Quercetin ameliorates hyperglycemia and dyslipidemia and improves antioxidant status in type 2 diabetic db/db mice
2
조회
0
다운로드
11
인용
0
참고문헌
키워드
초록
This study investigated the hypoglycemic, hypolipidemic, and antioxidant effects of dietary quercetin in an animal model of type 2 diabetes mellitus. Four-week-old C57BL/KsJ-db/db mice (n = 18) were offered an AIN-93G diet or a diet containing quercetin at 0.04% (low quercetin, LQE) or 0.08% of the diet (high quercetin, HQE) for 6 weeks after 1 week of adaptation. Plasma glucose, insulin, adiponectin, and lipid profiles, and lipid peroxidation of the liver were determined. Plasma glucose levels were significantly lower in the LQE group than in the control group, and those in the HQE group were even further reduced compared with the LQE group. The homeostasis model assessment for insulin resistance (HOMA-IR)showed lower values for LQE and HQE than for the control group without significant influence on insulin levels. High quercetin increased plasma adiponectin compared with the control group. Plasma triglycerides in the LQE and HQE groups were lower than those in the control group. Supplementation with high quercetin decreased plasma total cholesterol and increased HDL-cholesterol compared with the control group. Consumption of low and high quercetin reduced thiobarbituric acid reactive substances (TBARS) levels and elevated activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver. Thus, quercetin could be effective in improving hyperglycemia, dyslipidemia, and antioxidant status in type 2 diabetes.
Abstract
This study investigated the hypoglycemic, hypolipidemic, and antioxidant effects of dietary quercetin in an animal model of type 2 diabetes mellitus. Four-week-old C57BL/KsJ-db/db mice (n = 18) were offered an AIN-93G diet or a diet containing quercetin at 0.04% (low quercetin, LQE) or 0.08% of the diet (high quercetin, HQE) for 6 weeks after 1 week of adaptation. Plasma glucose, insulin, adiponectin, and lipid profiles, and lipid peroxidation of the liver were determined. Plasma glucose levels were significantly lower in the LQE group than in the control group, and those in the HQE group were even further reduced compared with the LQE group. The homeostasis model assessment for insulin resistance (HOMA-IR)showed lower values for LQE and HQE than for the control group without significant influence on insulin levels. High quercetin increased plasma adiponectin compared with the control group. Plasma triglycerides in the LQE and HQE groups were lower than those in the control group. Supplementation with high quercetin decreased plasma total cholesterol and increased HDL-cholesterol compared with the control group. Consumption of low and high quercetin reduced thiobarbituric acid reactive substances (TBARS) levels and elevated activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver. Thus, quercetin could be effective in improving hyperglycemia, dyslipidemia, and antioxidant status in type 2 diabetes.
참고문헌 (41)
- Cheng D, "Prevalence, predisposition and prevention of type II diabetes", Nutr Metab (Lond), 2, pp.29~ (2005)
- King H, "Global burden of diabetes,1995-2025: prevalence, numerical estimates, and projections", Diabetes Care, 21, pp.1414~1431 (1998)
- O'Keefe JH Jr, "Improving the adverse cardiovascular prognosis of type 2 diabetes", Mayo Clin Proc, 74, pp.171~180 (1999)
- Maritim AC, "Diabetes, oxidative stress, and antioxidants: a review", J Biochem Mol Toxicol, 17, pp.24~38 (2003)
- Rahimi R, "A review on the role of antioxidants in the management of diabetes and its complications", Biomed Pharmacother, 59, pp.365~373 (2005)
- Garg A, "Management of dyslipidemia in NIDDM", Diabetes Care, 13, pp.153~169 (1990)
- American Diabetes Association, "Management of dyslipidemia in adults with diabetes", Diabetes Care, 26(Suppl 1), pp.583~586 (2003)
- Giacco F, "Oxidative stress and diabetic complications", Circ Res, 107, pp.1058~1070 (2010)
- Rao YK, "Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: a comparative study", Food Chem Toxicol, 45, pp.1770~1776 (2007)
- Boots AW, "Health effects of quercetin: from antioxidant to nutraceutical", Eur J Pharmacol, 585, pp.325~337 (2008)
- Mahesh T, "Quercetin allievates oxidative stress in streptozotocin-induced diabetic rats", Phytother Res, 18, pp.123~127 (2004)
