Royal jelly enhances migration of human dermal fibroblasts and alters the levels of cholesterol and sphinganine in an in vitro wound healing model
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Oral administration of royal jelly (RJ) promotes wound healing in diabetic mice. Concerns have arisen regarding the efficacy of RJ on the wound healing process of normal skin cells. In this study, a wound was created by scratching normal human dermal fibroblasts, one of the major cells involved in the wound healing process. The area was promptly treated with RJ at varying concentrations of 0.1, 1.0, or 5 mg/ml for up to 48hrs and migration was analyzed by evaluating closure of the wound margins. Furthermore, altered levels of lipids, which were recently reported to participate in the wound healing process, were analyzed by HPTLC and HPLC. Migration of fibroblasts peaked at 24 hrs after wounding. RJ treatment significantly accelerated the migration of fibroblasts in a dose-dependent manner at 8 hrs. Although RJ also accelerated the migration of fibroblasts at both 20 hrs and 24 hrs after wounding, the efficacy was less potent than at 8 hrs. Among various lipid classes within fibroblasts,the level of cholesterol was significantly decreased at 8 hrs following administration of both 0.1 ug/ml and 5 mg/ml RJ. Despite a dose-dependent increase in sphinganines, the levels of sphingosines, ceramides, and glucosylceramides were not altered with any concentration of RJ. We demonstrated that RJ enhances the migration of fibroblasts and alters the levels of various lipids involved in the wound healing process.
Abstract
Oral administration of royal jelly (RJ) promotes wound healing in diabetic mice. Concerns have arisen regarding the efficacy of RJ on the wound healing process of normal skin cells. In this study, a wound was created by scratching normal human dermal fibroblasts, one of the major cells involved in the wound healing process. The area was promptly treated with RJ at varying concentrations of 0.1, 1.0, or 5 mg/ml for up to 48hrs and migration was analyzed by evaluating closure of the wound margins. Furthermore, altered levels of lipids, which were recently reported to participate in the wound healing process, were analyzed by HPTLC and HPLC. Migration of fibroblasts peaked at 24 hrs after wounding. RJ treatment significantly accelerated the migration of fibroblasts in a dose-dependent manner at 8 hrs. Although RJ also accelerated the migration of fibroblasts at both 20 hrs and 24 hrs after wounding, the efficacy was less potent than at 8 hrs. Among various lipid classes within fibroblasts,the level of cholesterol was significantly decreased at 8 hrs following administration of both 0.1 ug/ml and 5 mg/ml RJ. Despite a dose-dependent increase in sphinganines, the levels of sphingosines, ceramides, and glucosylceramides were not altered with any concentration of RJ. We demonstrated that RJ enhances the migration of fibroblasts and alters the levels of various lipids involved in the wound healing process.
참고문헌 (52)
- Viuda-Martos M, "Functional properties of honey, propolis, and royal jelly", J Food Sci, 73, pp.R117~R124 (2008)
- Fujii A, "Augmentation of wound healing by royal jelly (RJ) in streptozotocin-diabetic rats", Jpn J Pharmacol, 53, pp.331~337 (1990)
- Werner S, "Regulation of wound healing by growth factors and cytokines", Physiol Rev, 83, pp.835~870 (2003)
- Guo H, "Royal jelly supplementation improves lipoprotein metabolism in humans", J Nutr Sci Vitaminol, 53, pp.345~348 (2007)
- Molan PC, "The role of honey in the management of wounds", J Wound Care, 8, pp.415~418 (1999)
- Salazar-Olivo LA, "Screening of biological activities present in honeybee (Apis mellifera) royal jelly", Toxicol In Vitro, 19, pp.645~651 (2005)
- Karsten Münstedt, "Royal Jelly Reduces the Serum Glucose Levels in Healthy Subjects", Journal of Medicinal Food, 12(5), pp.1170~1172 (2009), 한국식품영양과학회
- Gasic S, "Evaluation of the immunomodulatory activities of royal jelly components in vitro", Immunopharmacol Immunotoxicol, 29, pp.521~536 (2007)
- Clark RA, "Regulation of fibroplasia in cutaneous wound repair", Am J Med Sci, 306, pp.42~48 (1993)
- Vicanova J, "Epidermal lipid metabolism of cultured skin substitutes during healing of full-thickness wounds in athymic mice", Wound Repair Regen, 5, pp.329~338 (1997)
- Altavilla D, "Inhibition of lipid peroxidation restores impaired vascular endothelial growth factor expression and stimulates wound healing and angiogenesis in the genetically diabetic mouse", Diabetes, 50, pp.667~674 (2001)
- Proksch E, "Regulation of the epidermal permeability barrier by lipids and hyperproliferation", Hautarzt, 43, pp.331~338 (1992)
- Taniguchi Y, "Oral administration of royal jelly inhibits the development of atopic dermatitis-like skin lesions in NC", Nga mice, 2003, pp.3, 1313~1324
- Bincoletto C, "Effects produced by royal jelly on haematopoiesis: Relation with host resistance against ehrlich ascites tumour challenge", Int Immunopharmacol, 5, pp.679~688 (2005)
- Witte MB, "General proniples of wound healing", Surg Clin North Am, 77, pp.509~528 (1997)
