본문 바로가기
KERD
논문KCI 등재

헐떡이풀의 핵형분석과 Bicolor FISH를 이용한 물리적 지도 작성

Karyotype Analysis and Physical Mapping of rDNAs UsingBicolor-FISH in Tiarella polyphylla D. Don

김수영|이중구
한국자원식물학회 (The Plant Resources Society Of Korea)|2007.10|한국자원식물학회지 (Korean Journal of Plant Reources.)|제20권 제5호|pp. 446-450

0

조회

0

다운로드

1

인용

0

참고문헌

초록

Abstract

- Tiarella polyphyllaD. Don (Saxifragaceae) is a perennial herb and distributed in China, Japan, Taiwan andKorea. Especially, it only grows in Ulleung island of Korea. It has been using for asthma, bruise and audition troubleswith main components of some Triterpenoids and seven oleanolic Saponins. There is only known its chromosomalnumber rarely and cytogenetic study was not done. From this study, karyotype analysis and chromosomal localization of5S and 45S rDNAs using bicolor-FISH (fluorescence in situhybridization) were carried out. The somatic metaphasechromosome number was 2n=2x=14 and the size of chromosomes ranged 1.66∼3.50㎛. The chromosome complementconsisted of four pairs of submetacentrics (chromosomes 1, 2, 3 and 6), two pairs of subtelocentrics (chromosomes 5 and7) and one pair of telocentrics (chromosome 4). We also observed NOR (nucleolus organizer region) on the chromosome4. In bicolor-FISH, one pair of 55 and 45S rDNA sites was detected on the centromeric region of chromosome 3 andshort arm of chromosome 4, respectively. Bicolor FISH was very useful tool for the localization and identification ofrDNAs on the chromosomes in Tiarella polyphylla.Key words - Tiarella polyphylla, Karyotype analysis, Bicolor-FISH, 5S rDNA, 45S rDNA

참고문헌 (21)

  1. Benabdelmouna, A, "Distribution and chromosomal organization of 18S-5.8S-25S and 5S rDNA in Petuniaspecies", 19, pp.349~360 (1997)
  2. Castilho, A, "Physical mapping of 5S and 18S-25S rDNA and repetitive DNA sequences in Aegilops umbellulata", 38, pp.91~96 (1995)
  3. D’Hont, "Determination of basic chromosome numbers in the genus Saccharumby physical mapping of ribosomal RNA genes", 41, pp.221~225 (1998)
  4. Fukui, "N. Ohmido and G.S. Khush. 1994. Variability in rDNA lociin the genus Oryzadetected through fluorescence in situhybridization. Theor. Appl. Genet. 87", pp.893~899
  5. . Chromosome Information Service 51, "T. and T. Nakamura. 1991. Karyomorphological study on Japanese Tiarella polyphylla", pp.14~15
  6. Hasterok, R, "Ribosomal DNA is an effective marker of Brassica chromosomes", 103, pp.486~490 (2001)
  7. Han, Y. H, "Physical mapping of the 5S and 45S rDNA in teosintes", 137, pp.16~19 (2002)
  8. Kim, S.Y, "Physical mapping of rDNAs using McFISH in Anemarrhena asphodeloides Bunge", 12, pp.515~518 (2004)
  9. Kim, S.Y, "Karyotype analysis and physical mapping of rDNAs using McFISH in Jeffersonia dubiaBenth", 13, pp.48~51 (2005)
  10. Kim, S.Y, "Cytogenetic analysis of Astragalusspecies", 14, pp.250~254 (2006)
  11. Kim, S.Y, "Cytogenetic analysis using mitosis, meiosis chromosomes and bicolor fluorescence in situ hybridization of Bupleurum latissimumNakai", 14, pp.354~359 (2006)
  12. Kim, S.Y, "Characterization of eight Rumexspecies by FISH (fluorescence in situhybridization) and 5S rDNA spacer sequences", 28, pp.528 243~251 (2006)
  13. Koo, D.H, "Karyotype analysis and physical mapping of rDNAs in Bupleurum longeradiatum", 11, pp.402~407 (2003)
  14. "S. 1966. Cytological studies on some Eastern Himalayanplants. In The flora of Eastern Himalaya Complied by Hitoshi Hava Univ. of Tokyo Press. Japan", pp.658~670
  15. Lee, W.K, "Molecular cytogenetics of five Pulsatillaspecies to the 5S, 45S rDNA genes by fluorescence in situhybridization", 27, pp.179~185 (2005)
  16. Lee, "T.B. 1980. Illustrated Flora of Korea. Hyangmoonsa Co.", Ltd
  17. Leitch, "I.J. and J.S. Heslop-Harrison. 1992. Physical mapping of the18S-5.8S-26S rDNA genes in barley by in situhybridization.Genome 53", pp.1013~1018
  18. Levan, "K. Frekga and A. Sandberg. 1964. Nomenclature forcentromeric position in chromosomes. Hereditas 52", pp.201~220
  19. "J. and J.S. Heslop-Harrison. 1991. Localization oftandemly repeated DNA sequences in Arabidopsis thaliana. Plant J.1", pp.159~166
  20. Matsuura, "H. and T. Suto. 1935. Contributions to the ideogram study inPhanerogamous plants I. Jour. Fac. Sci. Hokkaido Imp. Univ. Ser. 5", pp.33~5 75
  21. 박시형, "Structure Determination of a New Lupane-type Triterpene, Tiarellic Acid, Isolated from Tiarella polyphylla", Archives of Pharmacal Research, 25(1), pp.57~60 (2002), 대한약학회

