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Prevalence and Molecular Epidemiology of Vancomycin-Resistant Enterococci (VRE) Strains Isolated from Animals and Humans in Korea

Hee Jin Cheong|Woo Joo Kim|Won Ki Bae|Joon Young Song|In Sook Hwang|Joong Sik Eom|ong Ho Park
대한내과학회 (The Korean Association Of Internal Medicine)|2005.03|The Korean Journal of Internal Medicine|제20권 제1호|pp. 55-62

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Abstract

Background : To assess the possibility of VRE transmission from animals to humans, we studied the prevalence of vancomycin-resistant enterococci (VRE) in farm animals, raw chicken meat, and healthy people. We then determined the molecular relatedness of VRE isolates between animals and humans in Korea. Methods : We aimed to isolate VRE from 150 enterococci specimens of farm animals, 15 raw chicken meat samples, and stools from 200 healthy people. Species differentiation was done with conventional biochemical tests. Vancomycin resistance genotyping was done by polymerase chain reaction (PCR). Using the agar dilution method, antimicrobial susceptibility was tested for 8 antimicrobials and pulsed-field gel electrophoresis (PFGE) was done to evaluate the molecular relatedness of VRE isolates. Results : The prevalence of VRE was 14.7% (22/150) in farm animal specimens, 1% (2/200) in healthy people, and 60% (9/15) in raw chicken meat. Of 22 animal VRE isolates, 1 vanA E. faecium, 15 vanC1 E. gallinarum, and 6 vanC2 E. casseliflavus were identified. All of the 9 VRE from raw chicken meat and all of the 20 clinical VRE strains were vanA E. faecium. However, in healthy people, only 2 vanC2 E. casseliflavus were isolated. These showed low-level resistance to vancomycin and susceptibility to teicoplanin. However, 9 VRE strains from raw chicken meat had high-level resistance to vancomycin (MIC50,90: >128 μg/mL), teicoplanin (MIC50,90: >128 μg/mL), ampicillin (MIC50,90: >128 μg/mL), erythromycin (MIC50,90: >128 μg/mL), and tetracycline (MIC50/90: 128/>128 μg/mL). Conclusion : This study demonstrated little evidence of VRE colonization in healthy people despite high recovery of VRE among raw chicken meat. It is suggested that there is little evidence of VRE transmission from animals to healthy people. However, we assumed that there exists the possibility of VRE contamination during the processing of chicken meat.

참고문헌 (30)

