Dimerized, Not Monomeric, Translationally Controlled Tumor Protein Induces Basophil Activation and Mast Cell Degranulation in Chronic Urticaria
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Translationally controlled tumor protein (TCTP) is also known as histamine releasing factor as it has the ability to activate mast cells. To investigate the role of TCTP in the pathogenesis of chronic spontaneous urticaria (CSU), we evaluated serum level of TCTP and effect of TCTP on basophil and mast cell degranulation. TCTP levels in the sera from 116 CSU patients and 70 normal healthy controls (NCs) were measured by ELISA. CD203c expression on basophils from CSU patients and β-hexosaminidase release from Laboratory of Allergic Disease 2 mast cells were measured upon stimulation monomeric and dimeric TCTP. Non-reducing Western blot analysis was used for detecting dimeric TCTP. No difference was observed in serum TCTP levels between CSU patients and NCs (p=0.676). However, dimeric TCTP intensity on Western blot was stronger in CSU patients than in NCs. TCTP levels were higher in patients with severe CSU (p=0.049) and with IgG positivity to FcɛRIα (p=0.038). A significant positive correlation was observed between TCTP and eosinophil cationic protein levels (Spearman's rho=0.341; p=0.001). Both basophil and mast cell degranulation were significantly increased after stimulation with dimeric TCTP, but not with monomic TCTP. The ability of TCTP to activate basophil and mast cells is dependent on dimerization, suggesting that the inhibition of TCTP dimerization can be a therapeutic option for CSU. Association between TCTP levels and the presence of IgG to high affinity Fc epsilon receptor I alpha subunit in CSU patients indicates that autoimmune mechanisms may be involved in the dimerization of TCTP.
Abstract
Translationally controlled tumor protein (TCTP) is also known as histamine releasing factor as it has the ability to activate mast cells. To investigate the role of TCTP in the pathogenesis of chronic spontaneous urticaria (CSU), we evaluated serum level of TCTP and effect of TCTP on basophil and mast cell degranulation. TCTP levels in the sera from 116 CSU patients and 70 normal healthy controls (NCs) were measured by ELISA. CD203c expression on basophils from CSU patients and β-hexosaminidase release from Laboratory of Allergic Disease 2 mast cells were measured upon stimulation monomeric and dimeric TCTP. Non-reducing Western blot analysis was used for detecting dimeric TCTP. No difference was observed in serum TCTP levels between CSU patients and NCs (p=0.676). However, dimeric TCTP intensity on Western blot was stronger in CSU patients than in NCs. TCTP levels were higher in patients with severe CSU (p=0.049) and with IgG positivity to FcɛRIα (p=0.038). A significant positive correlation was observed between TCTP and eosinophil cationic protein levels (Spearman's rho=0.341; p=0.001). Both basophil and mast cell degranulation were significantly increased after stimulation with dimeric TCTP, but not with monomic TCTP. The ability of TCTP to activate basophil and mast cells is dependent on dimerization, suggesting that the inhibition of TCTP dimerization can be a therapeutic option for CSU. Association between TCTP levels and the presence of IgG to high affinity Fc epsilon receptor I alpha subunit in CSU patients indicates that autoimmune mechanisms may be involved in the dimerization of TCTP.
참고문헌 (35)
- Zuberbier T, "The EAACI", GA2LEN, WAO guideline for the definition, classification, diagnosis and management of urticaria, pp.Allergy, 2018[DOI]
- Sánchez-Borges M, "Diagnosis and treatment of urticaria and angioedema: a worldwide perspective", World Allergy Organ J, 5, pp.125~147 (2012)
- 이나미, "Epidemiology of Chronic Urticaria in Korea Using the Korean Health Insurance Database, 2010-2014", Allergy, Asthma & Immunology Research, 9(5), pp.438~445 (2017), 대한천식알레르기학회
- MacDonald SM, "Potential role of histamine releasing factor (HRF) as a therapeutic target for treating asthma and allergy", J Asthma Allergy, 5, pp.51~59 (2012)
- Xu A, "Expression of translationally controlled tumour protein is regulated by calcium at both the transcriptional and post-transcriptional level", Biochem J, 342, pp.683~689 (1999)
- Gachet Y, "The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle", J Cell Sci, 112, pp.1257~1271 (1999)