관련 논문AI추천

깽깽이풀의 핵형분석과 McFISH를 이용한 rDNA의 물리지도 작성구달회, 김수영, 김찬수, 방재욱, 최혜운한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제1호개시호(Bupleurum longeradiatum)의 핵형분석과 rDNAs의 Physical Mapping구달회, 방경환, 방재욱, 성낙술, 성정숙한국약용작물학회 · 한국약용작물학회지2003 · 제11권 제5호2배체 담배 Nicotiana plumbaginifolia의 핵형 분석과 rDNAs의 Physical Mapping구달회, 김수영, 방재욱, 조혜경한국식물생명공학회 · Journal of Plant Biotechnology2003 · 제30권 제1호할미꽃속 식물 5종의 핵형 분석이우규한국약용작물학회 · 한국약용작물학회지2004 · 제12권 제6호Multicolor FISH와 Feulgen 염색법을 이용한 Angelica속 식물의 세포유전학적 분석구달회, 김수영, 방경환, 방재욱, 성낙술한국식물생명공학회 · Journal of Plant Biotechnology2003 · 제30권 제2호쪽의 핵형분석과 rRNA 유전자의 염색체상 위치김수영, 방재욱, 이상훈, 최혜운한국약용작물학회 · 한국약용작물학회지2008 · 제16권 제3호

이 간행물의 다른 논문 (15건)

석불산 일대의 관속식물 분포와 군락 분류오현경, 변무섭pp. 375-382셀레늄 시용에 따른 녹두 생육 및 Se 함량 변화정병준, 이경동, 임요섭, 손동모, 김동관, 김관수, 천상욱pp. 383-388벼 조직배양 기간에 따른 retrotransposon(Tos17)의 활성에 관한 연구홍순관, 양희은, 방일란, 이병진, 신영범pp. 389-397침지 온도와 시간이 삼나무 종자의 수분흡수 및 발아에 미치는 영향탁우식, 조경진, 최충호pp. 398-403참깨의 연작장해 경감 녹비작물 선발 및 윤작효과노창우, 남상영, 강효중, 민경범, 김인재, 이철희, 윤태, 김민자pp. 404-408
원문 보기