Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus
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Hyperglycemia in the diabetic state increases oxidative stress and antioxidant therapy can be strongly correlated with decreased risks for diabetic complications. The purpose of this study is to determine antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes. The antioxidant activity of garlic and aged black garlic was measured as the activity in scavenging free radicals by the trolox equivalent antioxidant capacity (TEAC) assay. Three week-old db/db mice were fed AIN-93G diet or diet containing 5% freeze-dried garlic or aged black garlic for 7 weeks after 1 week of adaptation. Hepatic levels of lipid peroxides and activities of antioxidant enzymes were measured. TEAC values of garlic and aged black garlic were 13.3 ± 0.5 and 59.2 ± 0.8 μmol/g wet weight, respectively. Consumption of aged black garlic significantly decreased hepatic thiobarbituric acid reactive substances (TBARS) level compared with the garlic group which showed lower TBARS level than control group (p<0.05). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of garlic and aged black garlic group were significantly elevated compared to the control group. Catalase (CAT) activity of aged black garlic group was increased compared with the control group. These results show that aged black garlic exerts stronger antioxidant activity than garlic in vitro and in vivo, suggesting garlic and aged black garlic, to a greater extent, could be useful in preventing diabetic complications.
Abstract
Hyperglycemia in the diabetic state increases oxidative stress and antioxidant therapy can be strongly correlated with decreased risks for diabetic complications. The purpose of this study is to determine antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes. The antioxidant activity of garlic and aged black garlic was measured as the activity in scavenging free radicals by the trolox equivalent antioxidant capacity (TEAC) assay. Three week-old db/db mice were fed AIN-93G diet or diet containing 5% freeze-dried garlic or aged black garlic for 7 weeks after 1 week of adaptation. Hepatic levels of lipid peroxides and activities of antioxidant enzymes were measured. TEAC values of garlic and aged black garlic were 13.3 ± 0.5 and 59.2 ± 0.8 μmol/g wet weight, respectively. Consumption of aged black garlic significantly decreased hepatic thiobarbituric acid reactive substances (TBARS) level compared with the garlic group which showed lower TBARS level than control group (p<0.05). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of garlic and aged black garlic group were significantly elevated compared to the control group. Catalase (CAT) activity of aged black garlic group was increased compared with the control group. These results show that aged black garlic exerts stronger antioxidant activity than garlic in vitro and in vivo, suggesting garlic and aged black garlic, to a greater extent, could be useful in preventing diabetic complications.
참고문헌 (40)
- Abei H, "Abei H (1974). Catalase in the Method of Enzymatic Analysis vol.2", pp.673~684 (1974), Academic Press
- Ahmad MS, "Aged garlic extract and S-allyl cysteine prevent formation of advanced glycation endproducts", Eur J Pharmacol, 561, pp.32~38 (2007)
- Al-Qattan K, "Garlic (Allium sativum) and ginger (Zingiber officinale) attenuate structural nephropathy progression in streptozotocin-induced diabetic rats", The European e-Journal of Clinical Nutrition and Metabolism, 3, pp.e62~e71 (2008)
- Amagase H, "Clarifying the real bioactive constituents of garlic", J Nutr, 136, pp.716S~725S (2006)
- Amagase H, "Intake of garlic and its bioactive components", J Nutr, 131, pp.955S~962S (2001)
- American Diabetes Association, "Management of dyslipidemia in adults with diabetes (Position Statement)", Diabetes Care, 22, pp.56~59 (1999)
- Banerjee SK, "Garlic as an antioxidant: the good, the bad and the ugly", Phytother Res, 17, pp.97~106 (2003)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
- "Diabetes Surveillance Report, Atlanta, GA: US Department of Health and Human Services"[URL]
- Ceriello A, "Oxidative stress and diabetes-associated complications", Endocr Pract, 12, pp.60~62 (2006)
- Cheng AYY, "Intestinal absorption inhibitors for type 2 diabetes mellitus: prevention and treatment", Drug Discovery Today: Therapeutic Strategies, 1, pp.201~206 (2004)
