Comparison of the gut microbiota profile in breast-fed and formula-fed Korean infants using pyrosequencing
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BACKGROUND/OBJECTIVES: Feeding in infancy is the most significant determinant of the intestinal microbiota in early life. The aim of this study was to determine the gut microbiota of Korean infants and compare the microbiota obtained between breast-fed and formula-fed Korean infants. SUBJECTS/METHODS: We analyzed the microbial communities in fecal samples collected from twenty 4-week old Korean (ten samples in each breast-fed or formula-fed) infants using pyrosequencing. RESULTS: The fecal microbiota of the 4-week-old Korean infants consisted of the three phyla Actinobacteria, Firmicutes, and Proteobacteria. In addition, five species, including Bifidocbacterium longum, Streptococcus salivarius, Strepotococcus lactarius, Streptococcus pseudopneumoniae, and Lactobacillus gasseri were common commensal intestinal microbiota in all infants. The predominant intestinal microbiota in the breast-fed infants (BFI) included the phylum Actinobacteria (average 70.55%), family Bifidobacteriacea (70.12%), genus Bifidobacterium (70.03%) and species Bifidobacterium longum (69.96%). In the microbiota from the formula-fed infants (FFI), the proportion of the phylum Actinobacteria (40.68%) was less, whereas the proportions of Firmicutes (45.38%) and Proteobacteria (13.85%) as well as the diversity of each taxonomic level were greater, compared to those of the BFI. The probiotic species found in the 4-week-old Korean infants were Bifidobacterium longum, Streptococcus salivarius, and Lactobacillus gasseri. These probiotic species accounted for 93.81% of the microbiota from the BFI, while only 63.80% of the microbiota from the FFI. In particular, B. longum was more abundant in BFI (69.96%) than in FFI (34.17%). CONCLUSIONS: Breast milk supports the growth of B. longum and inhibits others. To the best of our knowledge, this study was the first attempt to analyze the gut microbiota of healthy Korean infants according to the feeding type using pyrosequencing. Our data can be used as a basis for further studies to investigate the development of intestinal microbiota with aging and disease status.
Abstract
BACKGROUND/OBJECTIVES: Feeding in infancy is the most significant determinant of the intestinal microbiota in early life. The aim of this study was to determine the gut microbiota of Korean infants and compare the microbiota obtained between breast-fed and formula-fed Korean infants. SUBJECTS/METHODS: We analyzed the microbial communities in fecal samples collected from twenty 4-week old Korean (ten samples in each breast-fed or formula-fed) infants using pyrosequencing. RESULTS: The fecal microbiota of the 4-week-old Korean infants consisted of the three phyla Actinobacteria, Firmicutes, and Proteobacteria. In addition, five species, including Bifidocbacterium longum, Streptococcus salivarius, Strepotococcus lactarius, Streptococcus pseudopneumoniae, and Lactobacillus gasseri were common commensal intestinal microbiota in all infants. The predominant intestinal microbiota in the breast-fed infants (BFI) included the phylum Actinobacteria (average 70.55%), family Bifidobacteriacea (70.12%), genus Bifidobacterium (70.03%) and species Bifidobacterium longum (69.96%). In the microbiota from the formula-fed infants (FFI), the proportion of the phylum Actinobacteria (40.68%) was less, whereas the proportions of Firmicutes (45.38%) and Proteobacteria (13.85%) as well as the diversity of each taxonomic level were greater, compared to those of the BFI. The probiotic species found in the 4-week-old Korean infants were Bifidobacterium longum, Streptococcus salivarius, and Lactobacillus gasseri. These probiotic species accounted for 93.81% of the microbiota from the BFI, while only 63.80% of the microbiota from the FFI. In particular, B. longum was more abundant in BFI (69.96%) than in FFI (34.17%). CONCLUSIONS: Breast milk supports the growth of B. longum and inhibits others. To the best of our knowledge, this study was the first attempt to analyze the gut microbiota of healthy Korean infants according to the feeding type using pyrosequencing. Our data can be used as a basis for further studies to investigate the development of intestinal microbiota with aging and disease status.
