Anti-climacterium Effects of Gagamguibiondam-tang in Ovariectomized Rats
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Purpose: The object of this study was to observe the anti-climacterium activity of Gagamguibiondam-tang (GGOT) on ovariectomized (OVX) rats, a well-documented rodent models resembles with women postmenopausal climacterium symptoms, as including cardiovascular diseases, obesity, hyperlipidemia, osteoporosis, organ steatosis and mental disorders. Methods: In this study, anti-climacteric effects were evaluated separated into three categories; 1) anti-obese, 2) anti-uterine atrophy and 3) anti-osteoporotic effects. Five groups were used (8 rats in each group); sham control, OVX control, GGOT 500, 250 and 125 mg/kg administered groups. Twenty-eight days after bilateral OVX surgery, GGOT were orally administered, once a day for 84 days, and then the changes on the body weight and gain during experimental periods, serum estradiol levels, abdominal fat pad and uterus weights with histopathology of abdominal fat pads (total thickness and mean adipocyte diameters) and uterus (total, epithelial and mucosal thickness, percentages of uterine gland regions) for anti-obese and estrogenic effects. In addition, femur, tibia and fourth or fifth lumbar vertebrae (L4 or L5) wet, dry and ash weights, mineral density (BMD), bone strength (failure load), serum osteocalcin and bone specific alkaline phosphatase (bALP) contents, histological and histomorphometrical analyses - bone mass and structure with bone resorption, were monitored for anti-osteoporosis activity. Results: As a result of OVX, noticeable increases of body weight and gains, food and water consumption, weights of abdominal fat pad deposited in dorsal abdominal cavity, serum osteocalcin levels were demonstrated in this experiment with decrease of uterus, femur, tibia and L5 weights, serum bALP and estradiol levels. In addition, marked hypertrophic changes of adipocytes located in deposited abdominal fat pads, uterine disused atrophic changes, decreases of bone mass and structures of femur, tibia and L4 were also observed in OVX control rats with dramatic increases of bone resorption markers, the Ocn and OS/BS at histopathological and histomorphometrical analysis in this study as compared with sham-operated control rats, suggesting the estrogen-deficient climacterium symptoms - obese and osteoporosis were induced by OVX, respectively. However, these estrogen- deficient climacterium symptoms induced by bilateral OVX in rats were significantly inhibited by 84 days of continuous oral treatment of GGOT 500, 250 and 125 mg/kg, respectively. Especially, GGOT 500, 250 and 125 mg/kg showed clear dose-dependent inhibitory activities on the OVX-induced climacterium signs. Conclusion: The results suggest that oral administration of GGOT 500, 250 and 125 mg/kg has clear dose-dependent favorable anti-climacterium effects - estrogenic, anti-obese and anti-osteoporotic activities in OVX rats in this experiment.
Abstract
Purpose: The object of this study was to observe the anti-climacterium activity of Gagamguibiondam-tang (GGOT) on ovariectomized (OVX) rats, a well-documented rodent models resembles with women postmenopausal climacterium symptoms, as including cardiovascular diseases, obesity, hyperlipidemia, osteoporosis, organ steatosis and mental disorders. Methods: In this study, anti-climacteric effects were evaluated separated into three categories; 1) anti-obese, 2) anti-uterine atrophy and 3) anti-osteoporotic effects. Five groups were used (8 rats in each group); sham control, OVX control, GGOT 500, 250 and 125 mg/kg administered groups. Twenty-eight days after bilateral OVX surgery, GGOT were orally administered, once a day for 84 days, and then the changes on the body weight and gain during