Croton hirtus L'Hér Extract Prevents Inflammation in RAW264.7 Macrophages Via Inhibition of NF-κB Signaling Pathway
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초록
Consumption of anti-inflammatory nutraceuticals may help treat or prevent inflammation-related illnesses such as diabetes, cardiovascular disease, and cancer. This study evaluated the effect of Croton hirtus L'Hér extract (CHE) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor kappa-B (NF-κB) signaling cascades. CHE significantly suppressed LPS-induced NO production and inducible nitric oxide synthase (iNOS) expression in RAW264.7 macrophages, although cyclooxygenase (COX)-2 expression was not affected. CHE also suppressed LPS-induced IκB kinase (IKK), IκB, and p65 phosphorylation in RAW264.7 cells. Western blot and immunofluorescence assays of cytosol and nuclear p65 and the catalytic subunit of NF-κB showed that CHE suppressed LPS-induced p65 translocation from the cytosol to the nucleus. CHE also suppressed LPS-induced Interleukin (IL)-6 and tumor necrosis factor (TNF)-α production in RAW264.7 cells. These results suggest that CHE prevents NO-mediated inflammation by suppressing NF-κB and inflammatory cytokines.
Abstract
Consumption of anti-inflammatory nutraceuticals may help treat or prevent inflammation-related illnesses such as diabetes, cardiovascular disease, and cancer. This study evaluated the effect of Croton hirtus L'Hér extract (CHE) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production and nuclear factor kappa-B (NF-κB) signaling cascades. CHE significantly suppressed LPS-induced NO production and inducible nitric oxide synthase (iNOS) expression in RAW264.7 macrophages, although cyclooxygenase (COX)-2 expression was not affected. CHE also suppressed LPS-induced IκB kinase (IKK), IκB, and p65 phosphorylation in RAW264.7 cells. Western blot and immunofluorescence assays of cytosol and nuclear p65 and the catalytic subunit of NF-κB showed that CHE suppressed LPS-induced p65 translocation from the cytosol to the nucleus. CHE also suppressed LPS-induced Interleukin (IL)-6 and tumor necrosis factor (TNF)-α production in RAW264.7 cells. These results suggest that CHE prevents NO-mediated inflammation by suppressing NF-κB and inflammatory cytokines.
참고문헌 (38)
- Kauppinen A, "Antagonistic crosstalk between NF-kappaB and SIRT1 in the regulation of inflammation and metabolic disorders", Cell Signal, 25, pp.1939~1948 (2013)
- Okabe Y, "Tissue biology perspective on macrophages", Nat. Immunol, 17, pp.9~17 (2016)
- Rosadini CV, "Early innate immune responses to bacterial LPS", Curr. Opin. Immunol, 44, pp.14~19 (2017)
- Shi J, "Inflammatory caspases are innate immune receptors for intracellular LPS", Nature, 514, pp.187~192 (2014)
- Lu G, "Myeloid cell-derived inducible nitric oxide synthase suppresses M1macrophage polarization", Nat. Commun, 6, pp.6676~ (2015)
- Sharma JN, "Role of nitric oxide in inflammatory diseases", Inflammopharmacology, 15, pp.252~259 (2007)
- Park MH, "Roles of NF-kappaB in cancer and inflammatory diseases and their therapeutic approaches", Cells, 5, pp.15~ (2016)
- Sun SC, "The non-canonical NF-kappaB pathway in immunity and inflammation", Nat. Rev. Immunol, 17, pp.545~558 (2017)
- Guha M, "LPS induction of gene expression in human monocytes", Cell Signal, 13, pp.85~94 (2001)
- Berry PE, "Molecular phylogenetics of the giant genus croton and tribe crotoneae(Euphorbiaceae sensu stricto)using ITS and TRNL-TRNF DNA sequence data", Am. J. Bot, 92, pp.1520~1534 (2005)
- Salatino A, "Traditional uses, chemistry and pharmacology of Croton species(Euphorbiaceae)", J. Braz. Chem. Soc, 18, pp.11~33 (2007)
- Soromou LW, "Regulation of inflammatory cytokines in lipopolysaccharidestimulated RAW 264. 7 murine macrophage by 7-O-methyl-naringenin", Molecules, 17, pp.3574~3585 (2012)