참고문헌 (36)
- Simon GL, "Intestinal flora in health and disease", Gastroenterology, 86, pp.174~193 (1984)
- Young VB, "The intestinal microbiota in health and disease", Curr Opin Gastroenterol, 28, pp.63~69 (2012)
- De Filippo C, "Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa", Proc Natl Acad Sci U S A, 107, pp.14691~14696 (2010)
- Cardona F, "Benefits of polyphenols on gut microbiota and implications in human health", J Nutr Biochem, 24, pp.1415~1422 (2013)
- Arumugam M, "Enterotypes of the human gut microbiome", Nature, 473, pp.174~180 (2011)
- Wu GD, "Linking long-term dietary patterns with gut microbial enterotypes", Science, 334, pp.105~108 (2011)
- Fallani M, "Intestinal microbiota of 6-week-old infants across Europe: geographic influence beyond delivery mode, breastfeeding, and antibiotics", J Pediatr Gastroenterol Nutr, 51, pp.77~84 (2010)
- Morelli L, "Postnatal development of intestinal microflora as influenced by infant nutrition", J Nutr, 138, pp.1791S~1795S (2008)
- Hooper LV, "Bacterial contributions to mammalian gut development", Trends Microbiol, 12, pp.129~134 (2004)
- Penders J, "Factors influencing the composition of the intestinal microbiota in early infancy", Pediatrics, 118, pp.511~521 (2006)
- Hanson LA, "The role of breastfeeding in prevention of neonatal infection", Semin Neonatol, 7, pp.275~281 (2002)
- López-Alarcón M, "Breast-feeding lowers the frequency and duration of acute respiratory infection and diarrhea in infants under six months of age", J Nutr, 127, pp.436~443 (1997)
- Morrow AL, "Human milk protection against infectious diarrhea: implications for prevention and clinical care", Semin Pediatr Infect Dis, 15, pp.221~228 (2004)
- Wright AL, "Increasing breastfeeding rates to reduce infant illness at the community level", Pediatrics, 101, pp.837~844 (1998)
- Isaacs CE, "Human milk inactivates pathogens individually, additively, and synergistically", J Nutr, 135, pp.1286~1288 (2005)
- Turnbaugh PJ, "A core gut microbiome in obese and lean twins", Nature, 457, pp.480~484 (2009)
- Zaura E, "Defining the healthy "core microbiome" of oral microbial communities", BMC Microbiol, 9, pp.259~ (2009)
- Kim BS, "Impact of enrofloxacin on the human intestinal microbiota revealed by comparative molecular analysis", Anaerobe, 18, pp.310~320 (2012)
- Jeon YS, "Identification of household bacterial community and analysis of species shared with human microbiome", Curr Microbiol, 67, pp.557~563 (2013)
- Kim OS, "Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species", Int J Syst Evol Microbiol, 62, pp.716~721 (2012)
- Edgar RC, "UCHIME improves sensitivity and speed of chimera detection", Bioinformatics, 27, pp.2194~2200 (2011)
- Schloss PD, "Introducing mothur:open-source, platform-independent, community-supported software for describing and comparing microbial communities", Appl Environ Microbiol, 75, pp.7537~7541 (2009)
- Rijkers GT, "Health benefits and health claims of probiotics: bridging science and marketing", Br J Nutr, 106, pp.1291~1296 (2011)
- Chang JY, "Pyrosequencing-based molecular monitoring of the intestinal bacterial colonization in preterm infants", J Pediatr Gastroenterol Nutr, 53, pp.512~519 (2011)
- Heikkilä MP, "Inhibition of Staphylococcus aureus by the commensal bacteria of human milk", J Appl Microbiol, 95, pp.471~478 (2003)
- Wenguang Fan, "Impact of Diet in Shaping Gut Microbiota Revealed by a Comparative Study in Infants During the First Six Months of Life", Journal of Microbiology and Biotechnology, 24(2), pp.133~143 (2014), 한국미생물·생명공학회[DOI]
- Bezirtzoglou E, "Microbiota profile in feces of breast- and formula-fed newborns by using fluorescence in situ hybridization (FISH)", Anaerobe, 17, pp.478~482 (2011)
- Tannock GW, "Comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk", Appl Environ Microbiol, 79, pp.3040~3048 (2013)
- Yoshioka H, "Development and differences of intestinal flora in the neonatal period in breast-fed and bottle-fed infants", Pediatrics, 72, pp.317~321 (1983)
- Lilly DM, "Probiotics: growth-promoting factors produced by microorganisms", Science, 147, pp.747~748 (1965)
- Coppa GV, "Oligosaccharides in 4 different milk groups, Bifidobacteria, and Ruminococcus obeum", J Pediatr Gastroenterol Nutr, 53, pp.80~87 (2011)
- Marcobal A, "Human milk oligosaccharide consumption by intestinal microbiota", Clin Microbiol Infect, 18(Suppl 4), pp.12~15 (2012)
- Conway PL, "Selection criteria for probiotic microorganisms", Asia Pac J Clin Nutr, 5, pp.10~14 (1996)
- Bezirtzoglou E, "Regulation of the bacterial intestinal implantation in infant born by caesarean section", Comp Immunol Microbiol Infect Dis, 15, pp.71~74 (1992)
- Thum C, "Can nutritional modulation of maternal intestinal microbiota influence the development of the infant gastrointestinal tract?", J Nutr, 142, pp.1921~1928 (2012)
- Hascoët JM, "Effect of formula composition on the development of infant gut microbiota", J Pediatr Gastroenterol Nutr, 52, pp.756~762 (2011)