- MacDonald SM, "Studies of IgE-dependent histamine releasing factors: heterogeneity of IgE", J Immunol, 139, pp.506~512 (1987)
- Schroeder JT, "An immunoglobulin E-dependent recombinant histamine-releasing factor induces interleukin-4 secretion from human basophils", J Exp Med, 183, pp.1265~1270 (1996)
- Schroeder JT, "Recombinant histamine-releasing factor enhances IgE-dependent IL-4 and IL-13 secretion by human basophils", J Immunol, 159, pp.447~452 (1997)
- Vonakis BM, "Distinct characteristics of signal transduction events by histamine-releasing factor", translationally controlled tumor protein (HRF, Blood, pp.2008, 111[DOI]
- Kang HS, "Molecular identification of IgE-dependent histamine-releasing factor as a B cell growth factor", J Immunol, 166, pp.6545~6554 (2001)
- Vonakis BM, "Inhibition of cytokine gene transcription by the human recombinant histamine-releasing factor in human T lymphocytes", J Immunol, 171, pp.3742~3750 (2003)
- Kim M, "Dimerization of translationally controlled tumor protein is essential for its cytokine-like activity", PLoS One, 4, pp.e6464~ (2009)
- Toshiaki Kawakami, "Histamine-Releasing Factor and Immunoglobulins in Asthma and Allergy", Allergy, Asthma & Immunology Research, 6(1), pp.6~12 (2014), 대한천식알레르기학회
- Wantke F, "The human recombinant histamine releasing factor: functional evidence that it does not bind to the IgE molecule", J Allergy Clin Immunol, 103, pp.642~648 (1999)
- Huang X, "Synergistic actions of histamine-releasing factor and histamine releasing factor-reactive IgE in chronic urticaria", Int Arch Allergy Immunol, 172, pp.27~32 (2017)
- Jin XH, "Dimerized translationally controlled tumor protein-binding peptide ameliorates atopic dermatitis in NC", Nga mice, 2017, pp.18, 256~
- Ye YM, "Clinical evaluation of the computerized chronic urticaria-specific quality of life questionnaire in Korean patients with chronic urticaria", Clin Exp Dermatol, 37, pp.722~728 (2012)
- Ye YM, "Co-existence of chronic urticaria and metabolic syndrome: clinical implications", Acta Derm Venereol, 93, pp.156~160 (2013)
- Konstantinou GN, "EAACI", GA2LEN task force consensus report: the autologous serum skin test in urticaria, 2009, pp.64, 1256~1268
- Lee MF, "A rapid method of detecting autoantibody against FcεRIα for chronic spontaneous urticaria", PLoS One, 9, pp.e109565~ (2014)
- 예영민, "Increased Level of Basophil CD203c Expression Predicts Severe Chronic Urticaria", Journal of Korean Medical Science, 29(1), pp.43~47 (2014), 대한의학회
- Kuehn HS, "Measuring mast cell mediator release", Curr Protoc Immunol, Chapter 7, pp.Unit7.38~ (2010)
- Kashiwakura JC, "Histamine-releasing factor has a proinflammatory role in mouse models of asthma and allergy", J Clin Invest, 122, pp.218~228 (2012)
- Ferrer M, "Serum total tryptase levels are increased in patients with active chronic urticaria", Clin Exp Allergy, 40, pp.1760~1766 (2010)
- Siles R, "The utility of serum tryptase as a marker in chronic spontaneous urticaria", Acta Derm Venereol, 93, pp.354~355 (2013)
- Okayama Y, "Oxidative stress in allergic and inflammatory skin diseases", Curr Drug Targets Inflamm Allergy, 4, pp.517~519 (2005)
- Hosoki K, "Systems biology of free radicals and antioxidants", pp.3239~3266 (2014), Springer
- Doré KA, "Crystal structures of murine and human Histamine-Releasing Factor (HRF", TCTP) and a model for HRF dimerisation in mast cell activation, 2018, pp.93, 216~222
- Lee H, "Dimerized translationally controlled tumor protein increases interleukin-8 expression through MAPK and NF-κB pathways in a human bronchial epithelial cell line", Cell Biosci, 8, pp.13~ (2018)
- Gilfillan AM, "Integrated signalling pathways for mast-cell activation", Nat Rev Immunol, 6, pp.218~230 (2006)
- Lampinen M, "Cytokine-regulated accumulation of eosinophils in inflammatory disease", Allergy, 59, pp.793~805 (2004)
- Shakoory B, "The role of human mast cell-derived cytokines in eosinophil biology", J Interferon Cytokine Res, 24, pp.271~281 (2004)
- Lorenzo GD, "Blood eosinophils and serum eosinophil cationic protein in patients with acute and chronic urticaria", Mediators Inflamm, 5, pp.113~115 (1996)
- Tedeschi A, "Plasma levels and skin-eosinophil-expression of vascular endothelial growth factor in patients with chronic urticaria", Allergy, 64, pp.1616~1622 (2009)