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Carthamus tinctorius seeds–Taraxacum coreanum combination attenuates scopolamine-induced memory deficit through regulation of inflammatory response and cholinergic function

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BACKGROUND/OBJECTIVES: There is growing interest in herbal medicines for managing age-related diseases, such as Alzheimer's and Parkinson’s. Safflower seeds (Carthamus tinctorius L. seeds, CTS) and dandelions (Taraxacum coreanum, TC) are widely used to treat bone- or inflammation-related diseases in Oriental countries. This study investigated the protective effect of the CTS–TC combination on scopolamine (Sco)-induced memor y deficits through inflammator y response and cholinergic function. Moreover, marker components such as serotonin, N-(p-coumaroyl) serotonin, N-feruloylserotonin, chlorogenic acid, and chicoric acid in the CTS–TC combination were analyzed for their potential benefits on memor y function. MATERIALS/METHODS: Water extracts of CTS, TC, and the CTS–TC combination at various ratios (4:1, 1:1, and 1:4) (100 mg/kg) were orally administered to mice for 14 days. Sco (1 mg/kg) was intraperitoneally injected into the mice before each behavioral test. T-maze and novel object recognition tests were conducted to monitor behavioral changes after the treatment. Western blotting was performed to detect protein expression. In addition, the presence of 5 biomarkers, serotonin, N-(p-coumaroyl) serotonin, N-feruloylserotonin, chlorogenic acid, and chicoric acid, was analyzed using high-performance liquid chromatography (HPLC). RESULTS: Behavioral tests showed that the CTS–TC combination enhanced memor y function in Sco-injected mice. Inflammation-related proteins (inducible nitric oxide synthase, cyclooxygenase-2, and glial fibrillar y acidic protein) were downregulated after treatment with the CTS–TC combination. The acetylcholinesterase protein expression was also downregulated. HPLC analysis revealed that N-feruloylserotonin and chicoric acid were the predominant components, followed by N-(p-coumaroyl) serotonin, chlorogenic acid, and serotonin. CONCLUSION: These findings suggest that the CTS–TC combination protects againstSco-induced memor y deficits by inhibiting inflammator y responses and cholinergic dysfunction. N-feruloylserotonin and chicoric acid, along with N-(p-coumaroyl) serotonin, chlorogenic acid, and serotonin, might be biomarkers for the CTS–TC combination, and their effects on memor y protection warrant further study.

Abstract

BACKGROUND/OBJECTIVES: There is growing interest in herbal medicines for managing age-related diseases, such as Alzheimer's and Parkinson’s. Safflower seeds (Carthamus tinctorius L. seeds, CTS) and dandelions (Taraxacum coreanum, TC) are widely used to treat bone- or inflammation-related diseases in Oriental countries. This study investigated the protective effect of the CTS–TC combination on scopolamine (Sco)-induced memor y deficits through inflammator y response and cholinergic function. Moreover, marker components such as serotonin, N-(p-coumaroyl) serotonin, N-feruloylserotonin, chlorogenic acid, and chicoric acid in the CTS–TC combination were analyzed for their potential benefits on memor y function. MATERIALS/METHODS: Water extracts of CTS, TC, and the CTS–TC combination at various ratios (4:1, 1:1, and 1:4) (100 mg/kg) were orally administered to mice for 14 days. Sco (1 mg/kg) was intraperitoneally injected into the mice before each behavioral test. T-maze and novel object recognition tests were conducted to monitor behavioral changes after the treatment. Western blotting was performed to detect protein expression. In addition, the presence of 5 biomarkers, serotonin, N-(p-coumaroyl) serotonin, N-feruloylserotonin, chlorogenic acid, and chicoric acid, was analyzed using high-performance liquid chromatography (HPLC). RESULTS: Behavioral tests showed that the CTS–TC combination enhanced memor y function in Sco-injected mice. Inflammation-related proteins (inducible nitric oxide synthase, cyclooxygenase-2, and glial fibrillar y acidic protein) were downregulated after treatment with the CTS–TC combination. The acetylcholinesterase protein expression was also downregulated. HPLC analysis revealed that N-feruloylserotonin and chicoric acid were the predominant components, followed by N-(p-coumaroyl) serotonin, chlorogenic acid, and serotonin. CONCLUSION: These findings suggest that the CTS–TC combination protects againstSco-induced memor y deficits by inhibiting inflammator y responses and cholinergic dysfunction. N-feruloylserotonin and chicoric acid, along with N-(p-coumaroyl) serotonin, chlorogenic acid, and serotonin, might be biomarkers for the CTS–TC combination, and their effects on memor y protection warrant further study.

