The effects of Brassica juncea L. leaf extract on obesity and lipid profiles of rats fed a high-fat/high-cholesterol diet
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BACKGROUND/OBJECTIVES: Obesity is a global health problem of significant importance which increases mortality. In place of anti-obesity drugs, natural products are being developed as alternative therapeutic materials. In this study, we investigated the effect of Brassica juncea L. leaf extract (BLE) on fat deposition and lipid profiles in high-fat, high-cholesterol diet (HFC)-induced obese rats. MATERIALS/METHODS: Male Sprague-Dawley rats were divided into four groups (n = 8 per group) according to diet: normal diet group (ND), high-fat/high-cholesterol diet group (HFC), HFC with 3% BLE diet group (HFC-A1), and HFC with 5% BLE diet group (HFC-A2). Each group was fed for 6 weeks. Rat body and adipose tissue weights, serum biochemical parameters, and tissue lipid contents were determined. The expression levels of mRNA and proteins involved in lipid and cholesterol metabolism were determined by reverse transcription polymerase chain reaction and western blot analysis, respectively. RESULTS: The HFC-A2 group showed significantly lower body weight gain and food efficiency ratio than the HFC group. BLE supplementation caused mesenteric, epididymal, and total adipose tissue weights to decrease. The serum levels of triglyceride, total cholesterol, and low-density lipoprotein cholesterol were significantly reduced, and high-density lipoprotein cholesterol was significantly increased in rats fed BLE. These results were related to lower glucose-6-phosphate dehydrogenase, acetyl-coA carboxylase, and fatty acid synthase mRNA expression, and to higher expression of the cholesterol 7α-hydroxylase and low density lipoprotein-receptor, as well as increased protein levels of peroxisome proliferator-activated receptor α. Histological analysis of the liver revealed decreased lipid droplets in HFC rats treated with BLE. CONCLUSIONS: Supplementation of HFC with 3% or 5% BLE inhibited body fat accumulation, improved lipid profiles, and modulated lipogenesis- and cholesterol metabolism-related gene and protein expression.
Abstract
BACKGROUND/OBJECTIVES: Obesity is a global health problem of significant importance which increases mortality. In place of anti-obesity drugs, natural products are being developed as alternative therapeutic materials. In this study, we investigated the effect of Brassica juncea L. leaf extract (BLE) on fat deposition and lipid profiles in high-fat, high-cholesterol diet (HFC)-induced obese rats. MATERIALS/METHODS: Male Sprague-Dawley rats were divided into four groups (n = 8 per group) according to diet: normal diet group (ND), high-fat/high-cholesterol diet group (HFC), HFC with 3% BLE diet group (HFC-A1), and HFC with 5% BLE diet group (HFC-A2). Each group was fed for 6 weeks. Rat body and adipose tissue weights, serum biochemical parameters, and tissue lipid contents were determined. The expression levels of mRNA and proteins involved in lipid and cholesterol metabolism were determined by reverse transcription polymerase chain reaction and western blot analysis, respectively. RESULTS: The HFC-A2 group showed significantly lower body weight gain and food efficiency ratio than the HFC group. BLE supplementation caused mesenteric, epididymal, and total adipose tissue weights to decrease. The serum levels of triglyceride, total cholesterol, and low-density lipoprotein cholesterol were significantly reduced, and high-density lipoprotein cholesterol was significantly increased in rats fed BLE. These results were related to lower glucose-6-phosphate dehydrogenase, acetyl-coA carboxylase, and fatty acid synthase mRNA expression, and to higher expression of the cholesterol 7α-hydroxylase and low density lipoprotein-receptor, as well as increased protein levels of peroxisome proliferator-activated receptor α. Histological analysis of the liver revealed decreased lipid droplets in HFC rats treated with BLE. CONCLUSIONS: Supplementation of HFC with 3% or 5% BLE inhibited body fat accumulation, improved lipid profiles, and modulated lipogenesis- and cholesterol metabolism-related gene and protein expression.
