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종양 세포 용해액에 따른 수지상세포 유도 항원 특이 면역반응 차이의 기전 연구

Mechanism of Differential Ag-specific Immune Induction by Different Tumor Cell Lysate Pulsed DC

이현아|손혜진|김명주|백소영|이강은
대한면역학회 (The Korean Association of Immunologists)|2006.06|Immune Network|제6권 제3호|pp. 145-153

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Abstract

Background: Tumor cell lysate has been considered as a preferential antigen source for the therapeutic dendritic cell pulsing. Our experiences with in vivo study with animal tumor model indicate the tumor cell lysate dependent differential effect of DC therapy. Our previous data show that MC38 lysate pulsed-DC induced stronger ag-specific immunity than CT26 lysate pulsed-DC in vitro. In this study we tried to reveal the mechanism for differential induction of ag-specific immunity of different colon cancer cell lysate pulsed-DCs. Methods: MC38 and CT26 cell lines were prepared as lysate by freezing-thawing procedure. Tumor cell antigenicity was confirmed by detecting the surface expression of MHC I/II & B7.1/2 molecules. IL-10, IL-12 and TGF-beta in the tumor cell lysate were detected by ELISA and the presence of heat shock proteins were analysed by western blotting. Results: The secretion of IL-10, a immune-inhibitory cytokine was about 470% higher in CT26 lysate than in MC38. Hsp 70 was detected only in the MC38 lysate but not in the CT26. On the other hand, Hsp 60 and 90 expression were not different in two colon cancer cell lysates. Conclusion: In two different colon caner cell lysate, immune inhibitory IL-10 (higher in CT26) and Hsp70 (MC38 superiority) were differentially expressed. These data indicate that higher ag- specific immunity induction by MC38 lysate pulsed-DC may due to the expression of hsp70 and lower secretion of IL-10, a immune-inhibitory cytokine than CT26 lysate. The significance of other cytokine and the surface marker expression will be discussed. (Immune Network 2006;6(3):145-153)

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관련 논문AI추천

수지상세포의 항원제시 능력 및 항암활성에 미치는 Lipofectin의 영향노영욱, 임종석대한면역학회 · Immune Network2006 · 제6권 제3호수지상세포를 이용한 항암 면역 치료: 생쥐 신장암 모델을 이용한 연구백소영, 이현아, 이홍기, 정철원, 최광민대한면역학회 · Immune Network2004 · 제4권 제1호동종 종양 세포 용해액을 이용한 수지상 세포 항암 백신의 흑색종 폐암 전이 모델에서의 효과 연구권영도, 김명주, 백소영, 이영준, 이현아, 인소희, 최옥미대한면역학회 · Immune Network2005 · 제5권 제3호수지상세포를 이용한 편평세포암종 예방백신고예정, 손영익, 이치규, 정승원대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2004 · 제47권 제12호폐암의 면역세포 치료: 동물 모델에서 수지상 세포를 이용한 Adjuvant Therapy 가능성 연구김명주, 백소영, 이석재, 이현아, 인소희대한면역학회 · Immune Network2005 · 제5권 제1호골수성백혈병에서 배양한 수지상세포(Dendritic Cell)에 대한 종양항원 감작법으로 IL-12 첨가와 융합법의 효과김기원, 박석영, 홍영선대한면역학회 · Immune Network2004 · 제4권 제1호

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