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폐암의 면역세포 치료: 동물 모델에서 수지상 세포를 이용한 Adjuvant Therapy 가능성 연구

Immunocell Therapy for Lung Cancer: Dendritic Cell Based Adjuvant Therapy in Mouse Lung Cancer Model

이현아|김명주|백소영|인소희|이석재
대한면역학회 (The Korean Association of Immunologists)|2005.03|Immune Network|제5권 제1호|pp. 36-44

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Abstract

The anti-tumor therapeutic effect of autologous tumor cell lysate pulsed- dendritic cells (DCs) was studied for non-immunogenic and immune suppressive lung cancer model. To test the possibility as an adjuvant therapy, minimal residual disease model was considered in mouse in vivo experiments. Methods: Syngeneic 3LL lung cancer cells were inoculated intravenously into the C57BL/6 mouse. Autologous tumor cell (3LL) or allogeneic leukemia cell (WEHI-3) lysate pulsed-DCs were injected twice in two weeks. Intraperitoneal DC injection was started one day (MRD model) after tumor cell inoculation. Two weeks after the final DC injection, tumor formation in the lung and the tumor-specific systemic immunity were observed. Tumor-specific lymphocyte proliferation and the IFN-r secretion were analyzed for the immune monitoring. Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 for 7 days and pulsed with tumor cell lysate for 18 hrs. Results: Compared to the saline treated group, tumor formation was suppressed in 3LL tumor cell lysate pulsed-DC treated group, while 3LL-specific immune stimulation was minimum. WEHI-3-specific immune stimulation occurred in WEHI-3 lysate-pulsed DC treated group, which had no correlation with tumor regression. Conclusion: The data suggest the possible anti-tumor effect of cultured DCs as an adjuvant therapy for minimal residual disease state of lung cancer. The significance of immune modulation in DC therapy including the possible involvement of NK cell as well as antigen-specific cytotoxic T cell activity induction was discussed.

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수지상세포를 이용한 항암 면역 치료: 생쥐 신장암 모델을 이용한 연구백소영, 이현아, 이홍기, 정철원, 최광민대한면역학회 · Immune Network2004 · 제4권 제1호동종 종양 세포 용해액을 이용한 수지상 세포 항암 백신의 흑색종 폐암 전이 모델에서의 효과 연구권영도, 김명주, 백소영, 이영준, 이현아, 인소희, 최옥미대한면역학회 · Immune Network2005 · 제5권 제3호수지상세포를 이용한 편평세포암종 예방백신고예정, 손영익, 이치규, 정승원대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2004 · 제47권 제12호마우스 동종 줄기세포 유래 수지상 세포를 이용한 백신의 흑색종 폐암 전이 모델에서의 항암 효과 및 기전 연구김명주, 백소영, 손혜진, 이강은, 이영준, 이현아대한면역학회 · Immune Network2006 · 제6권 제3호종양 세포 용해액에 따른 수지상세포 유도 항원 특이 면역반응 차이의 기전 연구김명주, 백소영, 손혜진, 이강은, 이현아대한면역학회 · Immune Network2006 · 제6권 제3호수지상세포의 항원제시 능력 및 항암활성에 미치는 Lipofectin의 영향노영욱, 임종석대한면역학회 · Immune Network2006 · 제6권 제3호

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