참고문헌 (44)
- Kopelman P, "Health risks associated with overweight and obesity", Obes Rev, 8(Suppl 1), pp.13~17 (2007)[DOI]
- Michalakis K, "The complex interaction between obesity, metabolic syndrome and reproductive axis: a narrative review", Metabolism, 62, pp.457~478 (2013)[DOI]
- Garg SK, "Diabetes and cancer: two diseases with obesity as a common risk factor", Diabetes Obes Metab, 16, pp.97~110 (2014)[DOI]
- Kopelman PG, "Obesity as a medical problem", Nature, 404, pp.635~643 (2000)[DOI]
- Karelis AD, "Metabolic and body composition factors in subgroups of obesity: what do we know?", J Clin Endocrinol Metab, 89, pp.2569~2575 (2004)[DOI]
- Esteghamati A, "Complementary and alternative medicine for the treatment of obesity: a critical review", Int J Endocrinol Metab, 13, pp.e19678~ (2015)
- Rucker D, "Long term pharma- cotherapy for obesity and overweight: updated meta-analysis", BMJ, 335, pp.1194~1199 (2007)
- Vikas Kumar, "Therapeutic potentials of Brassica juncea: an overview", 셀메드, 1(1), pp.2~2 (2011), 셀메드 세포교정의약학회
- Manohar RP, "Mustard and its uses in Ayurveda", Indian J Tradit Knowl, 8, pp.400~404 (2009)
- Kumar S, "Health promoting bioactive phytochemicals from Brassica", Int Food Res J, 19, pp.59~71 (2012)
- 이주민, "건조방법에 따른 적겨자잎의 이화학적 성분 및 항산화효과 비교", 한국지역사회생활과학회지, 28(4), pp.515~524 (2017), 한국지역사회생활과학회[DOI]
- Hill CB, "Variation in glucosi- nolates in oriental Brassica vegetables", J Am Soc Hortic Sci, 112, pp.309~313 (1987)
- McNaughton SA, "Development of a food composition database for the estimation of dietary intakes of glucosinolates, the biologically active constituents of cruciferous vegetables", Br J Nutr, 90, pp.687~697 (2003)[DOI]
- Miyata S, "Allyl isothiocyanate suppresses the proteolytic activation of sterol regulatory element-binding proteins and de novo fatty acid and cholesterol synthesis", Biosci Biotechnol Biochem, 80, pp.1006~1011 (2016)[DOI]
- Choi KM, "Sulforaphane attenuates obesity by inhibiting adipogenesis and activating the AMPK pathway in obese mice", J Nutr Biochem, 25, pp.201~207 (2014)[DOI]
- Thakur AK, "Antidepressant-like effects of Brassica juncea L. leaves in diabetic rodents", Indian J Exp Biol, 52, pp.613~622 (2014)
- Kim HY, "In vitro and in vivo antioxidant effects of mustard leaf (Brassica juncea)", Phytother Res, 17, pp.465~471 (2003)[DOI]
- Yokozawa T, "Protective effects of mustard leaf (Brassica juncea) against diabetic oxidative stress", J Nutr Sci Vitaminol (Tokyo), 49, pp.87~93 (2003)[DOI]
- Valvala VK, "Effect of mustard (Brassica juncea) leaf extract on streptozotocin- induced diabetic cataract in Wistar rats", J Food Biochem, 35, pp.109~124 (2011)[DOI]
- Kwak Y, "Anti-cancer activities of Brassica juncea leaves in vitro", EXCLI J, 15, pp.699~710 (2016)
- Friedewald WT, "Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge", Clin Chem, 18, pp.499~502 (1972)
- Rosenfeld L, "Lipoprotein analysis. Early methods in the diagnosis of atherosclerosis", Arch Pathol Lab Med, 113, pp.1101~1110 (1989)
- Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
- Wease DF, "New system for automated extraction and simultaneous determination of serum cholesterol and triglycerides", Clin Chem, 21, pp.1430~1436 (1975)