  1. "The life and times of the enterococcus", 3, pp.46~65 (1990)
  2. "Major trends in the microbial etiology of nosocomial infection", pp.3~ (1991)
  3. "Plasmid-mediated resistance to vancomycin and teicoplanin in enterococcus faecium", 319, pp.157~161 (1988)
  4. "The epidemiology of vancomycin-resistant enterococci", 12, pp.537~541 (1999)
  5. "Vancomycin-resistant enterococci in the regional society", 13, pp.37~41 (2002)
  6. "Development of multiplex PCR for detection of vancomycin-resistant enterococci and epidemiological application in Korea", 17, pp.369~384 (1999)
  7. "Performance standards for antimicrobial susceptibility testing: 9th international supplement NCCLS document", 19, pp.100~109 (1999)
  8. "Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis:criteria for bacterial strain typing", 33, pp.2233~2239 (1995)
  9. "Global epidemiology of glycopeptide-resistant enterococci Proceedings of 3rd International Symposium on Antimicrobial Agents and Resistance", pp.129~139 (2001), Seoul
  10. Sundssfjord A, "Human infections caused by glycopeptide-resistant enterococcus spp.: are they a zoonosis?", 7, pp.16~33 (2001)
  11. "Influence of oral glycopeptides on the fecal flora of human volunteers:selection of highly glycopeptide-resistant enterococci", 173, pp.1129~1136 (1996)
  12. "A molecular epidemiologic study on vancomycin-resistant enterococci isolated from clinical specimens over a 5-year period", 32, pp.203~211 (2000)
  13. "Comparison of direct plating and broth enrichment culture for the detection of intestinal colonization by glycopeptide-resistant enterococci among hospitalized patients", 37, pp.1436~1440 (1999)
  14. "Vancomycin- resistant enterococci isolated from animals and food", 54, pp.197~204 (2000)
  15. "Community dissemination of vancomycin-resistant Enterococcus faecium", 29, pp.316~320 (2001)
  16. "Cost-effective algorithm for detection and identification of vancomycin-resistant enterococci in surveillance cultures", 19, pp.366~369 (2000)
  17. "method to detect hetero-resistance to vancomycin in Staphylococcus aureus in a UK hospital", pp.399~403 (2001)
  18. "Molecular relationship of vanA glycopeptide resistance gene in enterococci from hospitalized patients and poultry in Korea", 33, pp.383~391 (2001)
  19. "Vancomycin- resistant enterococci in turkey and farmers", 337, pp.1558~1559 (1997)
  20. "Study on the infection due to VanA type vancomycin-resistant enterococci", 30, pp.10~18 (1998)
  21. "Rectal surveillance culture of vancomycin-resistant enterococci colonization among patients hospitalized in the intensive care unit", 31, pp.203~209 (1999)
  22. "Nationwide questionnaire survey for the prevalence, detection methods, and infection control program of VRE among hospitals. Korean", 33, pp.78~87 (2001)
  23. "Genetics and mechanisms of glycopeptide resistance in enterococci", 37, pp.1563~1571 (1993)
  24. "Antibiotic resistance of faecal enterococci in poultry, poultry farmers and poultry slaughterers", 49, pp.497~505 (2002)
  25. "Antimicrobial resistance:relation to human and animal exposure to antibiotics", 40, pp.453~454 (1997)
  26. "Vancomycin-resistant enterococcal infection", 342, pp.710~721 (2000)
  27. "Susceptibi1ity tests of vancomycin-resistant enterococci", 24, pp.133~137 (1992)
  28. "Vancomycin-resistant enterococcal bacteremia:natural history and attributable mortality", 23, pp.1234~1239 (1996)
  29. "The changing molecular epidemiology and establishment of endemicity of vancomycin resistant enterococci at one hospital over a 6-year period", 179, pp.163~171 (1999)
  30. "Characterization of glycopeptide-resistant enterococci from U", te(ver fc), pp.2311~2317 (1993)

관련 논문AI추천

국내 Avoparcin 사용 금지 후 가축과 사람에서 반코마이신 내성 장구균의 분리 빈도 및 유전적 연관성곽효선, 김봉수, 안재오, 유재일, 이영선, 이점규, 이하림, 정영희대한감염학회 · Infection and Chemotherapy2006 · 제38권 제1호Resistance Mechanism and Epidemiology of Vancomycin-resistant Enterococci이위교대한임상미생물학회 · Annals of Clinical Microbiology2008 · 제11권 제2호일개 대학병원 유래의 VanA 형 Vancomycin 내성 장구균에서의 vanA Gene Cluster의 다양성김정민, 윤정중, 이남용, 이지향대한감염학회 · Infection and Chemotherapy2003 · 제35권 제6호PCR에 의한 vancomycin 내성 장구균의 유전자형 동정김신무, 성치남, 심은숙대한임상검사과학회 · 대한임상검사과학회지1998 · 제30권 제3호물 환경 시료에서 반코마이신 내성 엔테로코커스(VRE) 검출을 위한 Multiplex PCR의 적용성 연구Bo-Hyun Lim, Gi Don Kim, Min-jeong Kim, Sang Tae Lee, Se Hee Nam, Soon Bok Kwon, Yong Yeon Kim한국환경분석학회 · 환경분석과 독성보건2009 · 제12권 제3호Further Increase of Vancomycin-Resistant Enterococcus faecium, Amikacin- and Fluoroquinolone-Resistant Klebsiella pneumoniae, and Imipenem-Resistant Acinetobacter spp. in Korea박기형, 신종희, 용동은, 이경원, 임환섭, 정석훈, 정윤섭, 하경임연세대학교의과대학 · Yonsei Medical Journal2006 · 제47권 제1호

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