Pyrosequencing 분석을 이용한 신생아 장내 미생물 군집 프로파일 분석: 예비연구
Intestinal Microbial Community Profiles of a Newborn Preterm Infant Using Pyrosequencing Analysis: Pilot Study
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초록
DNA 서열 결정을 위한 진보된 방법 가운데 하나인 DNA pyrosequencing은, 16S rRNA 유전자를 이용한 세균 군집의 계통발생학적 분석에 이용되는 기법이다. 이러한 DNA pyrosequencing을 이용하여, 저자들은 한 쌍의 산모-신생아 쌍을 대상으로 산모의 양수, 질, 직장, 그리고 신생아의 위액 및 대변에서 각각 미생물 군집의 다양성을 조사하였다. 문(phylum) 수준에서의 분석 결과, 산모의 대변에서는 Firmicutes (63.2%)가, 질에서는 Actinobacteria (84.9%)가 주를 이루고 있었고, 산모의 양수와 그 신생아의 위액에서는 Proteobacteria (67.8%)가 주를 이루고 있었다. 흥미로운 것은 신생아 태변 내 세균 군집의 양상이 양수의 것과 상당히 유사하다는 것이었다. 그러나 생후 2주된 신생아의 대변 내의 양상은 사뭇 달라서 Firmicutes (91.1%)가 주를 이루고 있었다. 속 (genus) 수준의 분석에서는 산모의 대변 내 세균 군집은Anaerococcus (19.5%) and Prevotella (18.7%)가 주를 이루었으나, 질에서는 Atopobium (83.6%)가 주를 이루었다. 산모의 양수와 신생아의 위액에서는 Sphingomonas (38.5%)가 주를 이루었다. 신생아 태변 내 세균 군집의 양상은 양수의것과 상당히 유사하였다. 그러나 생후 2주된 신생아의 대변에서는 상당히 다른 양상을 보였다. 신생아의 대변 내 세균군집의 변화를 알기 위해서는 향후 보다 많은 수를 대상으로 하는 연구가 필요하다고 판단된다.
Abstract
DNA pyrosequencing, one of the advanced methods for DNA sequencing, has beenemployed for phylogenetic analysis of bacterial communities using the conserved16S rRNA gene. We performed a pilot study on a mother-neonate pair utilizing theDNA pyrosequencing assays to investigate the diversity of microbial communitiesin maternal amniotic fluid (AF), vagina, and rectum and newborn gastric fluid (GF)and stool. Phylum level analysis revealed that bacterial community was dominatedby Firmicutes (63.2%) in maternal feces, and Actinobacteria (84.9%) in maternalvaginal swab. The bacterial communities in both the AF and GF were dominatedby Proteobacteria (67.8%). Interestingly, the bacterial community in the newborn’smeconium was quite similar to that in the AF. However, the composition of the bacterialcommunity in newborn’s feces was different on day 14 and dominated by Firmicutes(91.1%). Genus-level analysis revealed that the bacterial community in maternalfeces was dominated by Anaerococcus (19.5%) and Prevotella (18.7%), whereas thatin the maternal vaginal swab was dominated by Atopobium (83.6%). The bacterialcommunities in both the AF and GF were dominated by Sphingomonas (38.5%). The bacterial community in the newborn’s meconium was quite similar to that inthe AF, which was dominated by Sphingomonas (45.2%). However, the compositionof bacterial community in the newborn’s feces on day 14 was relatively different. Future studies with a large number of infants are needed to determine the factorsinvolved in the changing profile of newborn’s fecal bacterial communities.
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