- Berg EL, "Chemical target and pathway toxicity mechanisms defined in primary human cell systems", J Pharmacol Toxicol Methods, 61, pp.3~15 (2010)
- Denizot F, "Rapid colorimetric assay for cell growth and survival: Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability", J Immunol Methods, 89, pp.271~277 (1986)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
- Lo CM, "Nonmuscle myosin IIb is involved in the guidance of fibroblast migration", Mol Biol Cell, 15, pp.982~989 (2004)
- Bligh EG, "A rapid method of total lipid extraction and purification", Can J Biochem Physiol, 37, pp.911~917 (1959)
- Melnik BC, "Microanalytical screening of all major stratum corneum lipids by sequential high-performance thin-layer chromatography", J Invest Dermatol, 92, pp.231~234 (1989)
- Folch J, "A simple method for the isolation and purification of total lipids from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
- Patella V, "Protein fv produced during vital hepatitis is a novel activator of human basophiles and mast cells", J Immunol, 151, pp.5685~5698 (1993)
- Martin P, "Wound healing--aiming for perfect skin regeneration", Science, 276, pp.75~81 (1997)
- Singer AJ, "Cutaneous wound healing", N Engl J Med, 341, pp.738~746 (1999)
- Matuszewska B, "Acidic fibroblast growth factor: Evaluation of topical formulations in a diabetic mouse wound healing model", Pharm Res, 11, pp.65~71 (1994)
- Turkmen Z, "Protective role of royal jelly (honeybee) on genotoxicity and lipid peroxidation, induced by petroleum wastewater, in Allium cepa L. root tips", Environ Technol, 30, pp.1205~1214 (2009)
- Holleran WM, "Regulation of epidermal sphingolipid synthesis by permeability barrier function", J Lipid Res, 32, pp.1151~1158 (1991)
- Rother J, "Biosynthesis of sphingolipids: Dihydroceramide and not sphinganine is desaturated by cultured cells", Biochem Biophys Res Commun, 189, pp.14~20 (1992)
- Hakomori S, "Bifunctional role of glycosphingolipids. modulators for transmembrane signaling and mediators for cellular interactions", J Biol Chem, 265, pp.18713~18716 (1990)
- Hannun YA, "Functions of sphingolipids and sphingolipid breakdown products in cellular regulation", Science, 243, pp.500~507 (1989)
- Spiegel S, "Sphingolipid metabolism and cell growth regulation", FASEB J, 10, pp.1388~1397 (1996)
- Huwiler A, "Physiology and pathophysiology of sphingolipid metabolism and signaling", Biochim Biophys Acta, 1485, pp.63~99 (2000)
- Radin NS, "Apoptotic death by ceramide: Will the real killer please stand up?", Med Hypotheses, 57, pp.96~100 (2001)
- Goswami R, "Does ceramide play a role in neural cell apoptosis?", J Neurosci Res, 60, pp.141~149 (2000)
- Tan NS, "Peroxisome proliferatoractivated receptor (PPAR)-beta as a target for wound healing drugs: What is possible?", Am J Clin Dermatol, 4, pp.523~530 (2003)
- Candela M, "Sphingosine synergistically stimulates tumor necrosis factor alpha-induced prostaglandin E2 production in human fibroblasts", J Exp Med, 174, pp.1363~1369 (1991)
- Liao J, "Chemistry and biology of sphingolipids", Tetrahedron, 61, pp.4715~4733 (2005)
- Hannun YA, "Enzymes of sphingolipid metabolism: From modular to integrative signaling", Biochemistry, 40, pp.4893~4903 (2001)
- Brown LF, "Fibroblast migration in fibrin gel matrices", Am J Pathol, 142, pp.273~283 (1993)
- Takahama H, "Food-induced anaphylaxis caused by ingestion of royal jelly", J Dermatol, 33, pp.424~426 (2006)
- Yang XY, "10-hydroxy-2-decenoic acid from royal jelly: A potential medicine for RA", J Ethnopharmacol, 128, pp.314~321 (2010)
- Izuta H, "10-hydroxy-2-decenoic acid, a major fatty acid from royal jelly, inhibits VEGF-induced angiogenesis in human umbilical vein endothelial cells", Evid Based Complement Alternat Med, 6, pp.489~494 (2009)
- Fujiwara S, "A potent antibacterial protein in royal jelly. purification and determination of the primary structure of royalisin", J Biol Chem, 265, pp.11333~11337 (1990)
- Kimura M, "350-kDa royal jelly glycoprotein (apisin), which stimulates proliferation of human monocytes, bears the beta1-3galactosylated N-glycan: Analysis of the N-glycosylation site", Biosci Biotechnol Biochem, 67, pp.2055~2058 (2003)
- Koya-Miyata S, "Identification of a collagen production-promoting factor from an extract of royal jelly and its possible mechanism", Biosci Biotechnol Biochem, 68, pp.767~773 (2004)
- Blum MS, "10-hydroxy-delta 2-decanoic acid, an antibiotic found in royal jelly", Science, 130, pp.452~453 (1959)
- Townsend GF, "Activity of 10-hydroxydecanoic acid from royal jelly against experimental leukaemia and ascitic tumours", Nature, 183, pp.1270~1271 (1959)
- Varga J, "Stimulation of normal human fibroblast collagen production and processing by transforming growth factor-β", Biochem Biophys Res Commun, 138, pp.974~980 (1986)
- Echigo T, "Comparative studies on chemical composition of honey, royal jelly and pollen loads", Bull Fac Agr Tamagawa Univ, 26, pp.1~26 (1986)
- Choi SW, "The wound-healing effect of a glycoprotein fraction isolated from aloe vera", Br J Dermatol, 145, pp.535~545 (2001)
- Sano O, "Characterization of royal jelly proteins in both Africanized and European honeybees (Apis mellifera) by two-dimensional gel electrophoresis", J Agric Food Chem, 52, pp.15~20 (2004)
