Consumption of poly-γ-glutamate-vitamin B6 supplement and urinary microbiota profiles in Korean healthy adults: a randomized, double-blinded, placebo-controlled intervention study
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BACKGROUND/OBJECTIVES: Poly-γ-glutamic acid (γ-PGA), a natural polymer found in fermented soybean products, has been reported to play a prebiotic role in the gut. Thisinter vention study investigated the effects of γ-PGA-containing supplement consumption on urinar y microbiota in healthy adults because of limited data on such investigation. SUBJECTS/METHODS: A 4-week parallel trial including 39 male and female Korean adults, who were free of chronic diseases and infection, was designed as a randomized, double- blinded, placebo-controlled study. A total of 30 participants completed the study wherein the inter vention group (n = 17) received a mixture supplement containing 600 mg/day of γ-PGA and 100 mg/day of vitamin B6, while the control group (n = 13) received a placebo. Paired datasets (baseline and endpoint data) of microbiota profiles, which were constructed via urinar y assays of microbe-derived extracellular vesicles, were analyzed and compared between the two groups. RESULTS: Only the inter vention group yielded significant results for the Bray-Curtis and Jaccard dissimilarity indices between baseline and endpoint data (P < 0.05). In the phylum- level analysis of microbial composition, the Firmicutes to Bacteroidetes ratio (FB ratio) tended to decrease from baseline in the inter vention group; however, it increased in the control group. Differences between the baseline and endpoint FB ratios were significant between the two groups (P < 0.05). CONCLUSION: This study’s findings suggest that γ-PGA-vitamin B6 supplementation potentially alters the microbial community composition of a host. Further investigation into the biological consequences of commensal microbiota alteration by γ-PGA-containing supplement consumption is warranted.
Abstract
BACKGROUND/OBJECTIVES: Poly-γ-glutamic acid (γ-PGA), a natural polymer found in fermented soybean products, has been reported to play a prebiotic role in the gut. Thisinter vention study investigated the effects of γ-PGA-containing supplement consumption on urinar y microbiota in healthy adults because of limited data on such investigation. SUBJECTS/METHODS: A 4-week parallel trial including 39 male and female Korean adults, who were free of chronic diseases and infection, was designed as a randomized, double- blinded, placebo-controlled study. A total of 30 participants completed the study wherein the inter vention group (n = 17) received a mixture supplement containing 600 mg/day of γ-PGA and 100 mg/day of vitamin B6, while the control group (n = 13) received a placebo. Paired datasets (baseline and endpoint data) of microbiota profiles, which were constructed via urinar y assays of microbe-derived extracellular vesicles, were analyzed and compared between the two groups. RESULTS: Only the inter vention group yielded significant results for the Bray-Curtis and Jaccard dissimilarity indices between baseline and endpoint data (P < 0.05). In the phylum- level analysis of microbial composition, the Firmicutes to Bacteroidetes ratio (FB ratio) tended to decrease from baseline in the inter vention group; however, it increased in the control group. Differences between the baseline and endpoint FB ratios were significant between the two groups (P < 0.05). CONCLUSION: This study’s findings suggest that γ-PGA-vitamin B6 supplementation potentially alters the microbial community composition of a host. Further investigation into the biological consequences of commensal microbiota alteration by γ-PGA-containing supplement consumption is warranted.