참고문헌 (81)

  1. Mucke L, "Neuroscience : Alzheimer’s disease", Nature, 461, pp.895~897 (2009)
  2. Lane CA, "Alzheimer’s disease", Eur J Neurol, 25, pp.59~70 (2018)
  3. Chang EH, "Cholinergic control of inflammation, metabolic dysfunction, and cognitive impairment in obesity-associated disorders : mechanisms and novel therapeutic opportunities", Front Neurosci, 13, pp.263~ (2019)
  4. Block ML, "Microglia and inflammation-mediated neurodegeneration : multiple triggers with a common mechanism", Prog Neurobiol, 76, pp.77~98 (2005)
  5. Abdelhak A, "Blood GFAP as an emerging biomarker in brain and spinal cord disorders", Nat Rev Neurol, 18, pp.158~172 (2022)
  6. Jurga AM, "Beyond the GFAP-astrocyte protein markers in the brain", Biomolecules, 11, pp.1361~ (2021)
  7. Zhang S, "GFAP expression in injured astrocytes in rats", Exp Ther Med, 14, pp.1905~1908 (2017)
  8. Bettcher BM, "Astrogliosis and episodic memory in late life : higher GFAP is related to worse memory and white matter microstructure in healthy aging and Alzheimer’s disease", Neurobiol Aging, 103, pp.68~77 (2021)
  9. Tang KS, "The cellular and molecular processes associated with scopolamine-induced memory deficit : a model of Alzheimer’s biomarkers", Life Sci, 233, pp.116695~ (2019)
  10. Anand P, "A review on cholinesterase inhibitors for Alzheimer’s disease", Arch Pharm Res, 36, pp.375~399 (2013)
  11. Pepeu G, "The fate of the brain cholinergic neurons in neurodegenerative diseases", Brain Res, 1670, pp.173~184 (2017)
  12. Rosas-Ballina M, "Cholinergic control of inflammation", J Intern Med, 265, pp.663~679 (2009)
  13. Bajo R, "Scopolamine effects on functional brain connectivity : a pharmacological model of Alzheimer’s disease", Sci Rep, 5, pp.9748~ (2015)
  14. Choi JH, "Allium hookeri extracts improve scopolamine-induced cognitive impairment via activation of the cholinergic system and anti-neuroinflammation in mice", Nutrients, 13, pp.2890~ (2021)
  15. Klinkenberg I, "The validity of scopolamine as a pharmacological model for cognitive impairment : a review of animal behavioral studies", Neurosci Biobehav Rev, 34, pp.1307~1350 (2010)
  16. Chen WN, "Scopolamine, a toxin-induced experimental model, used for research in Alzheimer’s disease", CNS Neurol Disord Drug Targets, 19, pp.85~93 (2020)
  17. Wang S, "Compatibility art of traditional Chinese medicine : from the perspective of herb pairs", J Ethnopharmacol, 143, pp.412~423 (2012)
  18. Rolnik A, "The plants of the Asteraceae family as agents in the protection of human health", Int J Mol Sci, 22, pp.3009~ (2021)
  19. Bessada SM, "Asteraceae species with most prominent bioactivity and their potential applications : a review", Ind Crops Prod, 76, pp.604~615 (2015)
  20. Lee HH, "Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extracts", Korean J Med Crop Sci, 16, pp.79~85 (2008)
  21. Park MS, "Antioxidative and anticancer activities of water extracts from different parts of Taraxacum coreanum Nakai cultivated in Korea", J Korean Soc Food Sci Nutr, 44, pp.1234~1240 (2015)
  22. Jin Y, "Herb pairs containing Angelicae Sinensis Radix(Danggui) : a review of bio-active constituents and compatibility effects", J Ethnopharmacol, 181, pp.158~171 (2016)
