본문 바로가기
KERD
논문KCI 등재

Basophil Markers for Identification and Activation in the Indirect Basophil Activation Test by Flow Cytometry for Diagnosis of Autoimmune Urticaria

김종근|최봉석|원동일|Zehwan Kim
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2016.01|Annals of Laboratory Medicine|제36권 제1호|pp. 28-35

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

Flow cytometryChronic urticariaDonorBasophilMarkersAutoimmune urticaria
주제분류병리학

초록

Background: The indirect basophil activation test using flow cytometry is a promising tool for autoimmune urticaria diagnosis. We aimed to identify better donor basophils (from atopic vs. non-atopic donors and interleukin-3 primed vs. unprimed basophils) and improve basophil identification and activation markers (eotaxin CC chemokine receptor-3 [CCR3] vs. CD123 and CD63 vs. CD203c). Methods: Donor basophils were obtained from non-atopic and atopic group O donors. Positive control sera were artificially prepared to simulate autoimmune urticaria patients’ sera. Patient sera were obtained from nine children with chronic urticaria. Assay sensitivity was compared among each variation by using positive control sera (n=21), applying cutoff values defined from negative control sera (n=20). Results: For basophil identification, a combination of CCR3 and CD123 markers revealed a higher correlation with automated complete blood count (r=0.530) compared with that observed using CD123 (r=0.498) or CCR3 alone (r=0.195). Three activation markers on the atopic donor basophils attained 100% assay sensitivity: CD203c on unprimed basophils, CD63+CD203+ or CD63 alone on primed basophils; however, these markers on the non-atopic donor basophils attained lower assay sensitivity. Conclusions: For basophil identification markers, a combination of CD123 and CCR3 is recommended, while CD123 alone may be used as an alternative. Donor basophils should be obtained from an atopic donor. For basophil activation markers, either CD203c alone on unprimed basophils or CD203c and CD63 on primed basophils are recommended, while CD63 alone on primed basophils may be used as an alternative.

Abstract

Background: The indirect basophil activation test using flow cytometry is a promising tool for autoimmune urticaria diagnosis. We aimed to identify better donor basophils (from atopic vs. non-atopic donors and interleukin-3 primed vs. unprimed basophils) and improve basophil identification and activation markers (eotaxin CC chemokine receptor-3 [CCR3] vs. CD123 and CD63 vs. CD203c). Methods: Donor basophils were obtained from non-atopic and atopic group O donors. Positive control sera were artificially prepared to simulate autoimmune urticaria patients’ sera. Patient sera were obtained from nine children with chronic urticaria. Assay sensitivity was compared among each variation by using positive control sera (n=21), applying cutoff values defined from negative control sera (n=20). Results: For basophil identification, a combination of CCR3 and CD123 markers revealed a higher correlation with automated complete blood count (r=0.530) compared with that observed using CD123 (r=0.498) or CCR3 alone (r=0.195). Three activation markers on the atopic donor basophils attained 100% assay sensitivity: CD203c on unprimed basophils, CD63+CD203+ or CD63 alone on primed basophils; however, these markers on the non-atopic donor basophils attained lower assay sensitivity. Conclusions: For basophil identification markers, a combination of CD123 and CCR3 is recommended, while CD123 alone may be used as an alternative. Donor basophils should be obtained from an atopic donor. For basophil activation markers, either CD203c alone on unprimed basophils or CD203c and CD63 on primed basophils are recommended, while CD63 alone on primed basophils may be used as an alternative.

참고문헌 (26)