- Buchanan MM, "Toll-like receptor 4 in CNS pathologies", J. Neurochem, 114, pp.13~27 (2010)
- Ryo A, "Regulation of NF-kappaB signaling by Pin1-dependent prolyl isomerization and ubiquitin-mediated proteolysis of p65", RelA, 2003, pp.12, 1413~1426
- Luyendyk JP, "Genetic analysis of the role of the PI3K-Akt pathway in lipopolysaccharide-induced cytokine and tissue factor gene expression in monocytes", macrophages, 2008, pp.180, 4218~4226
- Cencic A, "The role of functional foods, nutraceuticals, and food supplements in intestinal health", Nutrients, 2, pp.611~625 (2010)
- Sadhukhan P, "Nutraceuticals : An emerging therapeutic approach against the pathogenesis of Alzheimer’s disease", Pharmacol. Res, 129, pp.100~114 (2018)
- Bogdan C, "Nitric oxide and the immune response", Nat. Immunol, 2, pp.907~916 (2001)
- Fang ZJ, "Cycloartane triterpenoids from Actaea vaginata with antiinflammatory effects in LPS-stimulated RAW264. 7 macrophages", Phytochemistry, 160, pp.1~10 (2019)
- Jung S, "Anti-inflammatory effects of high hydrostatic pressure extract of mulberry(Morus alba)fruit on LPS-stimulated RAW264. 7 Cells", Molecules, 24, pp.1425~ (2019)
- Lee J, "Protective effect of Tremella fuciformis Berk extract on LPS-induced acute inflammation via inhibition of the NF-kappaB and MAPK pathways", Food Funct, 7, pp.3263~3272 (2016)
- Song KM, "High yield ultrasonication extraction method for Undaria pinnatifida sporophyll and its anti-inflammatory properties associated with AP-1 pathway suppression", Lwt-Food Sci. Technol, 64, pp.1315~1322 (2015)
- Beck PL, "Inducible nitric oxide synthase from bone marrow-derived cells plays a critical role in regulating colonic inflammation", Gastroenterology, 132, pp.1778~1790 (2007)
- D'Alessio FR, "Resolution of experimental lung injury by monocyte-derived inducible nitric oxide synthase", J. Immunol, 189, pp.2234~2245 (2012)
- Pereira-Leite C, "Nonsteroidal anti-inflammatory therapy : a journey toward safety", Med. Res. Rev, 37, pp.802~859 (2017)
- Jeong YE, "Anti-inflammatory activity of populus deltoides leaf extract via modulating NF-kappaB and p38", JNK pathways, 2018, pp.19, 3746~
- Park JY, "Herbal formula SC-E1 suppresses lipopolysaccharide-stimulated inflammatory responses through activation of Nrf2", HO-1 signaling pathway in RAW 264.7 macrophages, 2017, pp.17(1), 374~
- Morgan MJ, "Crosstalk of reactive oxygen species and NF-kappaB signaling", Cell Res, 21, pp.103~115 (2011)
- Hayden MS, "NF-kappaB, the first quarter-century : remarkable progress and outstanding questions", Genes Dev, 26, pp.203~234 (2012)
- Christian F, "The regulation of NF-kappaB subunits by phosphorylation", Cells, 5, pp.12~ (2016)
- Giridharan S, "Mechanisms of NF-kappaB p65 and strategies for therapeutic manipulation", J. Inflamm. Res, 11, pp.407~419 (2018)
- Wuertz K, "Inflammatory and catabolic signalling in intervertebral discs : the roles of NF-kappaB and MAP kinases", Eur. Cell Mater, 23, pp.103~120 (2012)
- Peroval MY, "A critical role for MAPK signalling pathways in the transcriptional regulation of toll like receptors", PLoS One, 8, pp.e51243~ (2013)
- Ma JQ, "Quercetin protects mouse liver against CCl(4)-induced inflammation by the TLR2", 4 and MAPK, Int. Immunopharmacol, pp.2015, 28[DOI]
- Wang XM, "Discovering cytokines as targets for chemotherapy-induced painful peripheral neuropathy", Cytokine, 59, pp.3~9 (2012)
- Zhang JM, "Cytokines, inflammation, and pain", Int. Anesthesiol. Clin, 45, pp.27~37 (2007)
- Chaudhry H, "Role of cytokines as a double-edged sword in sepsis", In Vivo, 27, pp.669~684 (2013)
- Shim DW, "Lysimachia clethroides Duby extract attenuates inflammatory response in RAW264.7 macrophages stimulated with lipopolysaccharide and in acute lung injury mouse model", J. Ethnopharmacol., 150, pp.1007~1015 (2013)