Radioprotective Effect of Silk Peptide Based on its Immunomodulatory Activities
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초록
The objective of this study was to investigate the radioprotective effect of silk peptide (SP)against gamma irradiation-induced damage via its immunomodulatory activity. The proliferation ofRAW 264.7 cells, peritoneal macrophage and splenocyte increased upon treatment with SP followedby irradiation in a dose-dependent manner. The TNF-α and IL-6 secretion levels were the highestin irradiated cells treated with 500 μg·ml-1 of SP. On the other hand, although the IFN-γ and IL-2levels were lower in the irradiated cells, the secretion of these cytokines increased when treated withSP. These results demonstrate that SP enhances the immune response by increasing the macrophageactivity in vitro. The splenocyte’s numbers decreased with gamma irradiation, but the administrationof SP (200 mg·kg-1 B.W.) significantly increased the splenocyte counts. Moreover, the SP treatmentsignificantly increased the IFN-γ and IL-2 secretion in irradiated mice. The mRNA expressionresults indicate that the SP administration prior to irradiation activated the Th1 cytokine (IFN-γ andIL-2) response as well as the expression of cytokines related to radioprotection (TNF-α, IL-6, andIL-1β). The results also showed that the SP administrated mice survived by 30% more than the PBSfeeding group when the mice were irradiated with a lethal dose (6.5 Gy). Thus, the overall resultsproposed that silk peptide could be a good candidate as radioprotective functional food.
Abstract
The objective of this study was to investigate the radioprotective effect of silk peptide (SP)against gamma irradiation-induced damage via its immunomodulatory activity. The proliferation ofRAW 264.7 cells, peritoneal macrophage and splenocyte increased upon treatment with SP followedby irradiation in a dose-dependent manner. The TNF-α and IL-6 secretion levels were the highestin irradiated cells treated with 500 μg·ml-1 of SP. On the other hand, although the IFN-γ and IL-2levels were lower in the irradiated cells, the secretion of these cytokines increased when treated withSP. These results demonstrate that SP enhances the immune response by increasing the macrophageactivity in vitro. The splenocyte’s numbers decreased with gamma irradiation, but the administrationof SP (200 mg·kg-1 B.W.) significantly increased the splenocyte counts. Moreover, the SP treatmentsignificantly increased the IFN-γ and IL-2 secretion in irradiated mice. The mRNA expressionresults indicate that the SP administration prior to irradiation activated the Th1 cytokine (IFN-γ andIL-2) response as well as the expression of cytokines related to radioprotection (TNF-α, IL-6, andIL-1β). The results also showed that the SP administrated mice survived by 30% more than the PBSfeeding group when the mice were irradiated with a lethal dose (6.5 Gy). Thus, the overall resultsproposed that silk peptide could be a good candidate as radioprotective functional food.
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