자색고구마 습관성캘러스로부터 안토시아닌 고생합성 세포주 선발

Abstract

Callus induction using leaf of purple sweetpotato (PSP) was decreased when subcultured. So we selected habituated callus in MS medium supplemented with 1 µM 2,4-D (2,4-dichlorophenoxyacetic acid) after 6 months of cultures (without subculture). It grew faster and easier than any other callus. It was able to proliferate in MS hormone free solid and liquid medium without any growth regulators and subculture limits. During subculture in liquid medium, a purple mottled spot formed in one of habituated cell aggregates without any treatment. This purple cell aggregates were carefully separated from habituated cell aggregates, and then subcultured by selecting purple cell aggregates for more than 2 years to be isolated. The color value of the pigment extracted of culture was 1.0 mg/mL, which was close to that of a pigment extracted from storage root, which was 1.5 mg/mL. This purple cell aggregates could therefore be used for the industrial mass production of anthocyanin.