Antioxidant and Anti-Inflammatory Effect of Probiotic Lactobacillus plantarum KU15149 Derived from Korean Homemade Diced-Radish Kimchi
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초록
Lactobacillus plantarum KU15149 was demonstrated to have probiotic behavior and functions, including antioxidant and anti-inflammatory activity. L. plantarum KU15149 obtained from homemade diced-radish kimchi has a high survival rate under artificial gastric acid (pH 2.5, 0.3% pepsin) and bile salt (0.3% oxgall) conditions. However, L. plantarum KU15149 did not produce β-glucuronidase, which is known to be a carcinogenic enzyme with resistance to several antibiotics, such as gentamycin, kanamycin, streptomycin, tetracycline, and ciprofloxacin. L. plantarum KU15149 strongly adhered to HT-29 cells and had high antioxidant activity in terms of 2,2-diphenyl- 1-picrylhydrazyl (DPPH) free radical-scavenging and β-carotene bleaching assays. L. plantarum KU15149 also exhibited a pronounced inhibition of nitric oxide (NO) production, along with expression of nitric oxide synthase (iNOS) and cyclooxygenase -2 (COX-2) as well as proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6, when RAW 264.7 cells were stimulated with LPS. Therefore, L. plantarum KU15149 exhibited pharmaceutical functionality as a potential probiotic.
Abstract
Lactobacillus plantarum KU15149 was demonstrated to have probiotic behavior and functions, including antioxidant and anti-inflammatory activity. L. plantarum KU15149 obtained from homemade diced-radish kimchi has a high survival rate under artificial gastric acid (pH 2.5, 0.3% pepsin) and bile salt (0.3% oxgall) conditions. However, L. plantarum KU15149 did not produce β-glucuronidase, which is known to be a carcinogenic enzyme with resistance to several antibiotics, such as gentamycin, kanamycin, streptomycin, tetracycline, and ciprofloxacin. L. plantarum KU15149 strongly adhered to HT-29 cells and had high antioxidant activity in terms of 2,2-diphenyl- 1-picrylhydrazyl (DPPH) free radical-scavenging and β-carotene bleaching assays. L. plantarum KU15149 also exhibited a pronounced inhibition of nitric oxide (NO) production, along with expression of nitric oxide synthase (iNOS) and cyclooxygenase -2 (COX-2) as well as proinflammatory cytokines, such as TNF-α, IL-1β, and IL-6, when RAW 264.7 cells were stimulated with LPS. Therefore, L. plantarum KU15149 exhibited pharmaceutical functionality as a potential probiotic.
참고문헌 (36)
- Rahman T, "Oxidative stress and human health", Adv. Biosci. Biotechnol, 3, pp.997~1019 (2012)
- Carocho M, "A review on antioxidants, prooxidants and related controversy : natural and synthetic compounds, screening and analysis methodologies and future perspectives", Food Chem. Toxicol, 51, pp.15~25 (2013)
- Dröge W, "Free radicals in the physiological control of cell function", Physiol. Rev, 82, pp.47~95 (2002)
- Lawrence T, "Anti-inflammatory lipid mediators and insights into the resolution of inflammation", Nat. Rev. Immunol, 2, pp.787~795 (2002)
- Duque GA, "Macrophage Cytokines : Involvement in immunity and infectious diseases", Front Immunol, 5, pp.491~ (2014)
- Working group report on drafting guidelines for the evaluation of probiotics in food, "FAO", World Health Organization and Food and Agriculture Organization of the United Nations, ~ (2002)
- Khan I, "Probiotic potential of nutritionally improved Lactobacillus plantarum DGK-17 isolated from kimchi-A traditional Korean fermented food", Food Control, 60, pp.88~94 (2016)
- Tokatlı M, "In vitro properties of potential probiotic indigenous lactic acid bacteria originating from traditional pickles", Biomed. Res. Int, 2015, pp.315819~ (2015)
- Shao Y, "Comparative studies on antibiotic resistance in Lactobacillus casei and Lactobacillus plantarum", Food Control, 50, pp.250~258 (2015)
- Lee NK, "Probiotic potential of Lactobacillus strains with anti-allergic effects from kimchi for yogurt starters", LWT-Food Sci. Technol, 58, pp.130~134 (2014)
- Son SH, "Potential probiotic Lactobacillus plantarum Ln4 from kimchi : Evaluation of β-galactosidase and antioxidant activities", LWT-Food Sci. Technol, 85, pp.181~186 (2017)
