수지상세포를 이용한 항암 면역 치료: 생쥐 신장암 모델을 이용한 연구
Dendritic Cell Based Cancer Immunotherapy: in vivo Study with Mouse Renal Cell Carcinoma Model
2
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
Background: As a potent antigen presenting cell and a powerful inducer of antigen specific immunity, dendritic cells (DCs) are being considered as a promising anti-tumor therapeutic module. The expected therapeutic effect of DCs in renal cell carcinoma was tested in the mouse model. Established late-stage tumor therapeutic (E-T) and minimal residual disease (MRD) model was considered in the in vivo experiments. Methods: Syngeneic renal cell carcinoma cells (RENCA) were inoculated either subcutaneously (E-T) or intravenously (MRD) into the Balb/c mouse. Tumor cell lysate pulsed-DCs were injected twice in two weeks. Intraperitoneal DC injection was started 3 week (E-T model) or one day (MRD model) after tumor cell inoculation. Two weeks after the final DC injection, the tumor growth and the systemic immunity were observed . Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 for 7 days and pulsed with RENCA cell lysate for 18 hrs. Results: Compared to the saline treated group, tumor growth (E-T model) or formation (MRD model) was suppressed in pulsed-DC treated group. RENCA specific lymphocyte proliferation was observed in the RENCA tumor-bearing mice treated with pulsed-DCs. Primary cytotoxic T cell activity against RENCA cells was increased in pulsed-DC treated group. Conclusion: The data suggest the possible anti-tumor effect of cultured DCs in established or minimal residual disease/metastasis state of renal cell carcinoma. Systemic tumor specific immunity including cytotoxic T cell activity was modulated also in pulsed-DC treated group. (Immune Network 2004;4(1):44-52)
참고문헌 (37)
- "Identificaion of a novel cell type in peripheral lymphoid organs of mice. I. Morphology", pp.1142~1162 (1973)
- "Prospects for immunotherapy directed to the T cell receptor in human autoimmune disease. Ann N Y Acad Sci 636", pp.147~153 (1991)
- "Dendritic cells presenting tumor antigen.", 43, pp.158~ (1996)
- "Dendritic cells and the control of immunity.", 392, pp.245~252 (1998)
- "Dendritic cells: a link between innate and adaptive immunity.", 19, pp.12~25 (1999)
- "Linking innate and adaptive immunity.", 5, pp.868~870 (1999)
- "Direct activation of CD8+ cytotoxic T lymphocytes by dendritic cells. J Exp Med 166"
- "Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific MHC-restricted T cells in situ. J Exp Med 172", pp.631~640 (1990)
- "Murine dendritic cells pulsed in vitro with tumor antigen induce tumor resistance in vivo. Eur J Immunol 24", pp.605~610 (1994)
- "Peptide-pulsed dendritic cells induce antigen-specific, CTL- mediated protective tumor immunity.", 183, pp.283~288 (1996)
- "Dendritic cells: Unique leukocyte populations which control the primary immune response.", 90(9), pp.3245~3287 (1997)
- "Induction and clonal expansion of tumor-specific cytotoxic T lymphocytes from renal cell carcinoma patients after stimulation with autologous dendritic cells loaded with tumor cells.", 91, pp.749~ (2001)
- "Recognition of chronic myelogeneous leukemia cells by autologous T lymphocytes primed in vitro against the patient's dendritic cells.", 112(3), pp.740~748 (2001)
- "Dendritic cells directly trigger NK cell functions: Cross-talk relevant in innate anti-tumor immune responses in vivo.", 5(4), pp.405~410 (1999)
- "Metastatic renal-cell carcinoma: what causes occasional dramatic regressions?", 338(18), pp.1305~1306 (1998)
- "Annual Report of the Central Cancer Registry in Korea", pp.42~ (2001), Ministry of Health and Welfare
- "Immunosuppression in murine renal cell carcinoma.I. Characterization of extent", pp.325~334 (1990)
- "Interferon-α and survival in metastatic renal carcinoma early results of a randomized controlled trial.", 353, pp.14~ (1999)
- "A progress report on the treatment of 157 patients with advanced cancer using lymphokine-activated killer cells and interleukin-2 or high-dose interleukin-2 alone. N Engl J Med 316" (1987)
- "Immunosuppression in murine renal cell carcinoma II. Identification of responsible lymphoid cell phenotypes and examination of eliminatin of suppression. Cancer Immunol Immunothe 31", pp.335~341 (1990)
- "Phase I", II radioimmunotherapy trial with iodine-131-labeled monoclonal antibody G250 in metastatic renal cell carcinoma., 1998, pp.4, 2729~
- "Molecular cloning and immunogenicity of renal cell carcinoma-associated antigen G250.", 85, pp.865~ (2000)
- "Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte", macrophage colony-stimulating factor. J Exp Med 176, 1992, 1693~1702
- "Mechanism of regression of mammary adenocarcinomas in rats following plasma adsorption over protein A-containing staphylococcus aureus. Cancer Res 42", pp.4970~4974 (1982)
- "Phorbol ester effects on splenic lymphocyte composition and cytotoxic T cell activities of SSIN mice a strain deficient in CD8+ T cells.", 17(12), pp.2617~2624 (1996)
- "Dendritic cell based therapy of cancer. Dendritic Cells in Fundamental and Clinical Immunology." (1997), Plenum Press
- "and dendritic cells arise from a common major histocompatibility complex calss II-negative progenitor in mouse bone marrow. Proc Natl Acad Sci USA 90", pp.3038~3042 (1993)
- "Dendritic cell ontogeny: a human dendritic cell lineage of myeloid origin.", 94, pp.12551~12556 (1997)
- "Myeloid dendritic cells.", 66(2), pp.205~208 (1999)
- "Development of thymic and splenic dendritic cell populations from different hemopoietic precursors.", 98, pp.3376~3382 (2001)
- "Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte", macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor-., 1994., ~
- "Final maturation of dendritic cells id associated with impaired responsiveness to IFN-gamma and to bacterial IL-12 inducers decreased ability ofmature dendritic cells to produce IL-12 during the interaction with Th cells.", 162(6), pp.3231~3236 (1999)
- "Pro-inflammatory cytokines and prostaglandines induce maturaton of potent immunostimulatory dendritic cells under feta-calf-serum free conditions.", 27(12), pp.3135~3142 (1999)
- "Imunological and clinical responses in metastatic renal cancer patients vaccinated with tumor RNA-transfecteddendritic cells.", 63, pp.2127~2133 (2003)
- "Vaccination of patients with metastatic renal cell carcinoma with autologous dendritic cells pulsed with autologous tumor antigen in combination with interleukin-2: A phase I study.", 25(6), pp.500~508 (2002)
- "A pilot trial of tumor lysate-loaded dendritic cells for the treatment of metastatic renal cell carcinoma.", 26(5), pp.412~419 (2003)
- "Cellular and humoral immune responses in patients with metastatic renal cell carcinoma after vaccination with antigen pulsed dendritic cells.", 161, pp.777~ (1999)
