차나무 잎의 캘러스 배양을 통한 카테킨류의 생산성 개선

Abstract

The effects of thiamine-HCl or polyvinylpyrrolidone (PVP) on growth and catechincontents of calli from Camellia sinensisleaves were investigated to improve catechinproductivity in callus cultures on the selective medium. The growth of calli was great on theproliferation medium (the MS medium with 0.2 ㎎/L 2,4-D and 1.0 ㎎/L TDZ)supplemented with 20∼30 ㎎/L thiamine-HCl. Although the caffeine content was low incalli compared to young leaves, the total content of catechins was high in calli. Particularly,(-)-epicatechin (EC) which not detected in young leaves was also detected in calli on thepropagation medium containing thiamine-HCl or PVP. In addition, the (-)-epicatechingallate (ECG) content was significantly higher in calli than in young leaves. Inconclusion, the proliferation medium supplemented with 30 ㎎/L thiamine-HCl as a vitaminsource seemed to be optimal condition for the growth and catechin production in callusculture.