Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Silk peptide, the hydrolysate of silk protein derived from cocoons, has been employed as a biomedical material and is believed to be safe for human use. Silk peptide display various bioactivities, including anti-inflammatory, immune-regulatory, anti-tumor, anti-viral, and anti-bacterial. Although earlier investigations demonstrated that silk peptide stimulates macrophages and the production of pro-inflammatory cytokines, its effect on natural killer (NK) cell function has not yet been explored. In this study, we initially confirmed that silk peptide enhances NK cell activity in vitro and ex vivo. To assess the modulatory activity of silk peptide on NK cells, mice were fed various amounts of a silk peptide-supplemented diet for 2 months and the effects on immune stimulation, including NK cell activation, were evaluated. Oral administration of silk peptide significantly enhanced the proliferation of mitogen- or IL-2-stimulated splenocytes. In addition, oral silk peptide treatment enhanced the frequency and degree of maturation of NK cells in splenocytes. The same treatment also significantly enhanced the target cell cytolytic activity of NK cells, which was determined by cell surface CD107a expression and intracellular interferon-γ expression. Finally, oral administration of silk peptide stimulated T helper 1-type cytokine expression from splenic lymphocytes. Collectively, our results suggest that silk peptide potentiates NK cell activity in vivo and could be used as a compound for immune-modulating anti-tumor treatment.
Abstract
Silk peptide, the hydrolysate of silk protein derived from cocoons, has been employed as a biomedical material and is believed to be safe for human use. Silk peptide display various bioactivities, including anti-inflammatory, immune-regulatory, anti-tumor, anti-viral, and anti-bacterial. Although earlier investigations demonstrated that silk peptide stimulates macrophages and the production of pro-inflammatory cytokines, its effect on natural killer (NK) cell function has not yet been explored. In this study, we initially confirmed that silk peptide enhances NK cell activity in vitro and ex vivo. To assess the modulatory activity of silk peptide on NK cells, mice were fed various amounts of a silk peptide-supplemented diet for 2 months and the effects on immune stimulation, including NK cell activation, were evaluated. Oral administration of silk peptide significantly enhanced the proliferation of mitogen- or IL-2-stimulated splenocytes. In addition, oral silk peptide treatment enhanced the frequency and degree of maturation of NK cells in splenocytes. The same treatment also significantly enhanced the target cell cytolytic activity of NK cells, which was determined by cell surface CD107a expression and intracellular interferon-γ expression. Finally, oral administration of silk peptide stimulated T helper 1-type cytokine expression from splenic lymphocytes. Collectively, our results suggest that silk peptide potentiates NK cell activity in vivo and could be used as a compound for immune-modulating anti-tumor treatment.
참고문헌 (41)
- Mandal A, "Natural killer cells: in health and disease", Hematol Oncol Stem Cell Ther, 8, pp.47~55 (2015)[DOI]
- Moretta L, "Allorecognition by NK cells: nonself or no self", Immunol Today, 13, pp.300~306 (1992)[DOI]
- Vivier E, "Functions of natural killer cells", Nat Immunol, 9, pp.503~510 (2008)[DOI]
- Glas R, "Recruitment and activation of natural killer (NK) cells in vivo determined by the target cell phenotype. An adaptive component of NK cell-mediated responses", J Exp Med, 191, pp.129~138 (2000)[DOI]
- Smyth MJ, "NKT cells - conductors of tumor immunity?", Curr Opin Immunol, 14, pp.165~171 (2002)[DOI]
- Walzer T, "Natural-killer cells and dendritic cells: lunion fait la force", Blood, 106, pp.2252~2258 (2005)[DOI]
- Trinchieri G, "Biology of natural killer cells", Adv Immunol, 47, pp.187~376 (1989)
- Vivier E, "Targeting natural killer cells and natural killer T cells in cancer", Nat Rev Immunol, 12, pp.239~252 (2012)[DOI]
- Nagai N, "Enhancing effects of sericin on corneal wound healing in Otsuka Long-Evans Tokushima fatty rats as a model of human type 2 diabetes", Biol Pharm Bull, 32, pp.1594~1599 (2009)[DOI]
- 박동선, "Improving Effect of Silk Peptides on the Cognitive Function of Rats with Aging Brain Facilitated by D-Galactose", Biomolecules & Therapeutics, 19(2), pp.224~230 (2011), 한국응용약물학회
- Tae Kyun Kim, "Tyrosine-fortified Silk Amino Acids Improve Physical Function of Parkinson’s Disease Rats", Food Science and Biotechnology, 20(1), pp.79~84 (2011), 한국식품과학회