  23. Jiang M, "An"essential herbal medicine"-licorice : a review of phytochemicals and its effects in combination preparations", J Ethnopharmacol, 249, pp.112439~ (2020)
  24. Shang H, "A method for proportion screening of TCM small prescriptions", Chin J Exp Tradit Med Formulae, 3, pp.1~3 (2003)
  25. Sheng YM, "Optimization of the best ratio of Astragalus polysaccharide and Astragalus saponin for immunomodulation based on increase-decrease baseline geometric proportion design method", Chin J Hosp Pharm, 36, pp.59~62 (2016)
  26. Chen CC, "The"compatible art"of effective components from traditional Chinese medicine : research on the compatibility and proportion of effective components", Acta Pharm Sinica, 54, pp.808~817 (2019)
  27. Mani V, "A metabolic perspective and opportunities in pharmacologically important safflower", Metabolites, 10, pp.253~ (2020)
  28. Akhtar U, "A review on : phytoconstituents, traditional and medicinal uses of Taraxacum offinale(Dandelion)", J Pharmacogn Phytochem, 15, pp.139~144 (2023)
  29. Mahboubi M, "Hepatoprotection by dandelion(Taraxacum officinale)and mechanisms", Asian Pac J Trop Biomed, 10, pp.1~10 (2020)
  30. Kim JH, "Safflower(Carthamus tinctorius L. )seed attenuates memory impairment induced by scopolamine in mice via regulation of cholinergic dysfunction and oxidative stress", Food Funct, 10, pp.3650~3659 (2019)
  31. Kim EJ, "Ginsenosides Rg5 and Rh3 protect scopolamine-induced memory deficits in mice", J Ethnopharmacol, 146, pp.294~299 (2013)
  32. Montgomery KC, "A test of two explanations of spontaneous alternation", J Comp Physiol Psychol, 45, pp.287~293 (1952)
  33. Bevins RA, "Object recognition in rats and mice : a one-trial non-matching-to-sample learning task to study ‘recognition memory’", Nat Protoc, 1, pp.1306~1311 (2006)
  34. Barmak MJ, "Safflower seed oil, a rich source of linoleic acid, stimulates hypothalamic neurogenesis in vivo", Anat Cell Biol, 56, pp.219~227 (2023)
  35. Liang Y, "Carthamus tinctorius L. : a natural neuroprotective source for anti-Alzheimer’s disease drugs", J Ethnopharmacol, 298, pp.115656~ (2022)
  36. He MT, "Combination of Carthamus tinctorius L. seed and Taraxacum coreanum exerts synergistic effects on learning and memory function by regulating metabolism of amyloid beta in mice", J Funct Foods, 72, pp.104048~ (2020)
  37. Dongare PN, "Review on Pharmacognosy, phytochemistry and pharmacological activity of Taraxacum officinale(Dandelion plant)", World J Adv Res Rev, 12, pp.212~218 (2021)
  38. Jalili C, "An overview of therapeutic potentials of Taraxacum officinale(dandelion) : a traditionally valuable herb with a reach historical background", World Cancer Res J, 7, pp.e1679~ (2020)
  39. Yoon CS, "Taraxacum coreanum protects against glutamate-induced neurotoxicity through heme oxygenase-1 expression in mouse hippocampal HT22 cells", Mol Med Rep, 15, pp.2347~2352 (2017)
  40. Lee AY, "Cognition and memory function of Taraxacum coreanum in an in vivo amyloid-β-induced mouse model of Alzheimer’s disease", Arch Biol Sci, 66, pp.1357~1366 (2014)
  41. Sohn MK, "A comparative study of memory improving effects of Taraxaci Herba on scopolamine-induced amnesia in mouse", Kor J Herbology, 27, pp.27~35 (2012)
