Cydonia oblonga Miller fruit extract exerts an anti-obesity effect in 3T3-L1 adipocytes by activating the AMPK signaling pathway
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BACKGROUND/OBJECTIVES: The fruit of Cydonia oblonga Miller (COM) is used traditionally in Mediterranean region medicine to prevent or treat obesity, but its mechanism of action is still unclear. Beyond a demonstrated anti-obesity effect, the fruit was tested for the mechanism of adipogenesis in 3T3-L1 preadipocytes. MATERIALS/METHODS: 3T3-L1 preadipocytes were cultured for 8 days with COM fruit extract (COME) at different concentrations (0–600 μg/mL) with adipocyte differentiation medium. The cell viability was measured using an MTT assay; triglyceride (TG) was stained with Oil Red O. The expression levels of the adipogenesis-related genes and protein expression were analyzed by reverse transcription polymerase chain reaction and Western blotting, respectively. RESULTS: COME inhibited intracellular TG accumulation during adipogenesis. A COME treatment in 3T3-L1 cells induced upregulation of the adenosine monophosphate-activated protein kinase (AMPK)α phosphorylation and downregulation of the adipogenic transcription factors, such as sterol regulatory element-binding protein 1c, peroxisome proliferator-activated receptor γ, and CCAAT/enhancer binding protein α. The COME treatment reduced the mRNA expression of fatty acyl synthetase, adenosine triphosphate-citrate lyase, adipocyte protein 2, and lipoprotein lipase. It increased the mRNA expression of hormone-sensitive lipase and carnitine palmitoyltransferase I in 3T3-L1 cells. CONCLUSIONS: COME inhibits adipogenesis via the AMPK signaling pathways. COME may be used to prevent and treat obesity.
Abstract
BACKGROUND/OBJECTIVES: The fruit of Cydonia oblonga Miller (COM) is used traditionally in Mediterranean region medicine to prevent or treat obesity, but its mechanism of action is still unclear. Beyond a demonstrated anti-obesity effect, the fruit was tested for the mechanism of adipogenesis in 3T3-L1 preadipocytes. MATERIALS/METHODS: 3T3-L1 preadipocytes were cultured for 8 days with COM fruit extract (COME) at different concentrations (0–600 μg/mL) with adipocyte differentiation medium. The cell viability was measured using an MTT assay; triglyceride (TG) was stained with Oil Red O. The expression levels of the adipogenesis-related genes and protein expression were analyzed by reverse transcription polymerase chain reaction and Western blotting, respectively. RESULTS: COME inhibited intracellular TG accumulation during adipogenesis. A COME treatment in 3T3-L1 cells induced upregulation of the adenosine monophosphate-activated protein kinase (AMPK)α phosphorylation and downregulation of the adipogenic transcription factors, such as sterol regulatory element-binding protein 1c, peroxisome proliferator-activated receptor γ, and CCAAT/enhancer binding protein α. The COME treatment reduced the mRNA expression of fatty acyl synthetase, adenosine triphosphate-citrate lyase, adipocyte protein 2, and lipoprotein lipase. It increased the mRNA expression of hormone-sensitive lipase and carnitine palmitoyltransferase I in 3T3-L1 cells. CONCLUSIONS: COME inhibits adipogenesis via the AMPK signaling pathways. COME may be used to prevent and treat obesity.
참고문헌 (42)
- Elagizi A, "An overview and update on obesity and the obesity paradox in cardiovascular diseases", Prog Cardiovasc Dis, 61, pp.142~150 (2018)
- Seidell JC, "The global burden of obesity and the challenges of prevention", Ann Nutr Metab, 66(Suppl 2), pp.7~12 (2015)
- Lass A, "Lipolysis-a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores", Prog Lipid Res, 50, pp.14~27 (2011)
- Saponaro C, "The subtle balance between lipolysis and lipogenesis : a critical point in metabolic homeostasis", Nutrients, 7, pp.9453~9474 (2015)
- Adamczak M, "The adipose tissue as an endocrine organ", Semin Nephrol, 33, pp.2~13 (2013)
- Poojashree MJ, "A review on the current drugs and new targets for obesity", J Appl Pharm Res, 8, pp.11~21 (2020)
- Baretić M, "Obesity drug therapy", Minerva Endocrinol, 38, pp.245~254 (2013)
- 강준구, "Anti-Obesity Drugs: A Review about Their Effects and Safety", Diabetes and Metabolism Journal, 36(1), pp.13~25 (2012), 대한당뇨병학회
- Hayamizu K, "Effects of garcinia cambogia(hydroxycitric acid)on visceral fat accumulation : a double-blind, randomized, placebo-controlled trial", Curr Ther Res Clin Exp, 64, pp.551~567 (2003)
- Semwal RB, "A comprehensive scientific overview of Garcinia cambogia", Fitoterapia, 102, pp.134~148 (2015)
- Klaus S, "Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation", Int J Obes, 29, pp.615~623 (2005)
- Wang H, "Effects of catechin enriched green tea on body composition", Obesity(Silver Spring), 18, pp.773~779 (2010)