참고문헌 (32)
- Shih IL, "The production of poly-(gamma-glutamic acid)from microorganisms and its various applications", Bioresour Technol, 79, pp.207~225 (2001)
- Tanimoto H, "Acute effect of poly-gamma-glutamic acid on calcium absorption in post-menopausal women", J Am Coll Nutr, 26, pp.645~649 (2007)
- Lee SW, "Immunomodulatory effect of poly-γ-glutamic acid derived from Bacillus subtilis on natural killer dendritic cells", Biochem Biophys Res Commun, 443, pp.413~421 (2014)
- Oyunbileg E, "Effects of poly–gamma–glutamic acid on inflammatory and metabolic biomarkers in sleep–restricted rats", Sleep Biol Rhythms, 16, pp.399~404 (2018)
- Araki R, "The possibility of suppression of increased postprandial blood glucose levels by gamma-polyglutamic acid-rich natto in the early phase after eating : a randomized crossover pilot study", Nutrients, 12, pp.915~ (2020)
- García-García C, "Effects of poly-gamma-glutamic acid and vitamin B6 supplements on sleep status : a randomized intervention study", Nutr Res Pract, 15, pp.309~318 (2021)
- Jin HE, "Prebiotic effects of poly-gamma-glutamate on bacterial flora in murine gut", J Microbiol Biotechnol, 27, pp.412~415 (2017)
- Umeda K, "Combination of poly-γ-glutamic acid and galactooligosaccharide improves intestinal microbiota, defecation status, and relaxed mood in humans : a randomized, double-blind, parallel-group comparison trial", Biosci Microbiota Food Health, 42, pp.34~48 (2023)
- Liang X, "Gut bacterial extracellular vesicles : important players in regulating intestinal microenvironment", Gut Microbes, 14, pp.2134689~ (2022)
- Samra M, "Urine bacteria-derived extracellular vesicles and allergic airway diseases in children", Int Arch Allergy Immunol, 178, pp.150~158 (2019)
- Park JY, "Bacteria-derived extracellular vesicles in urine as a novel biomarker for gastric cancer: integration of liquid biopsy and metagenome analysis", Cancers(Basel), 13, pp.4687~ (2021)
- Grange C, "Exploring the role of urinary extracellular vesicles in kidney physiology, aging, and disease progression", Am J Physiol Cell Physiol, 325, pp.C1439~50 (2023)
- Kaisanlahti A, "Bacterial extracellular vesicles-brain invaders? A systematic review", Front Mol Neurosci, 16, pp.1227655~ (2023)
- 2020 Dietary Reference Intakes for Koreans, "Ministry of Health and Welfare", Ministry of Health and Welfare, ~ (2020)
- Current Status of Functional Ingredients for Health Functional Food in Korea, "Ministry of Food and Drug Safety", Ministry of Food and Drug Safety, ~ (2016)
- Shin C, "Bacterial extracellular vesicle composition in human urine and the 10-year risk of abdominal obesity", Metab Syndr Relat Disord, 21, pp.233~242 (2023)
- Sommer F, "The resilience of the intestinal microbiota influences health and disease", Nat Rev Microbiol, 15, pp.630~638 (2017)
- Manichanh C, "Reduced diversity of faecal microbiota in Crohn’s disease revealed by a metagenomic approach", Gut, 55, pp.205~211 (2006)
- Turnbaugh PJ, "A core gut microbiome in obese and lean twins", Nature, 457, pp.480~484 (2009)
- Lambeth SM, "Composition, diversity and abundance of gut microbiome in prediabetes and type 2 diabetes", J Diabetes Obes, 2, pp.1~7 (2015)
- Chen LL, "Gut microbiota in psychiatric disorders : a systematic review", Psychosom Med, 83, pp.679~692 (2021)
- Wang Z, "The microbiota-gut-brain axis in sleep disorders", Sleep Med Rev, 65, pp.101691~ (2022)
- Spychala MS, "Age-related changes in the gut microbiota influence systemic inflammation and stroke outcome", Ann Neurol, 84, pp.23~36 (2018)
- Crovesy L, "Profile of the gut microbiota of adults with obesity : a systematic review", Eur J Clin Nutr, 74, pp.1251~1262 (2020)
- Morwani-Mangnani J, "Gut microbiome changes due to sleep disruption in older and younger individuals: a case for sarcopenia?", Sleep (Basel), 45, pp.239~ (2022)
- Poroyko VA, "Chronic sleep disruption alters gut microbiota, induces systemic and adipose tissue inflammation and insulin resistance in mice", Sci Rep, 6, pp.35405~ (2016)
- Gottesmann C, "GABA mechanisms and sleep", Neuroscience, 111, pp.231~239 (2002)
- Yu X, "Wakefulness is governed by GABA and histamine cotransmission", Neuron, 87, pp.164~178 (2015)
- Sarasa SB, "A brief review on the non-protein amino acid, gamma-amino butyric acid(GABA) : its production and role in microbes", Curr Microbiol, 77, pp.534~544 (2020)
- Park KB, "Enhancement of gamma-aminobutyric acid production in Chungkukjang by applying a Bacillus subtilis strain expressing glutamate decarboxylase from Lactobacillus brevis", Biotechnol Lett, 28, pp.1459~1463 (2006)
- Yoon DW, "Oral administration of human-gut-derived Prevotella histicola improves sleep architecture in rats", Microorganisms, 11, pp.1151~ (2023)
- Yang J, "A new horizon of precision medicine : combination of the microbiome and extracellular vesicles", Exp Mol Med, 54, pp.466~482 (2022)