- Corzo-Martinez M, "Biological properties of onions and garlic", Trends in Food Science & Technology, 18, pp.609~625 (2007)
- Drobiova H, "Garlic increases antioxidant levels in diabetic and hypertensive rats determined by a modified peroxidase Method", Evid Based Complement Alternat Med eCAM, pp.1~7 (2009)
- Eidi A, "Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin-induced diabetic rats", Phytomedicine, 13, pp.624~629 (2006)
- Fujita H, "Long-term ingestion of a fermented soybean-derived Touchi-extract with alphaglucosidase inhibitory activity is safe and effective in humans with borderline and mild type-2 diabetes", J Nutr, 131, pp.2105~2108 (2001)
- Haron D, "The aging: major risk factor for disease and death", Proc Natl Acad Sci U S A, 88, pp.5360~5364 (1991)
- Imai J, "Antioxidant and radical scavenging effects of aged garlic extract and its constituents", Planta Med, 60, pp.417~420 (1994)
- 장은경, "숙성에 의해 제조된 흑 마늘 추출물의 생리학적 활성 및 항산화 효과", 한국식품과학회지, 40(4), pp.443~448 (2008), 한국식품과학회
- Jung UJ, "Antihyperglycemic and antioxidant properties of caffeic acid in db", db mice, 2006, pp.318, 476~483
- Kaneto H, "Role of oxidative stress, endoplasmic reticulum stress, and c-Jun N-terminal kinase in pancreatic β-cell dysfunction and insulin resistance", Int J Biochem Cell Biol, 37, pp.1595~1608 (2005)
- 강민정, "Effect of garlic with different processing on lipid metabolism in 1% cholesterol fed rats", Journal of the Korean Society of Food Science and Nutrition, 37(2), pp.162~169 (2008), 한국식품영양과학회
- Kang YH, "The nitrite scavenging and electron donating ability of phenolic compounds", Korean Society of Food Science and Technology, 28, pp.232~239 (1996)
- Kanth RV, "Attenuation of streptozotocin-induced oxidative stress in hepatic and intestinal tissues of Wistar rat by methanolic-garlic extract", Acta Diabetol, 45, pp.243~251 (2008)
- King H, "Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections", Diabetes Care, 21, pp.1414~1431 (1998)
- Lean ME, "Dietary flavonols protect diabetic human lymphocytes against oxidative damage to DNA", Diabetes, 48, pp.176~181 (1999)
- Maritim AC, "Diabetes, oxidative stress, and antioxidants: a review", J Biochem Mol Toxicol, 17, pp.24~38 (2003)
- Marklund S, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.469~474 (1974)
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.351~358 (1979)
- Paglia DE, "Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase", J Lab Clin Med, 70, pp.158~169 (1967)
- Mi-Kyung Lee, "Eucommia ulmoides Oliver Leaf Extract Increases Endogenous Antioxidant Activity in Type 2 Diabetic Mice", Journal of Medicinal Food, 9(4), pp.474~479 (2006), 한국식품영양과학회
- Queiroz YS, "Garlic (Allium sativum L.) and ready-to-eat garlic products: In vitro antioxidant activity", Food Chem, 115, pp.371~374 (2009)
- Rahimi R, "A review on the role of antioxidants in the management of diabetes and its complications", Biomed Pharmacother, 59, pp.365~373 (2005)
- Re R, "Antioxidant activity applying an improved abts radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Seo KI, "Effect of curcumin supplementation on blood glucose, plasma insulin, and glucose homeostasis related enzyme activities in diabetic db", db mice, 2008, pp.52, 995~1004
- Yeong-Ju Seo, "Effect of Garlic and Aged Black Garlic on Hyperglycemia and Dyslipidemia in Animal Model of Type 2 Diabetes Mellitus", Journal of Food Science and Nutrition, 14(1), pp.1~7 (2009), 한국식품영양과학회
- 신철호, "스트렙토조토신 유발 당뇨병 백서의 산화스트레스에 대한 S-Allylcysteine의 영향", 대한내분비학회지, 23(2), pp.129~136 (2008), 대한내분비학회
- Sinclair AJ, "An investigation of the relationship between free radical activity and vitamin C metabolism in elderly diabetic subjects with retinopathy", Gerontology, 38, pp.268~274 (1992)
- The Diabetes Control and Complications Trial(DCCT)Research Group, "The effect of intensive treatment of diabetes on the development and progression of long-term complications in the diabetes control in insulin-dependent diabetes mellitus", N Engl J Med, 329, pp.977~986 (1993)
- UK Prospective Diabetes Study (UKPDS) Group, "Tight blood pressure control and risk of macrovascular and microvascular complications in type 2 diabetes", UKPDS, 38, pp.703~713 (1998)
- Youn JY, "Anti-hyperglycemic activity of Commelina communis L.: inhibition of α-glucosidase", Diabetes Res Clin Pract, 66S, pp.S149~S155 (2004)