experimental periods, serum estradiol levels, abdominal fat pad and uterus weights with histopathology of abdominal fat pads (total thickness and mean adipocyte diameters) and uterus (total, epithelial and mucosal thickness, percentages of uterine gland regions) for anti-obese and estrogenic effects. In addition, femur, tibia and fourth or fifth lumbar vertebrae (L4 or L5) wet, dry and ash weights, mineral density (BMD), bone strength (failure load), serum osteocalcin and bone specific alkaline phosphatase (bALP) contents, histological and histomorphometrical analyses - bone mass and structure with bone resorption, were monitored for anti-osteoporosis activity. Results: As a result of OVX, noticeable increases of body weight and gains, food and water consumption, weights of abdominal fat pad deposited in dorsal abdominal cavity, serum osteocalcin levels were demonstrated in this experiment with decrease of uterus, femur, tibia and L5 weights, serum bALP and estradiol levels. In addition, marked hypertrophic changes of adipocytes located in deposited abdominal fat pads, uterine disused atrophic changes, decreases of bone mass and structures of femur, tibia and L4 were also observed in OVX control rats with dramatic increases of bone resorption markers, the Ocn and OS/BS at histopathological and histomorphometrical analysis in this study as compared with sham-operated control rats, suggesting the estrogen-deficient climacterium symptoms - obese and osteoporosis were induced by OVX, respectively. However, these estrogen- deficient climacterium symptoms induced by bilateral OVX in rats were significantly inhibited by 84 days of continuous oral treatment of GGOT 500, 250 and 125 mg/kg, respectively. Especially, GGOT 500, 250 and 125 mg/kg showed clear dose-dependent inhibitory activities on the OVX-induced climacterium signs. Conclusion: The results suggest that oral administration of GGOT 500, 250 and 125 mg/kg has clear dose-dependent favorable anti-climacterium effects - estrogenic, anti-obese and anti-osteoporotic activities in OVX rats in this experiment.
참고문헌 (37)
- The Society of Korean Medicine Obstetrics and Gynecology, "Korean Obstetrics &Gynecology Ⅰ", pp.237~238 (2007), Jungdam publisher
- del Giorno C, "Effects of Trifolium pratense on the climacteric and sexual symptoms in postmenopause women", Rev Assoc Med Bras, 56(5), pp.558~562 (2010)
- Liang YQ, "Estrogen receptor βis involved in the anorectic action of estrogen", Int J Obes Relat Metab Disord, 26(8), pp.1103~1109 (2002)
- Saengsirisuwan V, "Modulation of insulin resistance in ovariectomized rats by endurance exercise training and estrogen replacement", Metabolism, 58(1), pp.38~47 (2009)
- Lew YO, "Side effects and management of postmenopausal hormone replacement therapy", J Menopausal Med, 10(1), pp.14~20 (2004)
- Kim KS, "Literary Study on the Climacteric Syndrome", J Oriental medical laboratory of Daejeon univ, 13(1), pp.107~128 (2004)
- Heo J., "Dongeuibogam (東醫寶鑑)", pp.47~ (2008), Hanmibook
- Cha YJ, "The Effect of Guibiondamtang in an Animal Model of Depression using Chronic Mild Stress", J Oriental Neuropsychiatry, 12(2), pp.53~68 (2001)
- Whang WW, "The Effect of Guibiondamtang on Immune Reponse and in Concentration of Catecholamine in Immobilization Stressed Rats", J Oriental Neuropsychiatry, 6(1), pp.1~17 (1995)
- Kang SJ, "Dried pomegranate potentiates anti-osteoporotic and antiobesity activities of red clover dry extracts in ovariectomized rats", Nutrients, 7(4), pp.2622~2647 (2015)
- Levene A., "Pathological factors influencing excision of tumours in the head and neck. Part I", Clin Otolaryngol Allied Sci, 6(2), pp.145~151 (1981)
- Ludbrook J., "Update: microcomputer statistics packages. A personal view", Clin Exp Pharmacol Physiol, 24(3-4), pp.294~296 (1997)
