Extracellular Vesicles Derived from Hypoxic Human Mesenchymal Stem Cells Attenuate GSK3β Expression via miRNA-26a in an Ischemia-Reperfusion Injury Model
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Purpose: Bioactive molecules critical to intracellular signaling are contained in extracellular vesicles (EVs) and have cardioprotectiveeffects in ischemia/reperfusion (IR) injured hearts. This study investigated the mechanism of the cardioprotective effectsof EVs derived from hypoxia-preconditioned human mesenchymal stem cells (MSCs). Materials and Methods: EV solutions (0.4 μg/μL) derived from normoxia-preconditioned MSCs (EVNM) and hypoxia-preconditionedMSCs (EVHM) were delivered in a rat IR injury model. Successful EV delivery was confirmed by the detection of PKH26staining in hearts from EV-treated rats. Results: EVHM significantly reduced infarct size (24±2% vs. 8±1%, p<0.001), and diminished arrhythmias by recovering electricalconduction, INa current, and Cx43 expression. EVHM also reversed reductions in Wnt1 and β-catenin levels and increases in GSK3βinduced after IR injury. miRNA-26a was significantly increased in EVHM, compared with EVNM, in real-time PCR. Finally, in in vitroexperiments, hypoxia-induced increases in GSK3β expression were significantly reduced by the overexpression of miRNA-26a. Conclusion: EVHM reduced IR injury by suppressing GSK3β expression via miRNA-26a and increased Cx43 expression. Thesefindings suggest that the beneficial effect of EVHM is related with Wnt signaling pathway.
Abstract
Purpose: Bioactive molecules critical to intracellular signaling are contained in extracellular vesicles (EVs) and have cardioprotectiveeffects in ischemia/reperfusion (IR) injured hearts. This study investigated the mechanism of the cardioprotective effectsof EVs derived from hypoxia-preconditioned human mesenchymal stem cells (MSCs). Materials and Methods: EV solutions (0.4 μg/μL) derived from normoxia-preconditioned MSCs (EVNM) and hypoxia-preconditionedMSCs (EVHM) were delivered in a rat IR injury model. Successful EV delivery was confirmed by the detection of PKH26staining in hearts from EV-treated rats. Results: EVHM significantly reduced infarct size (24±2% vs. 8±1%, p<0.001), and diminished arrhythmias by recovering electricalconduction, INa current, and Cx43 expression. EVHM also reversed reductions in Wnt1 and β-catenin levels and increases in GSK3βinduced after IR injury. miRNA-26a was significantly increased in EVHM, compared with EVNM, in real-time PCR. Finally, in in vitroexperiments, hypoxia-induced increases in GSK3β expression were significantly reduced by the overexpression of miRNA-26a. Conclusion: EVHM reduced IR injury by suppressing GSK3β expression via miRNA-26a and increased Cx43 expression. Thesefindings suggest that the beneficial effect of EVHM is related with Wnt signaling pathway.
참고문헌 (40)
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