- Li P, "Amino acids and immune function", Br J Nutr, 98, pp.237~252 (2007)[DOI]
- Guadagni M, "Effects of inflammation and", or inactivity on the need for dietary protein, 2009, pp.12, 617~622
- Teramoto H, "Preparation of gel film from Bombyx mori silk sericin and its characterization as a wound dressing", Biosci Biotechnol Biochem, 72, pp.3189~3196 (2008)[DOI]
- Lee SH, "Silk and silkworm pupa peptides suppress adipogenesis in preadipocytes and fat accumulation in rats fed a high-fat diet", Eur J Nutr, 51, pp.1011~1019 (2012)[DOI]
- Seo CW, "Antihyperlipidemic and body fat-lowering effects of silk proteins with different fibroin", sericin compositions in mice fed with high fat diet, 2011, pp.59, 4192~4197
- Ahmad R, "Fibroin silk proteins from the nonmulberry silkworm Philosamia ricini are biochemically and immunochemically distinct from those of the mulberry silkworm Bombyx mori", DNA Cell Biol, 23, pp.149~154 (2004)[DOI]
- Jung EY, "Feeding silk protein hydrolysates to C57BL", KsJ-db, Nutr Res, pp.2010, 30[DOI]
- Gotoh K, "Sulfated fibroin, a novel sulfated peptide derived from silk, inhibits human immunodeficiency virus replication in vitro", Biosci Biotechnol Biochem, 64, pp.1664~1670 (2000)[DOI]
- Singh TM, "Role of fibrinopeptide B in early atherosclerotic lesion formation", Am J Surg, 160, pp.156~159 (1990)[DOI]
- Mei-sa Wang, "Silk fibroin peptide suppresses proliferation and induces apoptosis and cell cycle arrest in human lung cancer cells", Acta Pharmacologica Sinica, 40(4), pp.522~529 (2019), Springer Nature[DOI]
- Zhaorigetu S, "Supplemental silk protein, sericin, suppresses colon tumorigenesis in 1,2-dimethylhydrazine-treated mice by reducing oxidative stress and cell proliferation", Biosci Biotechnol Biochem, 65, pp.2181~2186 (2001)[DOI]
- Kaewkorn W, "Effects of silk sericin on the proliferation and apoptosis of colon cancer cells", Biol Res, 45, pp.45~50 (2012)[DOI]
- Uff CR, "Influence of soluble suture factors on in vitro macrophage function", Biomaterials, 16, pp.355~360 (1995)[DOI]
- Cui X, "A pilot study of macrophage responses to silk fibroin particles", J Biomed Mater Res A, 101, pp.1511~1517 (2013)
- Betts MR, "Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation", J Immunol Methods, 281, pp.65~78 (2003)[DOI]
- Aktas E, "Relationship between CD107a expression and cytotoxic activity", Cell Immunol, 254, pp.149~154 (2009)[DOI]
- Aubry JP, "Annexin V used for measuring apoptosis in the early events of cellular cytotoxicity", Cytometry, 37, pp.197~204 (1999)[DOI]
- Hayakawa Y, "Functional subsets of mouse natural killer cells", Immunol Rev, 214, pp.47~55 (2006)[DOI]
- Chiossone L, "Maturation of mouse NK cells is a 4-stage developmental program", Blood, 113, pp.5488~5496 (2009)[DOI]
- Artym J, "Orally administered lactoferrin restores humoral immune response in immunocompromised mice", Immunol Lett, 89, pp.9~15 (2003)[DOI]
- Kuhara T, "Oral administration of lactoferrin increases NK cell activity in mice via increased production of IL-18 and type I IFN in the small intestine", J Interferon Cytokine Res, 26, pp.489~499 (2006)[DOI]
- Egusa S, "Soybean protein fraction digested with neutral protease preparation, "Peptidase R", produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse", Int Immunopharmacol, 9, pp.931~936 (2009)[DOI]
- HeynKeyung Chang, "Lactoferrin Stimulates Mouse Macrophage to Express BAFF via Smad3 Pathway", Immune Network, 12(3), pp.84~88 (2012), 대한면역학회[DOI]
- 문정호, "Resistance to Toxoplasma gondii Infection in Mice Treated with Silk Protein by Enhanced Immune Responses", Parasites, Hosts and Diseases, 49(3), pp.303~308 (2011), 대한기생충학ㆍ열대의학회
- Igarashi K, "Blood pressure-depressing activity of a peptide derived from silkworm fibroin in spontaneously hypertensive rats", Biosci Biotechnol Biochem, 70, pp.517~520 (2006)[DOI]
- Ikegawa Y, "Amelioration of the progression of an atopic dermatitis-like skin lesion by silk peptide and identification of functional peptides", Biosci Biotechnol Biochem, 76, pp.473~477 (2012)[DOI]
- Gerberick GF, "Local lymph node assay: differentiating allergic and irritant responses using flow cytometry", Methods, 19, pp.48~55 (1999)[DOI]
- Sikorski EE, "Phenotypic analysis of lymphocyte subpopulations in lymph nodes draining the ear following exposure to contact allergens and irritants", Fundam Appl Toxicol, 34, pp.25~35 (1996)[DOI]
- 민보경, "Optimization of Large-Scale Expansion and Cryopreservation of Human Natural Killer Cells for Anti-Tumor Therapy", Immune Network, 18(4), pp.1~13 (2018), 대한면역학회[DOI]
- Wang R, "Natural killer cell-produced IFN-<TEX>$\gamma$<", TEX> and TNF-<TEX>${\alpha}$<, J Leukoc Biol, pp.2012, 91[DOI]