  42. van Asselen M, "Brain areas involved in spatial working memory", Neuropsychologia, 44, pp.1185~1194 (2006)
  43. Falsafi SK, "Scopolamine administration modulates muscarinic, nicotinic and NMDA receptor systems", PLoS One, 7, pp.e32082~ (2012)
  44. Antonova E, "Scopolamine disrupts hippocampal activity during allocentric spatial memory in humans : an fMRI study using a virtual reality analogue of the Morris Water Maze", J Psychopharmacol, 25, pp.1256~1265 (2011)
  45. Yamazaki M, "Novel 5-HT5A receptor antagonists ameliorate scopolamine-induced working memory deficit in mice and reference memory impairment in aged rats", J Pharmacol Sci, 127, pp.362~369 (2015)
  46. Antunes M, "The novel object recognition memory : neurobiology, test procedure, and its modifications", Cogn Process, 13, pp.93~110 (2012)
  47. Can MV, "Willughbeia cochinchinensis prevents scopolamine-induced deficits in memory, spatial learning, and object recognition in rodents", J Ethnopharmacol, 214, pp.99~105 (2018)
  48. Brown MW, "Recognition memory : what are the roles of the perirhinal cortex and hippocampus", Nat Rev Neurosci, 2, pp.51~61 (2001)
  49. Lee YK, "Protective effect of the ethanol extract of Magnolia officinalis and 4-O-methylhonokiol on scopolamine-induced memory impairment and the inhibition of acetylcholinesterase activity", J Nat Med, 63, pp.274~282 (2009)
  50. Deng G, "Ameliorative effect of deoxyvasicine on scopolamine-induced cognitive dysfunction by restoration of cholinergic function in mice", Phytomedicine, 63, pp.153007~ (2019)
  51. Choi SH, "Protective effect of Carthamus tinctorius L. seed on oxidative stress and cognitive impairment induced by chronic alcohol consumption in mice", Food Sci Biotechnol, 27, pp.1475~1484 (2018)
  52. Kwon Y, "Ameliorating effect of Taraxacum platycarpum extract in the scopolamine-induced cholinergic blockade mouse model", Korean J Pharmacogn, 48, pp.119~124 (2017)
  53. Ahmad A, "Total isoflavones from soybean and tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain", Food Chem Toxicol, 65, pp.120~128 (2014)
  54. Li SP, "Analogous β-carboline alkaloids harmaline and harmine ameliorate scopolamine-induced cognition dysfunction by attenuating acetylcholinesterase activity, oxidative stress, and inflammation in mice", Front Pharmacol, 9, pp.346~ (2018)
  55. Agostinho P, "Neuroinflammation, oxidative stress and the pathogenesis of Alzheimer’s disease", Curr Pharm Des, 16, pp.2766~2778 (2010)
  56. Aktan F, "iNOS-mediated nitric oxide production and its regulation", Life Sci, 75, pp.639~653 (2004)
  57. Khurana K, "Lacidipine prevents scopolamine-induced memory impairment by reducing brain oxido-nitrosative stress in mice", Neurotox Res, 39, pp.1087~1102 (2021)
  58. Lee JS, "Hippocampal memory enhancing activity of pine needle extract against scopolamine-induced amnesia in a mouse model", Sci Rep, 5, pp.9651~ (2015)
  59. Khan AA, "Expression of COX-1 and COX-2 in a clinical model of acute inflammation", J Pain, 8, pp.349~354 (2007)
  60. Cui J, "Natural COX-2 inhibitors as promising anti-inflammatory agents : an update", Curr Med Chem, 28, pp.3622~3646 (2021)
  61. Xian YF, "Honokiol improves learning and memory impairments induced by scopolamine in mice", Eur J Pharmacol, 760, pp.88~95 (2015)