- Kang SJ, "Fermentation with Aquilariae Lignum enhances the antidiabetic activity of green tea in type II diabetic db", db mouse, 2014, pp.6(9), 3536~3571
- Wolff LPG, "Avaliação do endométrio em menopausadas após uso de isoflavonas", Rev Assoc Med Bras, 52(6), pp.419~423 (2006)
- Choi JS, "Genistein reduced insulin resistance index through modulating lipid metabolism in ovariectomized rats", Nutr Res, 32(11), pp.844~855 (2012)
- Tohda C, "Inhibitory effects of Eleutherococcus senticosus extracts on amyloid beta(25-35)-induced neuritic atrophy and synaptic loss", J Pharmacol Sci, 107(3), pp.329~339 (2008)
- Nakagawasai O, "Effect of kamiuntan-to on the impairment of learning and memory induced by thiaminedeficient feeding in mice", Neuroscience, 125(1), pp.233~241 (2004)
- Isabelle Busta, "The Use of Gui-Pi-Tang in Small Animals with Immune-Mediated Blood Disorders", 한국임상수의학회지, 26(2), pp.181~184 (2009), 한국임상수의학회
- Kang IH, "Effects of Gwibitang on glutamateinduced apoptosis in C6glial cells", J Korean Orient Med, 22(4), pp.45~57 (2001)
- Yim NH, "Guibitang, a traditional herbal medicine, induces apoptotic death in A431 cells by regulating the activities of mitogen-activated protein kinases", BMC Complement Altern Med, 14, pp.344~ (2014)
- 김학재, "스트레스와 에탄올로 유발된 mouse의 위점막 손상에 대한 귀비탕의 예방효과", 대한한의학회지, 24(1), pp.155~168 (2003), 대한한의학회
- Wang Q, "Potentiation of brain acetylcholine neurons by Kami-Untan-To(KUT)in aged mice : implications for a possible antidementia drug", Phytomedicine, 7(4), pp.253~258 (2000)
- Nakagawasai O, "Preventive effect of kami-untan-to on performance in the forced swimming test in thiaminedeficient mice : relationship to functions of catecholaminergic neurons", Behav Brain Res, 177(2), pp.315~321 (2007)
- Oh HK, "Kami-ondam-tang, a traditional herbal prescription, attenuates the prepulse inhibition deficits and cognitive impairments induced by MK-801 in mice", J Ethnopharmacol, 146(2), pp.600~607 (2013)
- Hong JG, "The memory-enhancing effects of Kami-ondam-tang in mice", J Ethnopharmacol, 137(1), pp.251~256 (2011)
- Geary N., "The estrogenic inhibition of eating. Handbook of behavioral neurobiology. Vol.14", pp.307~345 (2004), Kluwer Academic
- Eckel LA, "Estradiol : a rhythmic, inhibitory, indirect control of meal size", Physiol Behav, 82(1), pp.35~41 (2004)
- Lutz TA, "Amylin may offer(more)help to treat postmenopausal obesity", Endocrinology, 152(1), pp.1~3 (2011)
- Korach KS, "Principles of Pharmacology:Basic Concepts and Clinical Applications", pp.809~825 (1995), Chapman and Hall
- Ateba SB, "Eriosema laurentii De Wild(Leguminosae)methanol extract has estrogenic properties and prevents menopausal symptoms in ovariectomized Wistar rats", J Ethnopharmacol, 150(1), pp.298~307 (2013)
- Sakai A, "Bone marrow cell development and trabecular bone dynamics after ovariectomy in ddy mice", Bone, 23(5), pp.443~451 (1998)
- Wronski TJ, "Estrogen and diphosphonate treatment provide long-term protection against osteopenia in ovariectomized rats", J Bone Miner Res, 6(4), pp.387~394 (1991)
- Puel C, "Preventive effect of Abelmoschus manihot (L.) Medik. on bone loss in the ovariectomised rats", J Ethnopharmacol, 99(1), pp.55~60 (2005)
- Kuo YJ, "Calcitonin inhibits SDCP-induced osteoclast apoptosis and increases its efficacy in a rat model of osteoporosis", PLoS One, 7(7), pp.e40272~ (2012)
- Syed Z, "Bone densitometry : applications and limitations", J Obstet Gynaecol Can, 24(6), pp.476~484 (2002)
- Yamaguchi K, "Suppressive effect of norzoanthamine hydrochloride on experimental osteoporosis in ovariectomized mice", Biol Pharm Bull, 22(9), pp.920~924 (1999)
- Jakubas-Przewlocka J, "Assessment of changes due to the long-term effect of estrogen and calcium deficiency in the trabecular bone structure in rats", Clin Exp Rheumatol, 23(3), pp.385~388 (2005)