물 내로의 섬유혈관증식에 미치는 효과에 대해 알아보고자 하였다. 대상과 방법: 흰 쥐 18마리를 대상으로 Nepidermin (Easyef®), Trafermin (Fiblast®)과 대조군으로 생리식염수로 전처치한 다공성 폴리에틸렌 안와충전물(porous polyethylene orbital implant, Medpor®)을 등쪽 피하조직에 삽입하였다. 술 후 각 1주, 2주째 Medpor®를 적출하여 섬유혈관화 정도를 비교하였다. 섬유혈관화 정도는 혈관화가 된 길이를 백분율로 계산하여 통계 처리하였다. 결과: 술 후 1주째 적출한 Medpor®의 섬유혈관화 정도는 Easyef® 처치군 25.33 ± 5.43%, Fiblast® 처치군 22.56 ± 5.30%, 대조군21.78 ± 4.66%로 Easyef® 처치군이 다른 두 군과 비교하여 의미 있는 차이를 보였다(p=0.020, 0.012). 술 후 2주째 적출한 Medpor®의 섬유혈관화 정도는 Easyef® 처치군 98.33 ± 5.00%, Fiblast® 처치군 100.00 ± 0.00%로 대조군 95.89 ± 4.57%와 비교하여섬유혈관화가 증가되어 섬유혈관화가 촉진된 것을 알 수 있었다(p=0.019, <0.001). 결론: 타과 영역에서 상품화되어 사용 중인 성장인자 제품이 성형안과 수술에서도 선택된 경우에 섬유혈관증식을 촉진시켜 도움이될 수 있음을 알 수 있었다.
Abstract
Purpose: To investigate the effects of commodified growth factor products used clinically on fibrovascular ingrowth into porouspolyethylene orbital implants. Methods: Porous polyethylene orbital implant sheets (Medpor®) soaked with Nepidermin (Easyef®), Trafermin (Fiblast®), and normalsaline were implanted into the backs of 18 Sprague-Dawley rats. The degree of fibrovascular ingrowth as observed using a light microscopewas compared 1 and 2 weeks after implantation and was calculated as a percentage of the fibrovascular ingrowth length. Results: One week after implantation, the percentage of fibrovascular ingrowth length was 25.33 ± 5.43%, 22.56 ± 5.30%, and21.78 ± 4.66% in the Easyef®-, Fiblast®- and normal saline-soaked groups. The degree of fibrovascularization was higher in theEasyef®-soaked group than in the other groups (p = 0.020, 0.012). Two weeks after implantation, the degree of fibrovascularizationwas 98.33 ± 5.00%, 100.00 ± 0.00%, and 95.89 ± 4.57%, which was significantly higher in the Easyef®-, and Fiblast®-soakedgroups than in normal saline-soaked group (p = 0.019, <0.001). Conclusions: Commodified growth factor products used in other areas selectively enhanced fibrovascular ingrowth to a greaterdegree and earlier in ophthalmic plastic and reconstructive surgery.
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