  62. Cho BJ, "Effects of root of Taraxacum coreanum Nakai on the inhibition of inflammation and oxidative stress induced by lipopolysaccharide in ICR Mice", J Korean Soc Food Sci Nutr, 44, pp.1763~1770 (2015)
  63. Kassubek R, "GFAP in early multiple sclerosis : a biomarker for inflammation", Neurosci Lett, 657, pp.166~170 (2017)
  64. Memudu AE, "Alpha lipoic acid ameliorates scopolamine induced memory deficit and neurodegeneration in the cerebello-hippocampal cortex", Metab Brain Dis, 36, pp.1729~1745 (2021)
  65. Konar A, "Protective role of Ashwagandha leaf extract and its component withanone on scopolamine-induced changes in the brain and brain-derived cells", PLoS One, 6, pp.e27265~ (2011)
  66. Pereira JB, "Plasma GFAP is an early marker of amyloid-β but not tau pathology in Alzheimer’s disease", Brain, 144, pp.3505~3516 (2021)
  67. Oeckl P, "Serum GFAP differentiates Alzheimer’s disease from frontotemporal dementia and predicts MCI-to-dementia conversion", J Neurol Neurosurg Psychiatry, 93, pp.659~667 (2022)
  68. Liu Q, "Chicoric acid supplementation prevents systemic inflammation-induced memory impairment and amyloidogenesis via inhibition of NF-κB", FASEB J, 31, pp.1494~1507 (2017)
  69. Naveed M, "Chlorogenic acid(CGA) : a pharmacological review and call for further research", Biomed Pharmacother, 97, pp.67~74 (2018)
  70. Kwon SH, "Neuroprotective effects of chlorogenic acid on scopolamine-induced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice", Eur J Pharmacol, 649, pp.210~217 (2010)
  71. Mohammad-Zadeh LF, "Serotonin : a review", J Vet Pharmacol Ther, 31, pp.187~199 (2008)
  72. Sakamura S, "Conjugated serotonins and phenolic constituents in safflower seed(Carthamus tinctorius L. )", Agric Biol Chem, 44, pp.2951~2954 (1980)
  73. He M, "N-feruloyl serotonin attenuates neuronal oxidative stress and apoptosis in Aβ25–35-treated human neuroblastoma SH-SY5Y cells", Molecules, 28, pp.1610~ (2023)
  74. Kim DH, "Serotonins of safflower seeds play a key role in anti-inflammatory effect in lipopolysaccharide-stimulated RAW 264. 7 macrophages. J", Plant Biotechnol, 42, pp.364~369 (2015)
  75. Jung ES, "Inhibitory effects of serotonin derivatives on adipogenesis", J Soc Cosmet Sci Korea, 37, pp.171~176 (2011)
  76. Carola C, "A Cornflower extract containing N-feruloylserotonin reduces inflammation in human skin by neutralizing CCL17 and CCL22 and inhibiting COX-2 and 5-LOX", Mediators Inflamm, 2021, pp.6652791~ (2021)
  77. Han SJ, "Safflower seed extract attenuates the development of osteoarthritis by blocking NF-κB signaling", Pharmaceuticals(Basel), 14, pp.258~ (2021)
  78. Gottlieb A, "Rodent brain growth stages : an analytical review", Biol Neonate, 32, pp.166~176 (1977)
  79. Chen VS, "Histology atlas of the developing prenatal and postnatal mouse central nervous system, with emphasis on prenatal days E7. 5 to E18. 5", Toxicol Pathol, 45, pp.705~744 (2017)
  80. Ciceri G, "An epigenetic barrier sets the timing of human neuronal maturation", Nature, 626, pp.881~890 (2024)
  81. Guo L, "Dynamic rewiring of neural circuits in the motor cortex in mouse models of Parkinson’s disease", Nat Neurosci, 18, pp.1299~1309 (2015)

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