Peanut sprouts extract (Arachis hypogaea L.) has anti-obesity effects by controlling the protein expressions of PPARγ and adiponectin of adipose tissue in rats fed high-fat diet
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BACKGROUD/OBEJECTIVES: This study aims to find out the effects of peanut sprout extracts on weight controls and proteinexpressions of transcription factors related to adipocyte differentiation and adipocytokine in rats under high-fat diets. MATERIALS/METHODS: Four week-old Sparague-Dawley (SD) were assigned to 4 groups; normal-fat (NF) diets (7% fat diet),high-fat (HF) diets (20% fat diet), high fat diets with low peanut sprout extract (HF + PSEL) diet (20% fat and 0.025% peanutsprout extract), and high fat diets with high peanut sprout extract (HF + PSEH) diet (20% fat and 0.05% peanut sprout extract). Body weight changes, lipid profiles in adipose tissue, and the mRNA protein expressions, such as peroxisome proliferator-activatedreceptor γ (PPARγ), CCAAT element binding protein α (C/EBP α), leptin, and adiponectin, were determined. RESULTS: After 9 weeks of feeding, the HF + PSEH group had significantly less weight gains than the HF group (P < 0.05). However, the total dietary intakes or food efficiency ratios among groups were not significantly different. The weight of epididymalfat in HF + PSEH group, 3.61 ± 0.5 g, or HF + PSEL group, 3.80 ± 0.7 g, was significantly lower than the HF group, 4.39 ± 0.4g,(P < 0.05). Total lipids and total cholesterol in adipose tissue were significantly decreased in HF + PSEH group compared tothose in the HF group, respectively (P < 0.05). PSEH supplementation caused AST and ALT levels to decrease when it comparedto HF group, but it was not statistically significant. The protein expression of PPARγ in HF + PSEH group was significantlylower than the HF group (P < 0.05). Comparing with the HF group, the protein expression of adiponectin in HF + PSEH groupwas significantly increased (P < 0.05). The protein expressions of C/EBP α and leptin in HF + PSEH group were lower than theHF group, but it was not statistical significant. CONCLUSIONS: In conclusion, peanut sprout extract has anti-obesity effect by lowering the expressions of PPARγ which regulatesthe expression of adiponectin.
Abstract
BACKGROUD/OBEJECTIVES: This study aims to find out the effects of peanut sprout extracts on weight controls and proteinexpressions of transcription factors related to adipocyte differentiation and adipocytokine in rats under high-fat diets. MATERIALS/METHODS: Four week-old Sparague-Dawley (SD) were assigned to 4 groups; normal-fat (NF) diets (7% fat diet),high-fat (HF) diets (20% fat diet), high fat diets with low peanut sprout extract (HF + PSEL) diet (20% fat and 0.025% peanutsprout extract), and high fat diets with high peanut sprout extract (HF + PSEH) diet (20% fat and 0.05% peanut sprout extract). Body weight changes, lipid profiles in adipose tissue, and the mRNA protein expressions, such as peroxisome proliferator-activatedreceptor γ (PPARγ), CCAAT element binding protein α (C/EBP α), leptin, and adiponectin, were determined. RESULTS: After 9 weeks of feeding, the HF + PSEH group had significantly less weight gains than the HF group (P < 0.05). However, the total dietary intakes or food efficiency ratios among groups were not significantly different. The weight of epididymalfat in HF + PSEH group, 3.61 ± 0.5 g, or HF + PSEL group, 3.80 ± 0.7 g, was significantly lower than the HF group, 4.39 ± 0.4g,(P < 0.05). Total lipids and total cholesterol in adipose tissue were significantly decreased in HF + PSEH group compared tothose in the HF group, respectively (P < 0.05). PSEH supplementation caused AST and ALT levels to decrease when it comparedto HF group, but it was not statistically significant. The protein expression of PPARγ in HF + PSEH group was significantlylower than the HF group (P < 0.05). Comparing with the HF group, the protein expression of adiponectin in HF + PSEH groupwas significantly increased (P < 0.05). The protein expressions of C/EBP α and leptin in HF + PSEH group were lower than theHF group, but it was not statistical significant. CONCLUSIONS: In conclusion, peanut sprout extract has anti-obesity effect by lowering the expressions of PPARγ which regulatesthe expression of adiponectin.
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