- Abdollahi H., "A review on history, domestication and germplasm collections of quince (Cydonia oblonga Mill.) in the world", Genet Resour Crop Evol, 66, pp.1041~1058 (2019)
- Zhou WT, "Effect of Cydonia oblonga Mill. leaf extracts or captopril on blood pressure and related biomarkers in renal hypertensive rats", J Ethnopharmacol, 153, pp.635~640 (2014)
- Ashraf MU, "A medicinal plant rich in phytonutrients for pharmaceuticals", Front Pharmacol, 7, pp.163~ (2016)
- Silva BM, "Phenolic profile of quince fruit(Cydonia oblonga Miller)(pulp and peel)", J Agric Food Chem, 50, pp.4615~4618 (2002)
- Wojdyło A, "Polyphenolic composition, antioxidant activity, and polyphenol oxidase(PPO)activity of quince(Cydonia oblonga Miller)varieties", J Agric Food Chem, 61, pp.2762~2772 (2013)
- Essafi-Benkhadir K, "Quince(Cydonia oblonga Miller)peel polyphenols modulate LPS-induced inflammation in human THP-1-derived macrophages through NF-κB, p38MAPK and Akt inhibition", Biochem Biophys Res Commun, 418, pp.180~185 (2012)
- Pacifico S, "Antioxidant properties and cytotoxic effects on human cancer cell lines of aqueous fermented and lipophilic quince (Cydonia oblonga Mill.) preparations", Food Chem Toxicol, 50, pp.4130~4135 (2012)
- Adiban H, "Chemopreventive effect of quince (Cydonia oblonga Mill.) fruit extract on hepatocellular carcinoma induced by diethylnitrosamine in rats", Int Pharm Acta, 2, pp.e2~ (2019)
- Mirmohammadlu M, "Hypolipidemic, hepatoprotective and renoprotective effects of Cydonia oblonga Mill. fruit in streptozotocininduced diabetic rats", Iran J Pharm Res, 14, pp.1207~1214 (2015)
- Zhou W, "Effect of Cydonia oblonga Mill. fruit and leaf extracts on blood pressure and blood rheology in renal hypertensive rats", J Ethnopharmacol, 152, pp.464~469 (2014)
- Umar A, "Effects of Cydonia oblonga Miller leaf and fruit flavonoids on blood lipids and anti-oxydant potential in hyperlipidemia rats", J Ethnopharmacol, 169, pp.239~243 (2015)
- Lee HS, "Anti-obesity effect of Cydonia oblonga Miller extract in high-fat diet-induced obese C57BL", 6 mice, 2022, pp.89, 104945~
- Denizot F, "Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability", J Immunol Methods, 89, pp.271~277 (1986)
- Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
- Lim SM, "Cyanidine-3-O-galactoside enriched Aronia melanocarpa extract inhibits adipogenesis and lipogenesis via down-regulation of adipogenic transcription factors and their target genes in 3T3-L1 Cells", Food Nutr Sci, 10, pp.128~147 (2019)
- Kim Yoon Hee, "Gynostemma pentaphyllum extract and Gypenoside L enhance skeletal muscle differentiation and mitochondrial metabolism by activating the PGC-1α pathway in C2C12 myotubes", Nutrition Research and Practice, 16(1), pp.14~32 (2022), 한국영양학회[DOI]
- Guru A, "Molecular mechanism of down-regulating adipogenic transcription factors in 3T3-L1 adipocyte cells by bioactive anti-adipogenic compounds", Mol Biol Rep, 48, pp.743~761 (2021)
- Jemai R, "Colocynth(Citrullus colocynthis)seed extracts attenuate adipogenesis by down-regulating PPARγ", SREBP-1c and C, Food Biosci, pp.2020, 33[DOI]
- Khan N, "Analysis of major polyphenolic compounds of Cydonia oblonga Miller(Quince)fruit extract by UPLC-MS", MS and its effect on adipogenesis in 3T3-L1 cells, 2022, pp.9, 167~
- Ko JH, "Effects of Polygonatum sibiricum rhizome ethanol extract in high-fat diet-fed mice", Pharm Biol, 53, pp.563~570 (2015)
- Horton JD, "Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes", Proc Natl Acad Sci U S A, 100, pp.12027~12032 (2003)
- Gambero A, "The positive effects of yerba maté(Ilex paraguariensis)in obesity", Nutrients, 7, pp.730~750 (2015)
- Wong RHF, "Insulin signaling in fatty acid and fat synthesis : a transcriptional perspective", Curr Opin Pharmacol, 10, pp.684~691 (2010)
- Guay C, "A role for ATP-citrate lyase, malic enzyme, and pyruvate", citrate cycling in glucose-induced insulin secretion, 2007, pp.282, 35657~35665
- Maeda K, "Adipocyte", macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes, 2005, pp.1, 107~119
- de Ferranti S, "The perfect storm : obesity, adipocyte dysfunction, and metabolic consequences", Clin Chem, 54, pp.945~955 (2008)
- Mukherjee B, "Obesity and insulin resistance : an abridged molecular correlation", Lipid Insights, 6, pp.1~11 (2013)
- Woods A, "Liver-specific activation of AMPK prevents steatosis on a high-fructose diet", Cell Reports, 18, pp.3043~3051 (2017)
- Lee J, "Oral intake of Lactobacillus plantarum L-14 extract alleviates TLR2- and AMPK-mediated obesity-associated disorders in high-fat-diet-induced obese C57BL", 6J mice, 2021, pp.54, e13039~
- 전상민, "Regulation and function of AMPK in physiology and diseases", Experimental and Molecular Medicine, 48, pp.1~13 (2016), 생화학분자생물학회