- Dias AS, "Quercetin decreases oxidative stress, NFkappaB activation, and iNOS overexpression in liver of streptozotocin- induced diabetic rats", J Nutr, 135, pp.2299~2304 (2005)
- Kobori M, "Chronic dietary intake of quercetin alleviates hepatic fat accumulation associated with consumption of a Western-style diet in C57", BL6J mice, 2011, pp.55, 530~540
- Vessal M, "Antidiabetic effects of quercetin in streptozocin-induced diabetic rats", Comp Biochem Physiol C Toxicol Pharmacol, 135, pp.357~364 (2003)
- Torres-Piedra M, "A comparative study of flavonoid analogues on streptozotocin-nicotinamide induced diabetic rats: quercetin as a potential antidiabetic agent acting via 11beta-hydroxysteroid dehydrogenase type 1 inhibition", Eur J Med Chem, 45, pp.2606~2612 (2010)
- Ishikawa A, "Characterization of inhibitors of postprandial hyperglycemia from the leaves of Nerium indicum", J Nutr Sci Vitaminol (Tokyo), 53, pp.166~173 (2007)
- Jo SH, "Comparison of antioxidant potential and rat intestinal α-glucosidases inhibitory activities of quercetin, rutin, and isoquercetin", Int J Applied Res Nat Prod, 2, pp.52~60 (2009)
- 김지혜, "Quercetin attenuates fasting and postprandial hyperglycemia in animal models of diabetes mellitus", Nutrition Research and Practice, 5(2), pp.107~111 (2011), 한국영양학회
- Ramachandra R, "Quercetin alleviates activities of intestinal and renal disaccharidases in streptozotocininduced diabetic rats", Mol Nutr Food Res, 49, pp.355~360 (2005)
- Shetty AK, "Antidiabetic influence of quercetin in streptozotocin-induced diabetic rats", Nutr Res, 24, pp.373~381 (2004)
- Reeves PG, "AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet", J Nutr, 123, pp.1939~1951 (1993)
- Haffner SM, "The homeostasis model in the San Antonio Heart Study", Diabetes Care, 20, pp.1087~1092 (1997)
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.351~358 (1979)
- Aebi H, "Catalase, In Methods of Enzymatic Analysis", pp.673~683 (1974), Academic Press
- Paglia DE, "Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase", J Lab Clin Med, 70, pp.158~169 (1967)
- Marklund S, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.469~474 (1974)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
- Standl E, "Effect of acarbose on additional insulin therapy in type 2 diabetic patients with late failure of sulphonylurea therapy", Diabetes Obes Metab, 1, pp.215~220 (1999)
- 이시훈, "Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage", AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 294(1), pp.15~26, AMER PHYSIOLOGICAL SOC
- Sheng T, "Adiponectin and its association with insulin resistance and type 2 diabetes", J Genet Genomics, 35, pp.321~326 (2008)
- The Diabetes Control and Complications Trial Research Group, "The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus", N Engl J Med, 329, pp.977~986 (1993)
- Gnoni GV, "Quercetin inhibits fatty acid and triacylglycerol synthesis in rat-liver cells", Eur J Clin Invest, 39, pp.761~768 (2009)
- Azuma K, "Acarbose, an alpha-glucosidase inhibitor, improves endothelial dysfunction in Goto-Kakizaki rats exhibiting repetitive blood glucose fluctuation", Biochem Biophys Res Commun, 345, pp.688~693 (2006)
- Haffner SM, "Management of dyslipidemia in adults with diabetes", Diabetes Care, 21, pp.160~178 (1998)
- Stamler J, "Diabetes, other risk factors, and 12-yr cardiovascular mortality for men screened in the Multiple Risk Factor Intervention Trial", Diabetes Care, 16, pp.434~444 (1993)
- Witztum JL, "The oxidative modification hypothesis of atherosclerosis: does it hold for humans?", Trends Cardiovasc Med, 11, pp.93~102 (2001)
- Du X, "Insulin resistance reduces arterial prostacyclin synthase and eNOS activities by increasing endothelial fatty acid oxidation", J Clin Invest, 116, pp.1071~1080 (2006)
- Hanasaki Y, "The correlation between active oxygens scavenging and antioxidative effects of flavonoids", Free Radic Biol Med, 16, pp.845~850 (1994)
- Galati G, "Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties", Free Radic Biol Med, 37, pp.287~303 (2004)
- Harman D, "The aging process: major risk factor for disease and death", Proc Natl Acad Sci U S A, 88, pp.5360~5363 (1991)
- Dröge W, "Free radicals in the physiological control of cell function", Physiol Rev, 82, pp.47~95 (2002)