- Cuello-Garcia CA, "Probiotics for the prevention of allergy : A systematic review and meta-analysis of randomized controlled trials", J. Allergy Clin. Immunol, 136, pp.952~961 (2015)
- Dongyun Lee, "Microbial Population Dynamics and Temperature Changes during Fermentation of Kimjang Kimchi", The Journal of Microbiology, 46(5), pp.590~593 (2008), 한국미생물학회
- Lee. KW, "Probiotic properties of Pediococcus strains isolated from jeotgals, salted and fermented Korean sea-food", Anaerobe, 28, pp.199~206 (2014)
- 박건영, "Health Benefits of Kimchi (Korean Fermented Vegetables) as a Probiotic Food", Journal of Medicinal Food, 17(1), pp.6~20 (2014), 한국식품영양과학회[DOI]
- 이모은, "Starter Cultures for Kimchi Fermentation", Journal of Microbiology and Biotechnology, 25(5), pp.559~568 (2015), 한국미생물·생명공학회[DOI]
- 전은비, "Characterization of Lactobacillus plantarum Lb41, an isolate from kimchi and its application as a probiotic in cottage cheese", Food Science and Biotechnology, 25(4), pp.1129~1133 (2016), 한국식품과학회[DOI]
- Jiang M, "Evaluation of probiotic properties of Lactobacillus plantarum WLPL04 isolated from human breast milk", J. Dairy Sci, 99, pp.1736~1746 (2016)
- Lee NK, "Multifunctional effect of probiotic Lactococcus lactis KC24 isolated from kimchi", LWT-Food Sci. Technol, 64, pp.1036~1041 (2015)
- CLSI, "Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement", Clin. Lab. Standards Institute, 32, pp.44~49 (2012)
- 양서진, "Antioxidant and immune-enhancing effects of probiotic Lactobacillus plantarum 200655 isolated from kimchi", Food Science and Biotechnology, 28(2), pp.491~499 (2019), 한국식품과학회[DOI]
- Kachouri F, "Involvement of antioxidant activity of Lactobacillus plantarum on functional properties of olive phenolic compounds", J. Food Sci. Technol, 52, pp.7924~7933 (2015)
- 한경준, "Anticancer and Anti-Inflammatory Activity of Probiotic Lactococcus lactis NK34", Journal of Microbiology and Biotechnology, 25(10), pp.1697~1701 (2015), 한국미생물·생명공학회[DOI]
- Son SH, "Antioxidant and immunostimulatory effect of potential probiotic Lactobacillus paraplantarum SC61 isolated from Korean traditional fermented food, jangajji", Microb. Pathog, 125, pp.486~492 (2018)
- Anukam KC, "Bile and acid tolerance of Lactobacillus plantarum KCA-1 : a potential probiotical agent", Int. J. Dairy Sci, 2, pp.275~280 (2007)
- De Vrese M, "Probiotics—compensation for lactase insufficiency", Am. J. Clin. Nutr, 73, pp.421~429 (2001)
- Vasiljevic T, "Production of β-galactosidase for lactose hydrolysis in milk and dairy products using thermophilic lactic acid bacteria", Innov. Food Sci. Emerg. Technol, 2, pp.75~85 (2001)
- Dabek M, "Distribution of β-glucosidase and β-glucuronidase activity and of β-glucuronidase gene gus in human colonic bacteria", FEMS Microbiol. Ecol, 66, pp.487~495 (2008)
- Coppola R, "Antibiotic susceptibility of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese", Dairy Sci. Tech, 85, pp.193~204 (2005)
- Monteagudo-Mera A, "Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health", Appl. Microbiol. Biotechnol, 103, pp.6463~6472 (2019)
- 송명욱, "Probiotic and Antioxidant Properties of Novel Lactobacillus brevis KCCM 12203P Isolated from Kimchi and Evaluation of Immune- Stimulating Activities of Its Heat-Killed Cells in RAW 264.7 Cells", Journal of Microbiology and Biotechnology, 29(12), pp.1894~1903 (2019), 한국미생물·생명공학회[DOI]
- Jian Zhang, "Potential Probiotic Characterization of Lactobacillus plantarum Strains Isolated from Inner Mongolia “Hurood” Cheese", Journal of Microbiology and Biotechnology, 24(2), pp.225~235 (2014), 한국미생물·생명공학회[DOI]
- Ren D, "In vitro evaluation of the probiotic and functional potential of Lactobacillus strains isolated from fermented food and human intestine", Anaerobe, 30, pp.1~10 (2014)
- 장혜지, "Probiotic characterization of Lactobacillus brevis KU15153 showing antimicrobial and antioxidant effect isolated from kimchi", Food Science and Biotechnology, 28(5), pp.1521~1528 (2019), 한국식품과학회[DOI]
- Oh NS, "Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces", PLoS One, 13, pp.e0192021~ (2018)
- 유형석, "Anti-Inflammatory Potential of Probiotic Strain Weissella cibaria JW15 Isolated from Kimchi through Regulation of NF-κB and MAPKs Pathways in LPS-Induced RAW 264.7 Cells", Journal of Microbiology and Biotechnology, 29(7), pp.1022~1032 (2019), 한국미생물·생명공학회[DOI]
