Effect of red chicory extract on scopolamine-induced memory impairment in mice
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We investigated the protective effects and mechanisms of red chicory extract (RCE) on scopolamine-induced memory impairment in mice. Antioxidant effects were investigated by measuring DPPH radical scavenging activity, and cell viability and intracellular ROS levels were examined by inducing cytotoxicity with L-glutamate in HT-22 cells. In addition, mice were administered orally with RCE (150 and 300 mg/kg). Memory was evaluated using Morris water maze (MWZ) and passive avoidance tests. Administration of SRCE 150 kg/mg significantly increased escape latency time (p<0.05) that was shortened by scopolamine. It further increased platform crossing numbers after 7 days of training in MWZ tests (p<0.05), which were significantly shortened by scopolamine, and prolonged latency (p<0.01) in passive avoidance tests. Biochemical findings showed significantly increased total glutathione (GSH) (p<0.05), glutathione S-transferase (GST) (p<0.01), and glutathione peroxidase (GPx) (p<0.01) in the mice administered RCE 150 mg/kg compared with the scopolamine-induced positive control. Glutathione reductase (GR) did not significantly differ between the RCE 300 mg/kg and scopolamine-induced groups (p<0.05). These results suggested that RCE has antioxidant activity and enhances scopolamine-induced cognitive impairment by attenuating oxidative stress.
Abstract
We investigated the protective effects and mechanisms of red chicory extract (RCE) on scopolamine-induced memory impairment in mice. Antioxidant effects were investigated by measuring DPPH radical scavenging activity, and cell viability and intracellular ROS levels were examined by inducing cytotoxicity with L-glutamate in HT-22 cells. In addition, mice were administered orally with RCE (150 and 300 mg/kg). Memory was evaluated using Morris water maze (MWZ) and passive avoidance tests. Administration of SRCE 150 kg/mg significantly increased escape latency time (p<0.05) that was shortened by scopolamine. It further increased platform crossing numbers after 7 days of training in MWZ tests (p<0.05), which were significantly shortened by scopolamine, and prolonged latency (p<0.01) in passive avoidance tests. Biochemical findings showed significantly increased total glutathione (GSH) (p<0.05), glutathione S-transferase (GST) (p<0.01), and glutathione peroxidase (GPx) (p<0.01) in the mice administered RCE 150 mg/kg compared with the scopolamine-induced positive control. Glutathione reductase (GR) did not significantly differ between the RCE 300 mg/kg and scopolamine-induced groups (p<0.05). These results suggested that RCE has antioxidant activity and enhances scopolamine-induced cognitive impairment by attenuating oxidative stress.
참고문헌 (23)
- Alzheimer’s Association, "2016 Alzheimer’s disease facts and figures", Alzheimers Dement, 12, pp.459~509 (2016)
- Baek SY, "Neuroprotective effect of carotenoid-rich Enteromorpha prolifera extract via TrkB", Akt pathway against oxidative stress in hippocampal neuronal cells, 2020, pp.18, 372~
- Baek SY, "Enteromorpha prolifera extract improves memory in scopolamine-treated mice via downregulating amyloid-β expression and upregulating BDNF", TrkB Pathway, 2020, pp.9, 620~
- Bais HP, "Cicorium intybus L-cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospects", J Sci Food Agric, 81, pp.467~484 (2001)
- Bartus RT, "On neurodegenerative diseases, models, and treatment strategies : Lessons learned and lessons forgotten a generation following the cholinergic hypothesis", Exp Neurol, 163, pp.495~529 (2000)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Diplock AT, "Functional food science and defense against reactive oxidative species", Br J Nutr, 80, pp.77~112 (1998)
- Ebert U, "Scopolamine model of dementia : Electroencephalogram findings and cognitive performance", Eur J Clin Invest, 28, pp.944~949 (1998)
- Elstner EF, "Oxygen radicals-Biochemical basis for their efficacy", Klin Wochenschr, 69, pp.949~956 (1991)
- Froelich L, "Free Radical mechanisms in dementia of Alzheimer type and the potential for antioxidative treatment", Arzneim-Fosch, 45, pp.443~446 (1995)
- Fukui M, "Mechanism of glutamate-induced neurotoxicity in HT-22 mouse hippocampal cells", Eur J Pharmacol, 617, pp.1~11 (2009)
- Habig WH, "Glutathione S-transferases. The first enzymatic step in mercapturic acid formation", J Biol Chem, 249, pp.7130~7139 (1974)
- Heimler D, "Polyphenol content and antioxidant activity in some species of freshly consumed salads", J Agric Food Chem, 55, pp.1724~1729 (2007)
- Lu MC, "Ameliorating effect of emodin, a constitute of Polygonatum multiflorum, on cycloheximide-induced impairment of memory consolidation in rats", J Ethnopharmacol, 112, pp.552~556 (2007)
- Nabavi SM, "Free radical scavenging and antioxidant activities of Dorema aitchisonii", J Food Drug Anal, 20, pp.34~40 (2012)
- Raza H, "Dual localization of glutathione S-transferase in the cytosol and mitochondria : Implications in oxidative stress, toxicity and disease", The FEBS J, 278, pp.4243~4251 (2011)
- Rispoli V, "Choline pivaloyl esters improve in rats cognitive and memory performances impaired by scopolamine treatment or the lesions of the nucleus basalis of Meynert", Neurosci Lett, 356, pp.199~202 (2004)
- Rossetto M, "Red chicories as potent scavengers of highly reactive radicals : A study on their phenolic composition and peroxyl radical trapping capacity and efficiency", J Agric Food Chem, 53, pp.8169~8175 (2005)
- Saharan S, "The emerging role of glutathione in Alzheimer’s disease", J Alzheimer’s Dis, 40, pp.519~529 (2014)
- Shin SK, "Mulberry fruit improves memory in scopolamine-treated mice: Role of cholinergic function, antioxidant system, and TrkB", Akt signaling, 2019, 1~11
- Tang KS, "The cellular and molecular processes associated with scopolamine-induced memory deficit : A model of Alzheimer’s biomarkers", Life Sci, 233, pp.116695~ (2019)
- Valis M, "The concentration of memantine in the cerebrospinal fluid of Alzheimer’s disease patients and its consequence to oxidative stress biomarkers", Front Pharmacol, 10, pp.943~ (2019)
- Zanoni F, "Microencapsulation by spray-drying of polyphenols extracted from red chicory and red cabbage : Effects on stability and color properties", Food Chem, 307, pp.125535~ (2020)