  1. Marrouche N, "Childhood urticaria", Curr Opin Allergy Clin Immunol, 12, pp.485~490 (2012)
  2. Fiebiger E, "Anti-FcepsilonRIalpha autoantibodies in autoimmune-mediated disorders. Identification of a structure-function relationship", J Clin Invest, 101, pp.243~251 (1998)
  3. Irinyi B, "Clinical and laboratory examinations in the subgroups of chronic urticaria", Int Arch Allergy Immunol, 144, pp.217~225 (2007)
  4. Ebo DG, "Basophil activation test by flow cytometry: present and future applications in allergology", Cytometry B Clin Cytom, 74, pp.201~210 (2008)
  5. Marone G, "The clinical relevance of basophil releasability", J Allergy Clin Immunol, 94, pp.1293~1303 (1994)
  6. Zuberbier T, "Histamine releasability of basophils and skin mast cells in chronic urticaria", Allergy, 51, pp.24~28 (1996)
  7. Szegedi A, "Significant correlation between the CD63 assay and the histamine release assay in chronic urticaria", Br J Dermatol, 155, pp.67~75 (2006)
  8. Sabroe RA, "The autologous serum skin test: a screening test for autoantibodies in chronic idiopathic urticaria", Br J Dermatol, 140, pp.446~452 (1999)
  9. Frezzolini A, "Serum-induced basophil CD63 expression by means of a tricolour flow cytometric method for the in vitro diagnosis of chronic urticaria", Allergy, 61, pp.1071~1077 (2006)
  10. Gyimesi E, "Basophil CD63 expression assay on highly sensitized atopic donor leucocytes-a useful method in chronic autoimmune urticaria", Br J Dermatol, 151, pp.388~396 (2004)
  11. Ocmant A, "Flow cytometry for basophil activation markers: the measurement of CD203c up-regulation is as reliable as CD63 expression in the diagnosis of cat allergy", J Immunol Methods, 320, pp.40~48 (2007)
  12. Cozon G, "Detection of activated basophils using flow cytometry for diagnosis in atopic patients", Allergol Immunopathol, 27, pp.182~187 (1999)
  13. Ebo DG, "Comments regarding ‘Marked improvement of the basophil activation test by detecting CD203c instead of CD63’ by Boumiza et al", Clin Exp Allergy, 33, pp.849~ (2003)
  14. Gentinetta T, "Individual IL-3 priming is crucial for consistent in vitro activation of donor basophils in patients with chronic urticaria", J Allergy Clin Immunol, 128, pp.1227~1234 (2011)
  15. Marone G, "Human basophil releasability. II. Changes in basophil releasability in patients with atopic dermatitis", J Invest Dermatol, 87, pp.19~23 (1986)
  16. Sturm EM, "CD203c-based basophil activation test in allergy diagnosis: characteristics and differences to CD63 upregulation", Cytometry B Clin Cytom, 78, pp.308~318 (2010)
  17. Wedi B, "Chronic urticaria serum induces histamine release, leukotriene production, and basophil CD63 surface expression--inhibitory effects of anti-inflammatory drugs", J Allergy Clin Immunol, 105, pp.552~560 (2000)
  18. Chirumbolo S, "The use of IL-3 in basophil activation tests is the real pitfall", Cytometry B Clin Cytom, 80, pp.137~138 (2011)
  19. Hausmann OV, "Robust expression of CCR3 as a single basophil selection marker in flow cytometry", Allergy, 66, pp.85~91 (2011)
  20. Ducrest S, "Flowcytometric analysis of basophil counts in human blood and inaccuracy of hematology analyzers", Allergy, 60, pp.1446~1450 (2005)
  21. Chirumbolo S, "CCR3 as a single selection marker compared to CD123", HLADR to isolate basophils in flow cytometry: some comments, 2011, pp.79, 102~106
  22. Ferrer M, "Secretion of cytokines, histamine and leukotrienes in chronic urticaria", Int Arch Allergy Immunol, 129, pp.254~260 (2002)
  23. Hennersdorf F, "Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation", Cell Res, 15, pp.325~335 (2005)
  24. Yasnowsky KM, "Chronic urticaria sera increase basophil CD203c expression", J Allergy Clin Immunol, 117, pp.1430~1434 (2006)
  25. Laidlaw TM, "Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcɛRI", J Allergy Clin Immunol, 127, pp.815~822 (2011)
  26. Rashid A, "Review: diagnostic and therapeutic applications of rat basophilic leukemia cells", Mol Immunol, 52, pp.224~228 (2012)

관련 논문AI추천

Basophil Activation Test for Chronic UrticariaSalvatore Chirumbolo대한진단검사의학회 · Annals of Laboratory Medicine2016 · 제36권 제5호Correlations between Anti-Mast Cell Autoantibodies and Chronic Idiopathic UrticariaChunyu Han, Jian Yang, Jie Li, Kang Erxun, Liwei Sun대한피부과학회 · Annals of Dermatology2014 · 제26권 제2호The Relation of Autologous Serum Skin Test and Autologous Plasma Skin Test Result with Various Clinical and Laboratory Findings in Patients with Chronic Spontaneous Urticaria김선미, 박경훈, 배유인, 최정희대한피부과학회 · Annals of Dermatology2020 · 제32권 제4호Biomarkers for Short-Term Omalizumab Response in Chronic Spontaneous Urticaria김락균, 김수민, 김완진, 류수락, 박경희, 박정원, 박창욱, 박희웅, 오종욱, 이재현, 이지원, 조한경대한피부과학회 · Annals of Dermatology2024 · 제36권 제6호Dimerized, Not Monomeric, Translationally Controlled Tumor Protein Induces Basophil Activation and Mast Cell Degranulation in Chronic Urticaria박해심, 양은미, 예영민, 이경림, 이희원, Bastsetseg Ulambayar대한면역학회 · Immune Network2019 · 제19권 제3호Soluble CD93 in Serum as a Marker of Allergic Inflammation박경희, 박윤희, 박중원, 박혜정, 손영웅, 심다운, 이상철, 이재현, 정경용, 한희재연세대학교의과대학 · Yonsei Medical Journal2017 · 제58권 제3호

이 간행물의 다른 논문 (16건)

Immature Platelet Fraction in Septic Patients: Clinical Relevance of Immature Platelet Fraction is Limited to the Sensitive and Accurate Discrimination of Septic Patients From Non-Septic Patients, Not to the Discrimination of Sepsis Severity하상욱, 조영욱, 홍상범, 박찬정, 장성수, 박상혁pp. 1-8Epidemiological Study of Erythromycin-Resistant Streptococcus pyogenes From Korea and Japan by emm Genotyping and Multilocus Sequence TypingMitsuo Kaku, 김선주, Kazuaki Arai, 이동현, 고은하, Takashi Takahashi, Haruno Yoshida, Hisakazu Yanopp. 9-14Rapid Detection of Pseudomonas aeruginosa and Acinetobacter baumannii Harboring blaVIM-2, blaIMP-1 and blaOXA-23 Genes by Using Loop-Mediated Isothermal Amplification Methods김형선, 김혜진, 이재면, 용동은, 윤상선pp. 15-22Comparison of Automated Treponemal and Nontreponemal Test Algorithms as First-Line Syphilis Screening Assays박성연, 채석래, 허희진, 정재우pp. 23-27Frequency and Pattern of Noninfectious Adverse Transfusion Reactions at a Tertiary Care Hospital in Korea김현옥, 김신영, Essam Alghamdi, 조주영, 최승준pp. 36-41
원문 보기