- Zlatkis A, "Study of a new cholesterol reagent", Anal Biochem, 29, pp.143~148 (1969)[DOI]
- Haglund O, "The effects of fish oil on triglycerides, cholesterol, fibrinogen and malondia- ldehyde in humans supplemented with vitamin E", J Nutr, 121, pp.165~169 (1991)[DOI]
- Khan BA, "Role of Murraya koenigii (curry leaf) and Brassica juncea (mustard) in lipid peroxidation", Indian J Physiol Pharmacol, 40, pp.155~158 (1996)
- Yadav SP, "Brassica juncea (Rai) significantly prevented the development of insulin resistance in rats fed fructose-enriched diet", J Ethnopharmacol, 93, pp.113~116 (2004)[DOI]
- Park Y, "Structure-activity relationship of conjugated linoleic acid and its cognates in inhibiting heparin-releasable lipoprotein lipase and glycerol release from fully differentiated 3T3-L1 adipocytes", J Nutr Biochem, 15, pp.561~568 (2004)[DOI]
- Rinella ME, "Nonalcoholic fatty liver disease: a systematic review", JAMA, 313, pp.2263~2273 (2015)[DOI]
- Panchal SK, "High-carbohydrate high-fat diet-induced metabolic syndrome and cardiovascular remodeling in rats", J Cardiovasc Pharmacol, 57, pp.51~64 (2011)
- Huang GJ, "Protective effect of antrosterol from Antrodia camphorata submerged whole broth against carbon tetrachloride-induced acute liver injury in mice", Food Chem, 132, pp.709~716 (2012)[DOI]
- Cousin B, "A role for preadipocytes as macrophage-like cells", FASEB J, 13, pp.305~312 (1999)[DOI]
- Tiniakos DG, "Nonalcoholic fatty liver disease: pathology and pathogenesis", Annu Rev Pathol, 5, pp.145~171 (2010)[DOI]
- Bechmann LP, "The interaction of hepatic lipid and glucose metabolism in liver diseases", J Hepatol, 56, pp.952~964 (2012)
- Beck F, "The ontogeny of peroxisome-proliferator-activated receptor gene expression in the mouse and rat", Proc Biol Sci, 247, pp.83~87 (1992)[DOI]
- Costet P, "Peroxisome proliferator-activated receptor <TEX>${\alpha}$<", TEX>-isoform deficiency leads to progressive dyslipidemia with sexually dimorphic obesity and steatosis, 1998, pp.273, 29577~29585
- Astrup A, "Failure to increase lipid oxidation in response to increasing dietary fat content in formerly obese women", Am J Physiol, 266, pp.592~599 (1994)
- Goldstein JL, "The LDL receptor", Arterioscler Thromb Vasc Biol, 29, pp.431~438 (2009)[DOI]
- Reena MB, "Enhanced hypocholesterolemic effects of interesterified oils are mediated by upregulating LDL receptor and cholesterol 7-<TEX>${\alpha}$<", TEX>-hydroxylase gene expression in rats, 2011, pp.141, 24~30
- Chiang JY, "Bile acids: regulation of synthesis", J Lipid Res, 50, pp.1955~1966 (2009)[DOI]
- Hebanowska A, "Mechanisms of bile acid biosynthesis regulation- autoregulation by bile acids", Postepy Biochem, 57, pp.314~323 (2011)
- Li T, "Transgenic expression of cholesterol <TEX>$7{\alpha}$<", TEX>-hydroxylase in the liver prevents high-fat diet-induced obesity and insulin resistance in mice, 2010, pp.52, 678~690
- Miyake JH, "Transgenic expression of cholesterol-7-<TEX>${\alpha}$<", TEX>-hydroxylase prevents atherosclerosis in C57BL, Arterioscler Thromb Vasc Biol, pp.2002, 22[DOI]
