난소를 절제한 rats에서 폐경증후군에대한 석류추출물의 효과
The effect of pomegranate on postmenopausal syndrome in ovariectomized rats
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Objective: To determine whether or not pomegranate extract can affect postmenopausal syndrome in ovariectomized rats. Methods: Seventy-nine female Sprague-Dawley rats were used: A, no intervention; B, sham operation and distilled water; C, ovariectomy and distilled water; D, ovariectomy and 10% dilute pomegranate; E, ovariectomy and 20% pomegranate; and F, ovariectomy and 40% pomegranate. The study samples were obtained 4 weeks later. The following parameters were investigated for analyses: the thickness of urogenital epithelium, tail skin temperature, serum levels of sex hormones, lipid profile, homocysteine, and thiobarbituric acid reactive substances. Results: 20% pomegranate had a transient thickening effect of bladder epithelial layer. There was an intermittent lowering effect of tail skin temperature of pomegranate extract, however, it was not consistent. There were lowering effect in serum homocysteine and elevating effect in serum high density lipoprotein-cholesterol. Pomegranate did not reverse post-ovariectomy reduced sex hormone levels. Conclusion: Pomegranate extract showed a tendency to reverse ovariectomy-related urothelial changes. Also pomegranate had a serum homocysteine-lowering effect.
Abstract
목적: 난소를 절제한 폐경모델 rats에서 석류추출물이 폐경증후군에 대해 효과가 있는지 알아보고자 하였다. 연구 방법: 79마리의 암컷 rats을 이용하였다: A, 난소 절제하지 않고 약물 투여하지 않은 군; B, 가장수술 후 증류수 투여군; C, D, E, F는 각각 난소 절제 후 증류수, 10%, 20%, 40% 석류 투여군. 약물 투여 4주째에 각 개체를 대상으로 실험을 진행하였다. 비뇨생식기 상피세포층의 수, 꼬리온도, 혈청 성호르몬, 지질대사, homocysteine, thiobarbituric acid reactive substances를 측정하였다. 결과: 20% 석류에서 방광상피세포층수의 유의한 증가가 관찰되었다. 간헐적으로 석류추출물이 쥐꼬리온도를 낮추었으나 일관된 효과를 발견하기 힘들었다. 석류추출물은 혈청 homocysteine의 감소 효과를 보여주었다. 또한 혈중 고밀도 지단백을 증가시켰다. 결론: 일부 석류추출물은 난소를 절제한 rats에서 방광상피세포의 변화를 회복시켰다. 또한 혈중 고밀도 지단백 증가 및 homocysteine 감소 효과를 관찰할 수 있었다.
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