생쥐 소교세포(BV-2)에서 우르소데옥시콜린산에 의한 iNOS 발현억제
Inhibition of iNOS Expression Via Ursodeoxycholic Acid in Murine Microglial Cell, BV-2 Cell Line
2
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
Background: Inflammation in the brain has known to be associated with the development of a various neurological diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide (NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease (AD) which is resulted in cell death. Among those pro-inflammatory compounds, NO contributes to the cell death by directly or indirectly. Methods: In the study, we examined whether ursodeoxycholic acid (UDCA), a non-toxic hydrophilic bile acid, inhibits the NO production by a direct method using Griess reagent and by RT-PCR in the gene expression of inducible nitric oxide synthase (iNOS). In signal transduction, we also examined the NF-κB (p65/p50), IKK, and IκB, which are associated with the expression of iNOS gene using western blots. Results: In the present study, we found that UDCA effectively inhibited NO production in BV-2 microglial cell, and NF-κB activation was reduced by suppressing IKK gene expression and by increasing the IκB in cytosol comparing those to the positive control LPS. Conclusion: Taken together, these data suggested that UDCA may play a crucial role in inhibiting the NO production and the results imply that UDCA suppresses a cue signal of the microglial activation via stimulators, such asβ-amyloid peptides which are known to stimulate microglia in AD pathogenesis.
참고문헌 (18)
- "NF-kappaB in transplantation friend or foe. Transpl Infect Dis", 3, pp.212~219 (2001)
- "Inhibition of nitric oxide synthase as a potential therapeutic target. Annu Rev Pharmacol Toxicol. 39", 39, pp.191~220 (1999)
- "Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett 571", 571, pp.50~54 (2004)
- "key regulators of the NF-kappaB pathway. Trends Biochem Sci", 29, pp.72~79 (2004)
- "a sensor for pathological events in the CNS. Trends Neurosci", 19, pp.312~318 (1996)
- "but not lipopolysaccharide-mediated induction of nitric oxide and tumor necrosis factor-alpha in microglia. J Neuroimmunol", 151, pp.85~93 (2004), Sodium butyrate suppresses interferon-gamma-
- "Activation of microglial cells by beta-amyloid protein and interferon-gamma. Nature", 374, pp.647~650 (1995)
- "Alzheimer's disease genes proteins", pp.741~766 (2001)
- "Oltipraz inhibits inducible nitric oxide synthase in vitro and inhibits nitric oxide production in activated microglial cells. Arch Biochem Biophys", 424, pp.163~170 (2004)
- "gene expression in activated microglia. Neuropharmacology", 47(\), pp.243~252 (2004), A new anti-inflammatory agent KL-1037 represses proinflammatory cytokine and inducible nitric oxide synthase
- "Ursodeoxycholic acid as adjunctive therapy for problematic type 1 autoimmune hepatitis", 30, pp.1381~1386 (1999)
- "Tauroursodeoxycholic acid enhances phagocytosis of the cultured rat Kupffer cell. J Gastroenterol Hepatol", 14, pp.652~658 (1999)
- "Ursodeoxycholic acid prevents cytochrome c release in apoptosis by inhibiting mitochondrial membrane depolarization and channel formation. Cell Death Differ", 6, pp.842~854 (1999)
- "IL-1 beta and nitric oxide in rat microglia. Arch Pharm Res", 26, pp.1067~1073 (2003)
- "Immortalization of murine microglial cells by a v-raf", v-myc carrying retrovirus. J Neuroimmunol, 1990, pp.27, 229~237
- "An immortalized cell line expresses properties of activated microglial cells. J Neurosci Res 31", pp.616~621 (1992)
- "Oltipraz inhibits inducible nitric oxide synthase in vitro and inhibits nitric oxide production in activated microglial cells. Arch Biochem Biophys", 424, pp.163~170 (2004)
- "Potential role of ursodeoxycholic acid in suppression of nuclear factor kappa B in microglial cell line", 27, pp.954~2 960 (2004)